Integral evaluation for intermittent cold storage of apples by using mathematical modeling

2021 ◽  
Author(s):  
Jia‐Wei Han ◽  
Zeng‐Tao Ji ◽  
Min Zuo ◽  
Xin‐Ting Yang
Keyword(s):  
Mathematical Modeling ◽  
Cold Storage ◽  
Integral Evaluation

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

Survival of partial liver grafts is increased after cold storage in Custodiol-N (HTK-N)

2010 ◽  
Vol 48 (01) ◽  
Author(s):  
Q Liu ◽  
DP Schultze ◽  
H Bruns ◽  
M Zorn ◽  
M Büchler ◽  
...  
Keyword(s):  
Cold Storage

Cerebral Blood Circulation in Premature Infants by Means of Mathematical Modeling

2015 ◽  
Vol 46 (S 01) ◽  
Author(s):  
R. Lampe ◽  
N. Botkin ◽  
V. Turova ◽  
T. Blumenstein ◽  
A. Alves-Pinto
Keyword(s):  
Mathematical Modeling ◽  
Premature Infants ◽  
Blood Circulation ◽  
Cerebral Blood Circulation
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