Characterization of Edwardsiella piscicida CK108 flagellin genes and evaluation of their potential as vaccine targets in the zebrafish model

2021 ◽  
Author(s):  
Yunjeong Choe ◽  
Donghee Lee ◽  
Minji Seong ◽  
Ju Bin Yoon ◽  
Jun Hyeok Yang ◽  
...  
Keyword(s):  
Zebrafish Model ◽  
Edwardsiella Piscicida

scholarly journals Further characterization of the zebrafish model of acrylamide acute neurotoxicity: gait abnormalities and oxidative stress

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Melissa Faria ◽  
Arnau Valls ◽  
Eva Prats ◽  
Juliette Bedrossiantz ◽  
Manuel Orozco ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Zebrafish Model ◽  
Acute Neurotoxicity ◽  
Gait Abnormalities ◽  
And Oxidative Stress

Characterization of the Japanese flounder NLRP3 inflammasome in restricting Edwardsiella piscicida colonization in vivo

2020 ◽  
Vol 103 ◽  
pp. 169-180
Author(s):  
Hao Chen ◽  
Shuangfei Ding ◽  
Jinchao Tan ◽  
Dahai Yang ◽  
Yuanxing Zhang ◽  
...  
Keyword(s):  
Nlrp3 Inflammasome ◽  
Japanese Flounder ◽  
Edwardsiella Piscicida

scholarly journals Zebrafish larvae as a model system for systematic characterization of drugs and genes in dyslipidemia and atherosclerosis

2018 ◽  
Author(s):  
Manoj K Bandaru ◽  
Anastasia Emmanouilidou ◽  
Petter Ranefall ◽  
Benedikt von der Heyde ◽  
Eugenia Mazzaferro ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Large Scale ◽  
Early Stage ◽  
Model System ◽  
Concomitant Treatment ◽  
Induced Mutations ◽  
Zebrafish Model ◽  
Disease Etiology ◽  
Causal Genes

AbstractBackgroundHundreds of loci have been robustly associated with circulating lipids, atherosclerosis and coronary artery disease; but for most loci the causal genes and mechanisms remain uncharacterized.MethodsWe developed a semi-automated experimental pipeline for systematic, quantitative, large-scale characterization of mechanisms, drugs and genes associated with dyslipidemia and atherosclerosis in a zebrafish model system. We validated our pipeline using a dietary (n>2000), drug treatment (n>1000), and genetic intervention (n=384), and used it to characterize three candidate genes in a GWAS-identified pleiotropic locus on chr 19p13.11 (n>500).ResultsOur results show that five days of overfeeding and cholesterol supplementation had independent pro-atherogenic effects, which could be diminished by concomitant treatment with atorvastatin and ezetimibe. CRISPR-Cas9-induced mutations in orthologues of proof-of-concept genes resulted in higher LDL cholesterol levels (apoea), and more early stage atherosclerosis (apobb.1). Finally, our pipeline helped identify putative causal genes for circulating lipids and early-stage atherosclerosis (LPAR2andGATAD2A).ConclusionsIn summary, our pipeline facilitates systematic,in vivocharacterization of drugs and candidate genes to increase our understanding of disease etiology, and can likely help identify novel targets for therapeutic intervention.

scholarly journals Loss-of-function of the NOMO1 Gene Causes Neuropsychiatric Disorder-related Phenotypes

2020 ◽  
Author(s):  
Fei Li ◽  
Tingting Li ◽  
Jia Lin ◽  
Jing Jian ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Neuropsychiatric Disorders ◽  
Repetitive Behaviors ◽  
Loss Of Function ◽  
Spontaneous Movement ◽  
Zebrafish Model ◽  
Behavioral Phenotypes ◽  
Functional Mechanism ◽  
Genome Wide ◽  
Vertebrate Model

Abstract Background: Clinical genome-wide analysis identified NOMO1 in human chromosome 16p13.11 as a candidate gene associated with neuropsychiatric disorders such as autism, schizophrenia and epilepsy. However, the important contributions underlying NOMO1 deficiency resulting in neuropsychiatric disorders is not understand, and the molecular and pathogenesis mechanisms of nomo1 gene are unclear. Therefore, it is necessary to construct animal models to systematically study the effects of nomo1 deficiency on neuropsychiatric system and explore pathogenic molecular mechanism of diseases.Methods: We developed a viable vertebrate model of loss-of-function of nomo1 using CRISPR/Cas9 and studied the characterization of nomo1 mutant zebrafish. Phenotypic research was performed in developing nomo1 mutant zebrafish, including morphological measurements, behavioral tests, and functional mechanism analyses.Results: The nomo1 loss-of-function zebrafish model accurately recapitulated key neuropsychiatric disorders traits. The mutant zebrafish showed decreased locomotion in the larval stage (7 dpf), increased spontaneous movement in infancy (15 dpf and 30 dpf), and social defects and repetitive behaviors in adolescence (2 mpf). More importantly, we demonstrated that these behavioral phenotypes stem from abnormal brain structure and neurotransmitter metabolism. Transcriptome analysis provided insights for studying the functional mechanism of nomo1 pathogenesis. Further results revealed that the neuroactive drug PTZ recovered the decreased locomotion phenotype in larval zebrafish, which provides functional basis for the exploration of drug sensitivity and intervention in behavioral phenotypes of nomo1 mutant zebrafish.Conclusion: This study firstly reveal the functional evidence that loss-of-function of nomo1 elicits neuropsychiatric disorders, and emphasize the relationship between nomo1 deficiency and neuropsychiatric disorders from the perspectives of behavioral phenotypes, brain development, and neurotransmitter metabolism.Limitation: The behavioral phenotype intervention of neuroactive drug in nomo1-/-zebrafish can be directly translated to the behavior of human associated diseases need further study.

Deep characterization of a zebrafish model for dominant osteogenesis imperfecta

2016 ◽  
Author(s):  
Francesca Tonelli ◽  
Roberta Gioia ◽  
Marco Biggiogera ◽  
Shannon Fisher ◽  
Sergey Leikin ◽  
...  
Keyword(s):  
Osteogenesis Imperfecta ◽  
Zebrafish Model

scholarly journals Zebrafish xenografts to isolate unique human breast cancer metastatic cell populations

2021 ◽  
Author(s):  
Jerry Xiao ◽  
Joseph R. McGill ◽  
Apsra Nasir ◽  
Alexander Lekan ◽  
Bailey Johnson ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Cancer Metastasis ◽  
Circulatory System ◽  
Cell Populations ◽  
Protein Protein Interactions ◽  
Zebrafish Model ◽  
Metastatic Cell ◽  
Metastatic Cells

Cancer metastasis is a critical culprit frequently blamed for treatment failure, drug resistance, poor prognosis, and high mortality rate among all human cancers. Laboratory efforts to isolate metastatic cell populations have typically been confined to mouse models, which are time-consuming and expensive. Here, we present a model system based on xenografting zebrafish embryos to select for cells that are predisposed to progress through the early stages of metastasis. This model requires only 3-5 days to achieve distinct intravasation to the zebrafish circulatory system. The metastatic cells are easily tracked in real-time as they migrate, as well as isolated and propagated in vitro. Once expanded, molecular characterization of the serially derived invasive cell populations from the tails of the zebrafish accurately predicts genes, signaling pathways, protein-protein interactions, and differential splicing products that are important for an invasive phenotype. This zebrafish model therefore offers a high-throughput and robust method for identifying gene targets critical for cancer metastasis.

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