Establishment and characterization of a liver cell line, ALL, derived from yellowfin sea bream, Acanthopagrus latus , and its application to fish virology

2021 ◽  
Author(s):  
Yong Li ◽  
Peng jia ◽  
Fangzhao Yu ◽  
Wangdong Li ◽  
Can Mao ◽  
...  
Keyword(s):  
Cell Line ◽  
Liver Cell ◽  
Sea Bream ◽  
Liver Cell Line ◽  
Acanthopagrus Latus

Establishment and characterization of a fin cell line from yellowfin sea bream (Acanthopagrus latus) and its application to fish virology and toxicology

Aquaculture ◽  
2022 ◽  
Vol 549 ◽  
pp. 737801
Author(s):  
Peng Jia ◽  
Fanming Jin ◽  
Yangxi Xiang ◽  
Jianhuan Li ◽  
Hongbo Pan ◽  
...  
Keyword(s):  
Cell Line ◽  
Sea Bream ◽  
Acanthopagrus Latus

Derivation and characterization of a zebrafish liver cell line

1994 ◽  
Vol 10 (3) ◽  
pp. 167-176 ◽  
Author(s):  
C. Ghosh ◽  
Y. L. Zhou ◽  
P. Collodi
Keyword(s):  
Cell Line ◽  
Liver Cell ◽  
Liver Cell Line ◽  
Zebrafish Liver

Development and characterization of a rainbow trout liver cell line expressing cytochrome P450-dependent monooxygenase activity

1993 ◽  
Vol 9 (3) ◽  
pp. 279-294 ◽  
Author(s):  
Lucila E. J. Lee ◽  
Janine H. Clemons ◽  
Daniel G. Bechtel ◽  
Sarah J. Caldwell ◽  
Kyu-Bo Han ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rainbow Trout ◽  
Cell Line ◽  
Liver Cell ◽  
Monooxygenase Activity ◽  
Liver Cell Line ◽  
Rainbow Trout Liver

Functional characterization of copper transporters zCtr1, zAtox1, zAtp7a and zAtp7b in zebrafish liver cell line ZFL

Metallomics ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1532-1546 ◽  
Author(s):  
Man Long Kwok ◽  
King Ming Chan
Keyword(s):  
Cell Line ◽  
Liver Cell ◽  
Functional Characterization ◽  
Essential Element ◽  
Cellular Activity ◽  
Liver Cell Line ◽  
Copper Transporters ◽  
Zebrafish Liver

Copper (Cu) is an essential element for all organisms, serving as an enzyme cofactor to maintain cellular activity and vitality.

Cytological, immunocytochemical, ultrastructural and growth characterization of the rainbow trout liver cell line RTL-W1

2013 ◽  
Vol 45 (3) ◽  
pp. 159-174 ◽  
Author(s):  
F. Malhão ◽  
R. Urbatzka ◽  
J.M. Navas ◽  
C. Cruzeiro ◽  
R.A.F. Monteiro ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Cell Line ◽  
Liver Cell ◽  
Liver Cell Line ◽  
Growth Characterization ◽  
Rainbow Trout Liver

scholarly journals Glycogen phosphorylase isoenzymes from hepatoma 3924A and from a non-tumorigenic liver cell line. Comparison with the liver and brain enzymes

1992 ◽  
Vol 282 (3) ◽  
pp. 665-673 ◽  
Author(s):  
D Mayer ◽  
G Seelmann-Eggebert ◽  
I Letsch
Keyword(s):  
Cell Line ◽  
Liver Cell ◽  
Metabolic Regulation ◽  
Glycogen Phosphorylase ◽  
Main Band ◽  
Liver Cell Line ◽  
Normal Rat ◽  
Morris Hepatoma ◽  
The Brain

Glycogen phosphorylase isoenzymes were isolated from normal rat liver, rat brain, the glycogen-poor Morris hepatoma (MH) 3924A, and the glycogen-rich non-tumorigenic liver cell line C1I. Electrophoretic and immunological characterization of the enzymes showed that tumour and C1I cells expressed a phosphorylase isoform similar to the brain type; the liver type was not detectable. All enzymes were obtained as dimers; the Mr of the subunits was 96,000 (liver), 93,000 (brain and MH 3924A) and 92,000 (C1I). Isoelectric focusing revealed a main band of pI 6.34 for liver phosphorylase a, pI 5.67 for the enzymes from MH 3924A and brain, and pI 5.68 for C1I phosphorylase. Partial kinetic characterization of the AMP-independent forms of the isoenzymes yielded Km values for glucose 1-phosphate of 3.5 +/- 0.5 mM (liver), 3.9 mM (brain), 1.9 +/- 0.3 mM (MH 3924A) and 2.5 +/- 0.5 mM (C1I); Km values for glycogen were 0.4 mM (liver) and 0.3 mM (MH 3924A and C1I), calculated as glucose equivalents. The AMP-independent phosphorylase was inhibited by glucose 6-phosphate (Glc6P) with Ki values of 0.32 +/- 0.03 mM (C1I), 0.50 +/- 0.04 mM (MH 3924A) and approximately 5 mM (brain). The inhibition could be abolished by 1 mM-AMP, indicating that AMP and Glc6P may partially compete for the same site on the protein. Liver phosphorylase a was not inhibited by up to 25 mM-Glc6P. In contrast with liver and brain isoenzymes, phosphorylase from the cell lines was not affected by NaF and Na2SO4. The data show that both the hepatocellular carcinoma and the non-malignant immortalized liver cells express a phosphorylase isoform different from the liver type. Furthermore, there is some evidence that the enzyme from MH 3924A and C1I cells is distinct from brain phosphorylase a, in spite of electrophoretic and immunological resemblance, and that this isoenzyme is subject to altered metabolic regulation.

Characterization of the mouse liver cell line FL83B

1973 ◽  
Vol 78 (2) ◽  
pp. 441-453 ◽  
Author(s):  
J.L. Breslow ◽  
H.R. Sloan ◽  
V.J. Ferrans ◽  
J.L. Anderson ◽  
R.I. Levy
Keyword(s):  
Cell Line ◽  
Liver Cell ◽  
Mouse Liver ◽  
Liver Cell Line ◽  
Mouse Liver Cell

Characterization of Ah receptor promoter in human liver cell line, HepG2

1994 ◽  
Vol 4 (4) ◽  
pp. 219-222 ◽  
Author(s):  
Yoshiki Takahashi ◽  
Susumu Itoh ◽  
Tsukasa Shimojima ◽  
Tetsuya Kamataki
Keyword(s):  
Cell Line ◽  
Liver Cell ◽  
Human Liver ◽  
Ah Receptor ◽  
Liver Cell Line ◽  
Human Liver Cell
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