Antiviral activity of esculin against white spot syndrome virus: A new starting point for prevention and control of white spot disease outbreaks in shrimp seedling culture

2021 ◽  
Author(s):  
Li‐Peng Shan ◽  
Xu Zhang ◽  
Yang Hu ◽  
Lei Liu ◽  
Jiong Chen
Keyword(s):  
Antiviral Activity ◽  
Prevention And Control ◽  
White Spot Syndrome Virus ◽  
Disease Outbreaks ◽  
White Spot ◽  
White Spot Disease ◽  
Spot Disease ◽  
Starting Point ◽  
White Spot Syndrome ◽  
And Control

scholarly journals Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection

2020 ◽  
Vol 19 (2) ◽  
pp. 118-130
Author(s):  
Widanarni Widanarni ◽  
Dewi Rahmi ◽  
Muhamad Gustilatov ◽  
Sukenda Sukenda ◽  
Diah Ayu Satyari Utami
Keyword(s):  
Immune Response ◽  
White Spot Syndrome Virus ◽  
Lethal Dose ◽  
White Spot ◽  
White Shrimp ◽  
Average Weight ◽  
Bacillus Sp ◽  
White Spot Disease ◽  
Spot Disease ◽  
White Spot Syndrome

ABSTRACT   White spot disease caused by White Spot Syndrome Virus (WSSV) is the most serious viral disease and has a major impact on the decline in production of white shrimp farm. Improving the immune response through the application of prebiotic, probiotic and synbiotic is expected to be one of the environmentally friendly alternatives to prevent the disease. This study aimed to evaluate the effect of administrating Baccillus sp. NP5 probiotic, honey prebiotic and a combination both (synbiotic) in enhancing immune response and resistence of white shrimp to WSSV infection. This study consisted of five treatments and three replications, namely positive control (feeding without probiotics, prebiotics, and synbiotics then challenged with WSSV), negative control (feeding without probiotics, prebiotics, synbiotics, then injected with PBS), pro (feeding with the addition of Bacillus sp. NP5 probiotics then challenged against WSSV), pre (feeding with the addition of honey prebiotics then challenged against WSSV), and sin (feeding with the addition of synbiotics then challenged against WSSV). White shrimp with an average weight of 1.8±0.06 gram/shrimp were reared at a density of 15 shrimps per aquarium (60 cm x 30 cm x 30 cm and water height of 20 cm) for eight weeks, then challenge against WSSV at lethal dose (LD50) dose as much as 0.1 ml per shrimp. The results showed that the immune response and resistence of white shrimp after treatments of probiotics, prebiotics, and synbiotics were better than those in controls with the optimal found in prebiotics treatment.   Keywords: Bacillus sp. NP5, honey, immune response, white shrimp, WSSV     ABSTRAK   White spot disease yang disebabkan oleh infeksi white spot syndrome virus (WSSV) merupakan penyakit viral paling serius dan berdampak besar terhadap penurunan produksi budidaya udang vaname. Perbaikan respons imun melalui aplikasi probiotik, prebiotik dan sinbiotik dapat menjadi salah satu alternatif ramah lingkungan untuk pencegahan serangan penyakit tersebut. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian probiotik Bacillus sp. NP5, prebiotik madu dan gabungan keduanya (sinbiotik) dalam meningkatkan respons imun dan resistansi udang vaname terhadap infeksi WSSV. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan yaitu kontrol positif (pemberian pakan tanpa probiotik, prebiotik, dan sinbiotik kemudian diuji tantang dengan WSSV), kontrol negatif (pemberian pakan tanpa probiotik, prebiotik, dan sinbiotik kemudian diinjeksi PBS), pro (pemberian pakan dengan penambahan probiotik Bacillus sp. NP5 kemudian diuji tantang dengan WSSV), pre (pemberian pakan dengan penambahan prebiotik madu kemudian diuji tantang WSSV), dan sin (pemberian pakan dengan penambahan sinbiotik kemudian diuji tantang dengan WSSV). Udang vaname dengan bobot rata-rata 1.8±0.06 gram/ekor dipelihara dengan kepadatan 15 ekor per akuarium (60 cm x 30 cm x 30 cm) selama delapan minggu, kemudian diuji tantang dengan WSSV sebanyak 0.1 ml per ekor pada dosis LD50. Hasil penelitian menunjukkan bahwa respons imun dan resistansi udang vaname setelah pemberian probiotik, prebiotik dan sinbiotik lebih baik (P<0.05) dibandingkan kontrol dengan hasil optimal pada perlakuan prebiotik.   Kata kunci: Bacillus sp. NP5, madu, respons imun, udang vaname, WSSV.    

Anti-viral RNAi nanoparticles protect shrimp against white spot disease

2018 ◽  
Vol 3 (1) ◽  
pp. 38-48 ◽  
Author(s):  
Shai Ufaz ◽  
Adi Balter ◽  
Chen Tzror ◽  
Shai Einbender ◽  
Ori Koshet ◽  
...  
Keyword(s):  
Rna Interference ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Viral Diseases ◽  
Penaeus Vannamei ◽  
White Spot Disease ◽  
Spot Disease ◽  
White Spot Syndrome

Nearly 20% of cultured shrimp die every year due to viral diseases. In this study, we evaluated the capacity of nanoparticulate RNA interference (RNAi) to down-regulate genes in Penaeus vannamei shrimp and protect shrimp against white spot syndrome virus (WSSV, i.e. white spot disease).

Evaluation on the antiviral activity of genipin against white spot syndrome virus in crayfish

2019 ◽  
Vol 93 ◽  
pp. 380-386 ◽  
Author(s):  
Ai-Guo Huang ◽  
Xiao-Ping Tan ◽  
Shen-Ye Qu ◽  
Gao-Xue Wang ◽  
Bin Zhu
Keyword(s):  
Antiviral Activity ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
White Spot Syndrome

Antiviral activity of Cynodon dactylon on white spot syndrome virus (WSSV)-infected shrimp: an attempt to mitigate risk in shrimp farming

2020 ◽  
Vol 28 (4) ◽  
pp. 1725-1738 ◽  
Author(s):  
Pallabi Howlader ◽  
Alokesh Kumar Ghosh ◽  
Shikder Saiful Islam ◽  
Joyanta Bir ◽  
Ghausiatur Reza Banu
Keyword(s):  
Antiviral Activity ◽  
White Spot Syndrome Virus ◽  
Cynodon Dactylon ◽  
Shrimp Farming ◽  
White Spot ◽  
White Spot Syndrome

scholarly journals Mixed-genotype white spot syndrome virus infections of shrimp are inversely correlated with disease outbreaks in ponds

2010 ◽  
Vol 92 (3) ◽  
pp. 675-680 ◽  
Author(s):  
T. T. T. Hoa ◽  
M. P. Zwart ◽  
N. T. Phuong ◽  
D. T. H. Oanh ◽  
M. C. M. de Jong ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Disease Outbreaks ◽  
White Spot ◽  
Virus Infections ◽  
Mixed Genotype ◽  
White Spot Syndrome

scholarly journals Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)

Viruses ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1713
Author(s):  
Alain Moïse Onihary ◽  
Iony Manitra Razanajatovo ◽  
Lydia Rabetafika ◽  
Alexandra Bastaraud ◽  
Jean-Michel Heraud ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Macrobrachium Rosenbergii ◽  
Culture Industry ◽  
White Spot ◽  
Type I ◽  
Shrimp Culture ◽  
White Spot Disease ◽  
North West ◽  
White Spot Syndrome

White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known about the circulation of the virus and its genetic diversity. Our study aimed at describing the molecular diversity and the spread of WSSV in the populations of Madagascan crustaceans. Farmed and wild shrimps were collected from various locations in Madagascar from 2012 to 2016 and were tested for WSSV. Amplicons from positive specimens targeting five molecular markers (ORF75, ORF94, ORF125, VR14/15 and VR23/24) were sequenced for genotyping characterizations. Four genotypes were found in Madagascar. The type-I genotype was observed in the south-west of Madagascar in April 2012, causing a disastrous epidemic, then spread to the North-West coast. Type-II strains were detected in October 2012 causing an outbreak in another Penaeus monodon farm. In 2014 and 2015, types II and III were observed in shrimp farms. Finally, in 2016, types II and IV were found in wild species including Fenneropenaeus indicus, Metapenaeus monoceros, Marsupenaeus japonicus and Macrobrachium rosenbergii. Considering the economic importance of the shrimp industry for Madagascar, our study highlights the need to maintain WSSV surveillance to quickly take appropriate countermeasures in case of outbreak and to sustain this industry.

scholarly journals In Silico Prediction of Molecular Interaction Within PmCBP-VP24 Complex to Understand Initial Instigation of WSSV into Shrimps

2021 ◽  
Vol 5 (2) ◽  
pp. 87-93
Author(s):  
Kanika Yadav ◽  
Arunima Kumar Verma ◽  
Ajey Kumar Pathak ◽  
Abhishek Awasthi
Keyword(s):  
Digestive Tract ◽  
Molecular Interaction ◽  
In Silico ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Receptor Protein ◽  
In Silico Prediction ◽  
White Spot Disease ◽  
Spot Disease ◽  
Chitin Binding Protein

White Spot Disease is one of the most devastating diseases of shrimps. Molecular interaction between shrimp receptor protein PmCBP (Chitin binding protein of Peneaus monodon) and viral envelop protein VP24 is obligatory for binding of the White Spot Syndrome Virus to the shrimp digestive tract, and failure of this anchoring leads to an ineffectual infection. This is a first study that throws light on the molecular interaction of PmCBP-VP24 complex and provides important clues for initial steps of ingression of the virus into shrimps.

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