scholarly journals Stability of viral haemorrhagic septicaemia virus, infectious hematopoietic necrosis virus and cyprinid herpesvirus 3 in various water samples

2020 ◽  
Author(s):  
Johanna Ullrich ◽  
Jürgen Christian ◽  
Sven Michael Bergmann ◽  
Martin Oberle ◽  
Anna Maria Becker
Keyword(s):  
Water Samples ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Necrosis Virus ◽  
Haemorrhagic Septicaemia ◽  
Cyprinid Herpesvirus 3 ◽  
Infectious Hematopoietic Necrosis ◽  
Viral Haemorrhagic Septicaemia Virus

Concentration of Infectious Hematopoietic Necrosis Virus from Water Samples by Tangential Flow Filtration and Polyethylene Glycol Precipitation

1989 ◽  
Vol 46 (6) ◽  
pp. 964-968 ◽  
Author(s):  
W. N. Batts ◽  
J. R. Winton
Keyword(s):  
Polyethylene Glycol ◽  
Water Samples ◽  
Sockeye Salmon ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Necrosis Virus ◽  
Total Recovery ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Infectious Hematopoietic Necrosis ◽  
Flow Filtration

Infectious hematopoietic necrosis virus (IHNV) was concentrated from water samples by polyethylene glycol (PEG) precipitation, tangential flow filtration (TFF), and by a combination of TFF followed by PEG precipitation of the retentate. Used alone, PEG increased virus titers more than 200-fold, and the efficiency of recovery was as great as 100%. Used alone, TFF concentrated IHNV more than 20-fold, and average recovery was 70%. When the two techniques were combined, 10-L water samples were reduced to about 300 mL by TFF and the virus was precipitated with PEG into a 1 to 2 g pellet; total recovery was as great as 100%. The combined techniques were used to isolate IHNV from water samples taken from a river containing adult sockeye salmon (Oncorhynchus nerka) and from a hatchery pond containing adult spring chinook salmon (O. tshawytscha). The combination of these methods was effective in concentrating and detecting IHNV from water containing only three infectious particles per 10-L sample.

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