Experimental tenacibaculosis infection in adult conger eel ( Genypterus chilensis , Guichenot 1948) by immersion challenge with Tenacibaculum dicentrarchi

2020 ◽  
Author(s):  
Rute Irgang ◽  
Ruben Avendaño‐Herrera
Keyword(s):  
Conger Eel ◽  
Genypterus Chilensis

scholarly journals Microbial Communities Associated with Farmed Genypterus chilensis: Detection in Water Prior to Bacterial Outbreaks Using Culturing and High-Throughput Sequencing

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1055 ◽  
Author(s):  
Arturo Levican ◽  
Jenny C. Fisher ◽  
Sandra L. McLellan ◽  
Ruben Avendaño-Herrera
Keyword(s):  
Bacterial Community ◽  
High Throughput ◽  
Native Species ◽  
High Throughput Sequencing ◽  
Disease Outbreaks ◽  
Emerging Pathogens ◽  
Prophylactic Measures ◽  
Almost All ◽  
Conger Eel ◽  
Genypterus Chilensis

The red conger eel (Genypterus chilensis, Guichenot) is a native species included in the Chilean Aquaculture Diversification Program due to high commercial demand. In the context of intensified farming, prior reports link two disease outbreaks with emerging pathogens in the Vibrio and Tenacibaculum genera. However, the roles remain unclear for the bacterial community and each specific bacterium is associated with the rearing environment for healthy specimens. The success of red conger eel farming therefore warrants research into the bacterial composition of aquaculture conditions and the antimicrobial susceptibilities thereof. This study used culturing methods and high-throughput sequencing to describe the bacterial community associated with water in which G. chilensis was farmed. With culturing methods, the predominant genera were Vibrio (21.6%), Pseudolteromonas (15.7%), Aliivibrio (13.7%), and Shewanella (7.8%). Only a few bacterial isolates showed amylase, gelatinase, or lipase activity, and almost all showed inhibition zones to commonly-used antibiotics in aquaculture. By contrast, high-throughput sequencing established Paraperlucidibaca, Colwellia, Polaribacter, Saprospiraceae, and Tenacibaculum as the predominant genera, with Vibrio ranking twenty-seventh in abundance. High-throughput sequencing also established a link between previous outbreaks with increased relative abundances of Vibrio and Tenacibaculum. Therefore, monitoring the presence and abundance of these potential pathogens could be useful in providing prophylactic measures to prevent future outbreaks.

First identification and characterization ofTenacibaculum dicentrarchiisolated from Chilean red conger eel (Genypterus chilensis,Guichenot 1848)

2017 ◽  
Vol 40 (12) ◽  
pp. 1915-1920 ◽  
Author(s):  
R Irgang ◽  
R González-Luna ◽  
J Gutiérrez ◽  
M Poblete-Morales ◽  
V Rojas ◽  
...  
Keyword(s):  
Conger Eel ◽  
Identification And Characterization ◽  
Genypterus Chilensis

scholarly journals Nuclear DNA content in the red conger eel Genypterus chilensis (Guichenot, 1881) (Actinopterygii: Ophidiidae)

2011 ◽  
Vol 75 (2) ◽  
pp. 198-200 ◽  
Author(s):  
Pedro Jara-Seguel ◽  
Andrea Ubilla ◽  
Juan Estrada ◽  
Diego Ramírez ◽  
Iván Valdebenito
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Conger Eel ◽  
Genypterus Chilensis

Isolation and identification of Vibrio toranzoniae associated with diseased red conger eel (Genypterus chilensis) farmed in Chile

2015 ◽  
Vol 179 (3-4) ◽  
pp. 327-331 ◽  
Author(s):  
Aide Lasa ◽  
Ruben Avendaño-Herrera ◽  
Juan M. Estrada ◽  
Jesús L. Romalde
Keyword(s):  
Isolation And Identification ◽  
Conger Eel ◽  
Genypterus Chilensis

Temperature effect on oxidative stress and egg quality‐related genes on post‐ovulatory eggs and ovary of red cusk‐eel ( Genypterus chilensis )

2021 ◽  
Author(s):  
Phillip Dettleff ◽  
Rodrigo Zuloaga ◽  
Marcia Fuentes ◽  
Juan M. Estrada ◽  
Alfredo Molina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Temperature Effect ◽  
Egg Quality ◽  
Genypterus Chilensis

A Culture Technique Using Marine Fish Kidney to Obtain Chromosomes

1979 ◽  
Vol 36 (4) ◽  
pp. 458-461 ◽  
Author(s):  
Eun Ho Park ◽  
Sang Dai Park
Keyword(s):  
Chick Embryo ◽  
Marine Fish ◽  
Culture Technique ◽  
Kidney Cells ◽  
High Concentration ◽  
Embryo Extract ◽  
Key Words Kidney ◽  
Chick Embryo Extract ◽  
Conger Eel

A relatively simple and reliable in vitro method for marine fish chromosome study was developed. The addition of 10% chick embryo extract to serum-supplemented Eagle's minimum essential medium with high concentration of NaCl resulted in marked growth of kidney cells in the marine conger eel (Astroconger myriaster) after activation by phytohemagglutinin (PHA). Culture medium without chick embryo extract or PHA and/or with normal concentration of NaCl did not induce substantial growth. In contrast to reports by others, humidified culture was not required for excellent cell growth of these teleost kidney cells. Numerous metaphases unmarred by overlapping chromosomes were recovered and excellent karyograms were available for detailed karyotype analysis. Key words: kidney, culture, marine fish, chromosome

scholarly journals Red Cusk-Eel (Genypterus chilensis) Gut Microbiota Description of Wild and Aquaculture Specimens

2022 ◽  
Vol 10 (1) ◽  
pp. 105
Author(s):  
Jaime Romero ◽  
Osmán Díaz ◽  
Claudio D. Miranda ◽  
Rodrigo Rojas
Keyword(s):  
Intestinal Microbiota ◽  
High Throughput Sequencing ◽  
Acid Synthesis ◽  
Intestinal Content ◽  
Meat Production ◽  
Rrna Gene ◽  
Potential Contribution ◽  
Ion Torrent ◽  
The 16S Rrna Gene ◽  
Genypterus Chilensis

Chile has promoted the diversification of aquaculture and red cusk-eel (Genypterus chilensis) is one of the prioritized species. However, many aspects of the biology of the species are unknown or have little information available. These include intestinal microbiota, an element that may play an important role in the nutrition and defense of cultured animals for meat production. This study compares the microbiota composition of the intestinal contents of wild and aquaculture fish to explore the microbial communities present and their potential contribution to the host. DNA was extracted from the intestinal content samples and the V4 region of the 16S rRNA gene was amplified and sequenced using the Ion Torrent platform. After the examination of the sequences, strong differences were found in the composition at the level of phylum, being Firmicutes and Tenericutes the most abundant in aquaculture and wild condition, respectively. At the genus level, the Vagococcus (54%) and Mycoplasma (97%) were the most prevalent in the microbial community of aquaculture and wild condition, respectively. The evaluation of predicted metabolic pathways in these metagenomes showed that in wild condition there is an important presence of lipid metabolism belonging to the unsaturated fatty acid synthesis. In the aquaculture condition, the metabolism of terpenoids and polyketides were relevant. To our knowledge, this is the first study to characterize and compare the intestinal microbiota of red cusk-eel (Genypterus chilensis) of wild and aquaculture origin using high-throughput sequencing.

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