Bayesian estimation of diagnostic sensitivity and specificity of a qPCR and a bacteriological culture method for Piscirickettsia salmonis in farmed Atlantic salmon ( Salmo salar L.) in Chile

2020 ◽  
Vol 43 (10) ◽  
pp. 1167-1175 ◽  
Author(s):  
Emilie Laurin ◽  
Ian A. Gardner ◽  
Andrea Peña ◽  
Marco Rozas‐Serri ◽  
Jorge Gayosa ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Bayesian Estimation ◽  
Sensitivity And Specificity ◽  
Salmo Salar ◽  
Culture Method ◽  
Diagnostic Sensitivity ◽  
Bacteriological Culture ◽  
Piscirickettsia Salmonis ◽  
Diagnostic Sensitivity And Specificity ◽  
Farmed Atlantic Salmon

scholarly journals The Analysis of Live-Attenuated Piscirickettsia salmonis Vaccine Reveals the Short-Term Upregulation of Innate and Adaptive Immune Genes in Atlantic Salmon (Salmo salar): An In Situ Open-Sea Cages Study

2021 ◽  
Vol 9 (4) ◽  
pp. 703
Author(s):  
Deborah Vargas ◽  
Eva Vallejos-Vidal ◽  
Sebastián Reyes-Cerpa ◽  
Aarón Oyarzún-Arrau ◽  
Claudio Acuña-Castillo ◽  
...  
Keyword(s):  
Immune Response ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Cellular Response ◽  
Short Term ◽  
Live Attenuated Vaccine ◽  
Gene Markers ◽  
Post Vaccination ◽  
Atlantic Salmon Salmo Salar ◽  
Piscirickettsia Salmonis

Piscirickettsia salmonis, the etiological agent of the Salmon Rickettsial Septicemia (SRS), is one the most serious health problems for the Chilean salmon industry. Typical antimicrobial strategies used against P. salmonis include antibiotics and vaccines, but these applications have largely failed. A few years ago, the first attenuated-live vaccine against SRS (ALPHA JECT LiVac® SRS vaccine) was released to the market. However, there is no data about the agents involved in the activation of the immune response induced under field conditions. Therefore, in this study we evaluated the expression profile of a set of gene markers related to innate and adaptive immunity in the context of a cellular response in Atlantic salmon (Salmo salar) reared under productive farm conditions and immunized with a live-attenuated vaccine against P. salmonis. We analyzed the expression at zero, 5-, 15- and 45-days post-vaccination (dpv). Our results reveal that the administration of the attenuated live SRS LiVac vaccine induces a short-term upregulation of the cellular-mediated immune response at 5 dpv modulated by the upregulation of ifnα, ifnγ, and the cd4 and cd8α T cell surface markers. In addition, we also registered the upregulation of il-10 and tgfβ. Altogether, the results suggest that a balanced activation of the immune response took place only at early times post-vaccination (5 dpv). The scope of this short-term upregulation of the cellular-mediated immune response against a natural outbreak in fish subjected to productive farm conditions deserves further research.

scholarly journals Clinical significance of small molecule metabolites in the blood of patients with different types of liver injury

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hui Li ◽  
Yan Wang ◽  
Shizhao Ma ◽  
Chaoqun Zhang ◽  
Hua Liu ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Clinical Diagnosis ◽  
Sensitivity And Specificity ◽  
Acid Amide ◽  
Hbv Infection ◽  
Diagnostic Sensitivity ◽  
Future Research ◽  
Diagnostic Efficacy ◽  
Metabolic Markers ◽  
Diagnostic Sensitivity And Specificity

AbstractTo understand the characteristic of changes of serum metabolites between healthy people and patients with hepatitis B virus (HBV) infection at different stages of disease, and to provide reference metabolomics information for clinical diagnosis of liver disease patients. 255 patients with different stages of HBV infection were selected. 3 mL blood was collected from each patient in the morning to detect differences in serum lysophosphatidylcholine, acetyl-l-carnitine, oleic acid amide, and glycocholic acid concentrations by UFLC-IT-TOF/MS. The diagnostic values of four metabolic substances were evaluated by receiver operating characteristic (ROC) curve. The results showed that the optimal cut-off value of oleic acid amide concentration of the liver cirrhosis and HCC groups was 23.6 mg/L, with a diagnostic sensitivity of 88.9% and specificity of 70.6%. The diagnostic efficacies of the three substances were similar in the hepatitis and HCC groups, with an optimal cut-off value of 2.04 mg/L, and a diagnostic sensitivity and specificity of 100% and 47.2%, respectively. The optimal cut-off value of lecithin of the HBV-carrier and HCC groups was 132.85 mg/L, with a diagnostic sensitivity and specificity of 88.9% and 66.7%, respectively. The optimal cut-off value of oleic acid amide of the healthy and HCC groups was 129.03 mg/L, with a diagnostic sensitivity and specificity of 88.4% and 83.3%, respectively. Lysophosphatidylcholine, acetyl-l-carnitine, and oleic acid amide were potential metabolic markers of HCC. Among them, lysophosphatidylcholine was low in the blood of HCC patients, and its diagnostic efficacy was better than that of acetyl-l-carnitine and oleic acid amide, providing reference metabolomics information in clinical diagnosis and future research.

Movements and survival of sonically tagged farmed Atlantic salmon released in Cobscook Bay, Maine, USA

2006 ◽  
Vol 63 (7) ◽  
pp. 1218-1223 ◽  
Author(s):  
Frederick G. Whoriskey ◽  
Paul Brooking ◽  
Gino Doucette ◽  
Stephen Tinker ◽  
Jonathan W. Carr
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Coastal Zone ◽  
Coastal Region ◽  
Movement Patterns ◽  
Tidal Currents ◽  
Bay Of Fundy ◽  
Wild Salmon ◽  
Receiver Array ◽  
Farmed Atlantic Salmon

Abstract We sonically tagged and released farmed Atlantic salmon (Salmo salar) from a cage site in Cobscook Bay, Maine, USA. The fish were released in January (n = 75) and in April and May (n = 198) 2004 to study their movement patterns and survival and to assess the possibility of recapturing them. Inshore and offshore waters in this region are subject to intense tidal currents. Tagged salmon dispersed >1 km from the cage site within a few hours of their release. Mortality was high within Cobscook Bay and the surrounding coastal region (56% of the winter (January) releases; 84% of the spring (March) releases), probably the result of seal predation. Most surviving fish exited the coastal zone and entered the Bay of Fundy along the routes of the dominant tidal currents, passing through Canadian waters. No tagged fish were detected during the wild salmon spawning season in autumn 2004 in any of the 43 monitored salmon rivers draining into the Bay of Fundy, or during 2005 either in the Magaguadavic River, the site of the hatchery in which the fish were reared to the smolt stage, or by a limited coastal receiver array.

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