Availability of culture filtrate protein‐10 (CFP‐10) secreted from Mycobacterium pseudoshottsii for mycobacteriosis diagnosis in ginbuna crucian carp Carrasius auratus langsdorfii

2019 ◽  
Vol 43 (1) ◽  
pp. 81-89
Author(s):  
Megumi Matsumoto ◽  
Kazuma Hayashi ◽  
Kyosuke Araki ◽  
Teruyuki Nakanishi ◽  
Atsushi Yamamoto
Keyword(s):  
Culture Filtrate ◽  
Crucian Carp ◽  
Culture Filtrate Protein ◽  
Ginbuna Crucian Carp

scholarly journals Vaccine-Elicited 10-Kilodalton Culture Filtrate Protein-Specific CD8+ T Cells Are Sufficient To Mediate Protection against Mycobacterium tuberculosis Infection

2008 ◽  
Vol 76 (5) ◽  
pp. 2249-2255 ◽  
Author(s):  
Ying Wu ◽  
Joshua S. Woodworth ◽  
Daniel S. Shin ◽  
Sheldon Morris ◽  
Samuel M. Behar
Keyword(s):  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Culture Filtrate ◽  
Protective Immunity ◽  
Protective Antigen ◽  
T Cell Response ◽  
Rapid Expansion ◽  
Mycobacterium Tuberculosis Infection ◽  
Infected People ◽  
Culture Filtrate Protein

ABSTRACT The 10-kDa culture filtrate protein (CFP-10) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Mycobacterium bovis bacillus Calmette-Guérin (BCG), and it is hypothesized that these proteins are important targets of protective immunity. Indeed, vaccination with ESAT-6 elicits protective CD4+ T cells in C57BL/6 mice. We have previously shown that M. tuberculosis infection of C3H mice elicits CFP-10-specific CD8+ and CD4+ T cells. Here we demonstrate that immunization with a CFP-10 DNA vaccine stimulates a specific T-cell response only to the H-2Kk-restricted epitope CFP-1032-39. These CFP-1032-39-specific CD8+ cells undergo a rapid expansion and accumulate in the lung following challenge of immunized mice with aerosolized M. tuberculosis. Protective immunity is induced by CFP-10 DNA vaccination as measured by a CFU reduction in the lung and spleen 4 and 8 weeks after challenge with M. tuberculosis. These data demonstrate that CFP-10 is a protective antigen and that CFP-1032-39-specific CD8+ T cells elicited by vaccination are sufficient to mediate protection against tuberculosis.

scholarly journals Comparative Analysis of B- and T-Cell Epitopes of Mycobacterium leprae and Mycobacterium tuberculosis Culture Filtrate Protein 10

2004 ◽  
Vol 72 (6) ◽  
pp. 3161-3170 ◽  
Author(s):  
John S. Spencer ◽  
Hee Jin Kim ◽  
Angela M. Marques ◽  
Mercedes Gonzalez-Juarerro ◽  
Monica C. B. S. Lima ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Cell ◽  
Culture Filtrate ◽  
Mycobacterium Leprae ◽  
Cross Reactivity ◽  
Interferon Response ◽  
Positive Staining ◽  
T Cell Epitopes ◽  
Peptide Epitopes ◽  
Culture Filtrate Protein

ABSTRACT Culture filtrate protein 10 (CFP-10) from Mycobacterium tuberculosis is a well-characterized immunodominant 10-kDa protein antigen known to elicit a very potent early gamma interferon response in T cells from M. tuberculosis-infected mice and humans. The sequence of the Mycobacterium leprae homologue of CFP-10 shows only 40% identity (60% homology) at the protein level with M. tuberculosis CFP-10 and thus has the potential for development as a T- or B-cell reactive antigen for specific diagnosis of leprosy. Antisera raised in mice or rabbits against recombinant M. leprae and M. tuberculosis CFP-10 proteins reacted only with homologous peptides from arrays of overlapping synthetic peptides, indicating that there was no detectable cross-reactivity at the antibody level. Sera from leprosy and tuberculosis patients were also specific for the homologous protein or peptides and showed distinct patterns of recognition for either M. leprae or M. tuberculosis CFP-10 peptides. At the cellular level, only 2 of 45 mouse T-cell hybridomas raised against either M. leprae or M. tuberculosis CFP-10 displayed a cross-reactive response against the N-terminal heterologous CFP-10 peptide, the region that exhibits the highest level of identity in the two proteins; however, the majority of peptide epitopes recognized by mouse T-cell hybridomas specific for each protein did not cross-react with heterologous peptides. Coupled with the human serology data, these results raise the possibility that peptides that could be used to differentiate infections caused by these two related microorganisms could be developed. Immunohistochemical staining of sections of M. leprae-infected nude mouse footpads resulted in strongly positive staining in macrophages and dendritic cells, as well as weaker staining in extracellular areas, suggesting that M. leprae CFP-10, like its homologue in M. tuberculosis, is a secreted protein.

scholarly journals Generation of virus-specific CD8+ T cells by vaccination with inactivated virus in the intestine of ginbuna crucian carp

2019 ◽  
Vol 93 ◽  
pp. 37-44 ◽  
Author(s):  
Seisuke Tajimi ◽  
Masakazu Kondo ◽  
Teruyuki Nakanishi ◽  
Takahiro Nagasawa ◽  
Miki Nakao ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Crucian Carp ◽  
Ginbuna Crucian Carp

scholarly journals Percentage Dynamics of CD4-1+ and CD8α+ T Cells in Different Tissues at Various Developmental Stages in Ginbuna Crucian Carp Carassius auratus langsdorfii

2018 ◽  
Vol 53 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Mohamed A. Khallaf ◽  
Shino Ogawa ◽  
Hideaki Toda ◽  
Yuta Matsuura ◽  
Ryuichiro Miyazawa ◽  
...  
Keyword(s):  
T Cells ◽  
Carassius Auratus ◽  
Crucian Carp ◽  
Developmental Stages ◽  
Different Tissues ◽  
Ginbuna Crucian Carp

Peculiar monomeric interferon gammas, IFNγrel 1 and IFNγrel 2, in ginbuna crucian carp

FEBS Journal ◽  
2013 ◽  
Vol 281 (4) ◽  
pp. 1046-1056 ◽  
Author(s):  
Yasuhiro Shibasaki ◽  
Takeshi Yabu ◽  
Kyosuke Araki ◽  
Nobuhiro Mano ◽  
Hajime Shiba ◽  
...  
Keyword(s):  
Crucian Carp ◽  
Ginbuna Crucian Carp

scholarly journals Detection of Active Tuberculosis Infection by T Cell Responses to Early‐Secreted Antigenic Target 6‐kDa Protein and Culture Filtrate Protein 10

2000 ◽  
Vol 181 (5) ◽  
pp. 1850-1854 ◽  
Author(s):  
Sandra M. Arend ◽  
Peter Andersen ◽  
Krista E. van Meijgaarden ◽  
Rikke L. V. Skjøt ◽  
Yanri W. Subronto ◽  
...  
Keyword(s):  
T Cell ◽  
Culture Filtrate ◽  
T Cell Responses ◽  
Active Tuberculosis ◽  
Tuberculosis Infection ◽  
Cell Responses ◽  
Culture Filtrate Protein

scholarly journals Antigenic Equivalence of Human T-Cell Responses toMycobacterium tuberculosis-Specific RD1-Encoded Protein Antigens ESAT-6 and Culture Filtrate Protein 10 and to Mixtures of Synthetic Peptides

2000 ◽  
Vol 68 (6) ◽  
pp. 3314-3321 ◽  
Author(s):  
Sandra M. Arend ◽  
Annemieke Geluk ◽  
Krista E. van Meijgaarden ◽  
Jaap T. van Dissel ◽  
Michael Theisen ◽  
...  
Keyword(s):  
T Cell ◽  
Culture Filtrate ◽  
Large Scale ◽  
Mononuclear Cells ◽  
Genomic Region ◽  
Protein Antigens ◽  
Peripheral Blood Mononuclear ◽  
Human T Cell ◽  
Peptide Mixtures ◽  
Culture Filtrate Protein

ABSTRACT The early secreted antigenic target 6-kDa protein (ESAT-6) and culture filtrate protein 10 (CFP-10) are promising antigens for reliable immunodiagnosis of tuberculosis. Both antigens are encoded by RD1, a genomic region present in all strains of Mycobacterium tuberculosis and M. bovis but lacking in all M. bovis bacillus Calmette-Guérin vaccine strains. Production and purification of recombinant antigens are laborious and costly, precluding rapid and large-scale testing. Aiming to develop alternative diagnostic reagents, we have investigated whether recombinant ESAT-6 (rESAT-6) and recombinant CFP-10 (rCFP-10) can be replaced with corresponding mixtures of overlapping peptides spanning the complete amino acid sequence of each antigen. Proliferation of M. tuberculosis-specific human T-cell lines in response to rESAT-6 and rCFP-10 and that in response to the corresponding peptide mixtures were almost completely correlated (r = 0.96,P < 0.0001 for ESAT-6; r = 0.98,P < 0.0001 for CFP-10). More importantly, the same was found when gamma interferon production by peripheral blood mononuclear cells in response to these stimuli was analyzed (r = 0.89, P < 0.0001 for ESAT-6;r = 0.89, P < 0.0001 for CFP-10). Whole protein antigens and the peptide mixtures resulted in identical sensitivity and specificity for detection of infection with M. tuberculosis. The peptides in each mixture contributing to the overall response varied between individuals with different HLA-DR types. Interestingly, responses to CFP-10 were significantly higher in the presence of HLA-DR15, which is the major subtype of DR2. These results show that mixtures of synthetic overlapping peptides have potency equivalent to that of whole ESAT-6 and CFP-10 for sensitive and specific detection of infection with M. tuberculosis, and peptides have the advantage of faster production at lower cost.

Lack of a contact requirement for direct antibacterial activity of lymphocyte subpopulations in ginbuna crucian carp

2014 ◽  
Vol 39 (2) ◽  
pp. 178-184 ◽  
Author(s):  
Haitham M. Tartor ◽  
Yuta Matsuura ◽  
Gamal El-Nobi ◽  
Teruyuki Nakanishi
Keyword(s):  
Antibacterial Activity ◽  
Crucian Carp ◽  
Lymphocyte Subpopulations ◽  
Ginbuna Crucian Carp

Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii

2008 ◽  
Vol 74 (2) ◽  
pp. 341-346 ◽  
Author(s):  
Seiko NONAKA ◽  
Tomonori SOMAMOTO ◽  
Yoko KATO-UNOKI ◽  
Mitsuru OTOTAKE ◽  
Teruyuki NAKANISHI ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Carassius Auratus ◽  
Crucian Carp ◽  
Ginbuna Crucian Carp ◽  
Crucian Carp Carassius Auratus
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