The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses

2019 ◽  
Vol 42 (5) ◽  
pp. 703-712 ◽  
Author(s):  
Lixing Huang ◽  
Lina Guo ◽  
Xiaojin Xu ◽  
Yingxue Qin ◽  
Lingmin Zhao ◽  
...  
Keyword(s):  
Vibrio Alginolyticus

Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus

2011 ◽  
Vol 101 (2) ◽  
pp. 281-288 ◽  
Author(s):  
Chen Chang ◽  
Wang Qing-bai ◽  
Liu Zhu-Hong ◽  
Zhao Jing-jing ◽  
Jiang Xiao ◽  
...  
Keyword(s):  
Vibrio Alginolyticus ◽  
Toxr Gene

scholarly journals Expression analysis and tissue localization of IgZ in the grouper Epinephelus coioides after Vibrio alginolyticus infection and vaccination

2021 ◽  
Author(s):  
Yao-Wu Fu ◽  
Zhan-Juan Yao ◽  
Miao Cui ◽  
Qi-Zhong Zhang
Keyword(s):  
Immune Responses ◽  
Bacterial Pathogen ◽  
Head Kidney ◽  
Vibrio Alginolyticus ◽  
Economic Losses ◽  
Epinephelus Coioides ◽  
Farmed Fish ◽  
Transcriptional Changes

Abstract Background The orange-spotted grouper (Epinephelus coioides) is an important marine farmed fish in China. It is affected by the bacterial pathogen Vibrio alginolyticus, which causes high mortality and substantial economic losses. Results We studied the transcriptional changes of the IgZ gene in E. coioides following V. alginolyticus stimulation and investigated the distribution of IgZ in different tissues. The highest expression level of IgZ occurred in the head kidney. When fish were exposed to live and inactivated V. alginolyticus, the expression levels of IgZ in head kidney, spleen, intestine, gill, and blood cell were significantly upregulated. In an in situ hybridization study, IgZ mRNA-positive cells were detected in head kidney, spleen, and gill, but positive signals were not detected in liver and intestine. IgZ-labeled cells increased in the head kidney, spleen, and gill post-infection with V. alginolyticus for 21 days. Conclusions The present study provided additional evidence that IgZ was involved in mucosal immune responses against pathogens. These results help explain the role of IgZ in E. coioides defense against V. alginolyticus infection.

The role of dctP gene in regulating colonization, adhesion and pathogenicity of Vibrio alginolyticus strain HY9901

2021 ◽  
Author(s):  
Yilin Zhang ◽  
Huimin Tan ◽  
Shiping Yang ◽  
Yucong Huang ◽  
Shuanghu Cai ◽  
...  
Keyword(s):  
Vibrio Alginolyticus

Role of RpoS in stress survival, synthesis of extracellular autoinducer 2, and virulence in Vibrio alginolyticus

2008 ◽  
Vol 190 (5) ◽  
pp. 585-594 ◽  
Author(s):  
Yang Tian ◽  
Qiyao Wang ◽  
Qin Liu ◽  
Yue Ma ◽  
Xiaodan Cao ◽  
...  
Keyword(s):  
Vibrio Alginolyticus ◽  
Autoinducer 2 ◽  
Stress Survival

scholarly journals 3P196 Dissection of role of MotY in the Na^+-driven flagellar rotation of Vibrio alginolyticus

2005 ◽  
Vol 45 (supplement) ◽  
pp. S252
Author(s):  
J. Yagasaki ◽  
M. Okabe ◽  
R. Kurebayashi ◽  
T. Yakushi ◽  
M. Homma
Keyword(s):  
Vibrio Alginolyticus ◽  
Flagellar Rotation

scholarly journals First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence

2021 ◽  
Vol 10 ◽  
Author(s):  
Fuyuan Zeng ◽  
Huanying Pang ◽  
Ying Chen ◽  
Hongwei Zheng ◽  
Wanxin Li ◽  
...  
Keyword(s):  
Tca Cycle ◽  
Vibrio Alginolyticus ◽  
Post Translational Modification ◽  
Systematic Analysis ◽  
Cellular Processes ◽  
Affinity Enrichment ◽  
Lysine Succinylation ◽  
Sec System ◽  
Serious Disease

Recent studies have shown that a key strategy of many pathogens is to use post-translational modification (PTMs) to modulate host factors critical for infection. Lysine succinylation (Ksuc) is a major PTM widespread in prokaryotic and eukaryotic cells, and is associated with the regulation of numerous important cellular processes. Vibrio alginolyticus is a common pathogen that causes serious disease problems in aquaculture. Here we used the affinity enrichment method with LC-MS/MS to report the first identification of 2082 lysine succinylation sites on 671 proteins in V. alginolyticus, and compared this with the lysine acetylation of V. alginolyticus in our previous work. The Ksuc modification of SodB and PEPCK proteins were further validated by Co-immunoprecipitation combined with Western blotting. Bioinformatics analysis showed that the identified lysine succinylated proteins are involved in various biological processes and central metabolism pathways. Moreover, a total of 1,005 (25.4%) succinyl sites on 502 (37.3%) proteins were also found to be acetylated, which indicated that an extensive crosstalk between acetylation and succinylation in V. alginolyticus occurs, especially in three central metabolic pathways: glycolysis/gluconeogenesis, TCA cycle, and pyruvate metabolism. Furthermore, we found at least 50 (7.45%) succinylated virulence factors, including LuxS, Tdh, SodB, PEPCK, ClpP, and the Sec system to play an important role in bacterial virulence. Taken together, this systematic analysis provides a basis for further study on the pathophysiological role of lysine succinylation in V. alginolyticus and provides targets for the development of attenuated vaccines.

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