In vivo virulence and genomic comparison of infectious Salmon Anaemia Virus isolates from Atlantic Canada

2018 ◽  
Vol 41 (9) ◽  
pp. 1373-1384 ◽  
Author(s):  
Francis LeBlanc ◽  
Steven Leadbeater ◽  
Mark Laflamme ◽  
Nellie Gagné
Keyword(s):  
Atlantic Canada ◽  
Infectious Salmon Anaemia Virus ◽  
Genomic Comparison ◽  
Virus Isolates ◽  
Infectious Salmon Anaemia

scholarly journals Modelling the spread of infectious salmon anaemia among salmon farms based on seaway distances between farms and genetic relationships between infectious salmon anaemia virus isolates

2011 ◽  
Vol 8 (62) ◽  
pp. 1346-1356 ◽  
Author(s):  
M. Aldrin ◽  
T. M. Lyngstad ◽  
A. B. Kristoffersen ◽  
B. Storvik ◽  
Ø. Borgan ◽  
...  
Keyword(s):  
Genetic Relationships ◽  
Genetic Distances ◽  
Infectious Salmon Anaemia Virus ◽  
Time Data ◽  
Farmed Salmon ◽  
Source Of Infection ◽  
History Of ◽  
Isa Virus ◽  
Virus Isolates ◽  
Infectious Salmon Anaemia

Infectious salmon anaemia (ISA) is an important infectious disease in Atlantic salmon farming causing recurrent epidemic outbreaks worldwide. The focus of this paper is on tracing the spread of ISA among Norwegian salmon farms. To trace transmission pathways for the ISA virus (ISAV), we use phylogenetic relationships between virus isolates in combination with space–time data on disease occurrences. The rate of ISA infection of salmon farms is modelled stochastically, where seaway distances between farms and genetic distances between ISAV isolates from infected farms play prominent roles. The model was fitted to data covering all cohorts of farmed salmon and the history of all farms with ISA between 2003 and summer 2009. Both seaway and genetic distances were significantly associated with the rate of ISA infection. The fitted model predicts that the risk of infection from a neighbourhood infectious farm decreases with increasing seaway distance between the two farms. Furthermore, for a given infected farm with a given ISAV genotype, the source of infection is significantly more likely to be ISAV of a small genetic distance than of moderate or large genetic distances. Nearly half of the farms with ISA in the investigated period are predicted to have been infected by an infectious farm in their neighbourhood, whereas the remaining half of the infected farms had unknown sources. For many of the neighbourhood infected farms, it was possible to point out one or a few infectious farms as the most probable sources of infection. This makes it possible to map probable infection pathways.

Comparative virulence of Infectious salmon anaemia virus isolates in Atlantic salmon,Salmo salarL.

2009 ◽  
Vol 32 (2) ◽  
pp. 157-171 ◽  
Author(s):  
R J Ritchie ◽  
J T McDonald ◽  
B Glebe ◽  
W Young-Lai ◽  
E Johnsen ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Infectious Salmon Anaemia Virus ◽  
Virus Isolates ◽  
Infectious Salmon Anaemia

scholarly journals Antigenic variation among isolates of infectious salmon anaemia virus correlates with genetic variation of the viral haemagglutinin gene

2001 ◽  
Vol 82 (12) ◽  
pp. 2869-2879 ◽  
Author(s):  
Frederick S. B. Kibenge ◽  
Molly J. T. Kibenge ◽  
Patricia K. McKenna ◽  
Paul Stothard ◽  
Rebecca Marshall ◽  
...  
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Amino Acid Sequence ◽  
Antigenic Variation ◽  
Comparative Sequence Analysis ◽  
Infectious Salmon Anaemia Virus ◽  
Marine Aquaculture ◽  
Sequence Identity ◽  
Ha Gene ◽  
Infectious Salmon Anaemia

Infectious salmon anaemia virus (ISAV), an orthomyxovirus-like virus, is an important fish pathogen in marine aquaculture. Virus neutralization of 24 ISAV isolates in the TO cell line using rabbit antisera to the whole virus and comparative sequence analysis of their haemagglutinin (HA) genes have allowed elaboration on the variation of ISAV isolates. The 24 viruses were neutralized to varying degrees, revealing two major antigenic groups, one American and one European. Sequence analysis of the HA gene also revealed two groups of viruses (genotypes) that correlated with the antigenic groupings. The two HA subtypes had nucleotide sequence identity of only ⩽79·4% and amino acid sequence identity of ⩽84·5% whereas, within each subtype, the sequence identities were 90·7% or higher. This grouping was also evident upon phylogenetic analysis, which revealed two distinct phylogenetic families. Between the two groups, the amino acid sequence was most variable in the C-terminal region and included deletions of 4–16 amino acids in all isolates relative to ISAV isolate RPC/NB-980 280-2. In order to view the relationships among these sequences and the HA sequences of the established orthomyxoviruses, a second phylogenetic tree was constructed which showed the ISAV sequences to be more closely related to sequences from Influenzavirus A and Influenzavirus B than to sequences from Influenzavirus C and Thogotovirus. The extensive deletions in the gene of European ISAV isolates lead us to speculate that the archetypal ISAV was probably of Canadian origin.

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