A survey of microparasites present in adult migrating Chinook salmon (Oncorhynchus tshawytscha) in south-western British Columbia determined by high-throughput quantitative polymerase chain reaction

2017 ◽  
Vol 40 (4) ◽  
pp. 453-477 ◽  
Author(s):  
A L Bass ◽  
S G Hinch ◽  
A K Teffer ◽  
D A Patterson ◽  
K M Miller
Keyword(s):  
British Columbia ◽  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals High-throughput applicable genomic sex typing of chicken by TaqMan real-time quantitative polymerase chain reaction

2010 ◽  
Vol 89 (7) ◽  
pp. 1451-1456 ◽  
Author(s):  
N.F. Rosenthal ◽  
H. Ellis ◽  
K. Shioda ◽  
C. Mahoney ◽  
K.R. Coser ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Sex Typing ◽  
Polymerase Chain

scholarly journals Development of a High-Throughput Method for Quantification of Plasmopara viticola DNA in Grapevine Leaves by Means of Quantitative Real-Time Polymerase Chain Reaction

2005 ◽  
Vol 95 (6) ◽  
pp. 672-678 ◽  
Author(s):  
G. Valsesia ◽  
D. Gobbin ◽  
A. Patocchi ◽  
A. Vecchione ◽  
I. Pertot ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Fluorescent Dyes ◽  
Control Strategies ◽  
Quantitative Polymerase Chain Reaction ◽  
Plasmopara Viticola ◽  
Chain Reaction ◽  
High Throughput Method ◽  
Polymerase Chain

Plasmopara viticola is a strictly biotrophic oomycete that causes downy mildew, which is one of the most important grapevine diseases. Control of the disease is most often achieved by fungicide applications, which may have severe environmental consequences. Therefore, alternative control strategies based on biocontrol agents (BCAs) are currently in development. Thousands of potential BCAs have to be screened for their antagonist efficacy against Plasmopara viticola. Evaluation of their effect on the pathogen can be achieved by detecting the amount of P. viticola DNA in leaves treated with potential antagonists and infected with the pathogen. In this study, a rapid high-throughput method was developed for relative quantification of P. viticola DNA directly from Vitis vinifera leaves by means of multiplex real-time quantitative polymerase chain reaction (PCR) with TaqMan chemistry. This method allows simultaneous amplification, but independent detection, of pathogen and host DNA by using species-specific primers and TaqMan probes that are labeled with different fluorescent dyes. Including detection of V. vinifera DNA in the tests is fundamental because it provides an endogenous reference and allows normalization for variations caused by sample-to-sample differences in DNA extraction, PCR efficiencies, and pipetting volumes. The developed method allows highly sensitive and specific detection of P. viticola DNA (minimal detectable quantity of 0.1 pg). Moreover, high precision and reproducibility of TaqMan assays were observed over a linear range of four orders of magnitude, confirming the reliability of the developed PCR assay. Potential applications range from screening for BCA efficiency to evaluation of fungicide efficacy, or assessment of host resistance.

Mitochondrial DNA Variation in Chinook (Oncorhynchus tshawytscha) and Chum Salmon (O. keta) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products

1993 ◽  
Vol 50 (4) ◽  
pp. 708-715 ◽  
Author(s):  
Matthew A. Cronin ◽  
William J. Spearman ◽  
Richard L. Wilmot ◽  
John C. Patton ◽  
John W. Bickham
Keyword(s):  
Polymerase Chain Reaction ◽  
Mitochondrial Dna ◽  
Chinook Salmon ◽  
Chum Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Vancouver Island ◽  
Chain Reaction ◽  
Dna Variation ◽  
Yukon River ◽  
Polymerase Chain

We analyzed intraspecific mitochondrial DNA variation in chinook salmon (Oncorhynchus tshawytscha) from drainages in the Yukon River (Alaska and Yukon Territory), the Kenai River (Alaska), and Oregon and California rivers; and chum salmon (O. keta) from the Yukon River and Vancouver Island, and Washington rivers. For each species, three different portions of the mtDNA molecule were amplified separately using the polymerase chain reaction and then digested with at least 19 restriction enzymes. Intraspecific sequence divergences between haplotypes were less than 0.01 base substitution per nucleotide. Nine chum salmon haplotypes were identified. Yukon River chum salmon stocks displayed more haplotypes (eight) than the stocks of Vancouver Island and Washington (two). The most common chum salmon haplotype occurred in all areas. Seven chinook salmon haplotypes were identified. Four haplotypes occurred in the Yukon and Kenai rivers and four occurred in Oregon/California, with only one haplotype shared between the regions. Sample sizes were too small to quantify the degree of stock separation among drainages, but the patterns of variation that we observed suggest utility of the technique in genetic stock identification.

scholarly journals High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets

2008 ◽  
Vol 80 (23) ◽  
pp. 8975-8981 ◽  
Author(s):  
Margaret Macris Kiss ◽  
Lori Ortoleva-Donnelly ◽  
N. Reginald Beer ◽  
Jason Warner ◽  
Christopher G. Bailey ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain
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