In vivo study of viral haemorrhagic septicaemia virus and infectious pancreatic necrosis virus coexistence in Senegalese sole (Solea senegalensis )

2016 ◽  
Vol 40 (9) ◽  
pp. 1129-1139 ◽  
Author(s):  
C López-Vázquez ◽  
M C Alonso ◽  
C P Dopazo ◽  
I Bandín
Keyword(s):  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Solea Senegalensis ◽  
Senegalese Sole ◽  
Necrosis Virus ◽  
Haemorrhagic Septicaemia ◽  
Viral Haemorrhagic Septicaemia Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

scholarly journals Infectious Pancreatic Necrosis Virus VP5 Is Dispensable for Virulence and Persistence

2005 ◽  
Vol 79 (14) ◽  
pp. 9206-9216 ◽  
Author(s):  
Nina Santi ◽  
Haichen Song ◽  
Vikram N. Vakharia ◽  
Øystein Evensen
Keyword(s):  
Atlantic Salmon ◽  
Pancreatic Necrosis ◽  
Stop Codon ◽  
Premature Stop Codon ◽  
Infectious Pancreatic Necrosis Virus ◽  
Nucleotide Position ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

ABSTRACT Infectious pancreatic necrosis virus (IPNV) is the causative agent of infectious pancreatic necrosis (IPN) disease in salmonid fish. Recent studies have revealed variation in virulence between isolates of the Sp serotype, associated with certain residues of the structural protein VP2. The isolates are also highly heterogenic in the coding region of the nonstructural VP5 protein. To study the involvement of this protein in the pathogenesis of disease, we generated three recombinant VP5 mutant viruses using reverse genetics. The “wild-type” recombinant NVI15 (rNVI15) virus is virulent, having a premature stop codon at nucleotide position 427, putatively encoding a truncated 12-kDa VP5 protein, whereas rNVI15-15K virus encodes a 15-kDa protein. Recombinant rNVI15-ΔVP5 virus contains a mutation in the initiation codon of the VP5 gene that ablates the expression of VP5. Atlantic salmon postsmolts were challenged to study the virulence characteristics of the recovered viruses in vivo. The role of VP5 in persistent infection was investigated by challenging Atlantic salmon fry with the recovered viruses, as well as with the low-virulence field strain Sp103 and a naturally occurring VP5-deficient mutant of Sp103. The results show that VP5 is not required for viral replication in vivo, and its absence does not alter the virulence characteristics of the virus or the establishment of persistent IPNV infection.

scholarly journals The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

Pathogens ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 94 ◽  
Author(s):  
Carlos P. Dopazo
Keyword(s):  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
General Term ◽  
Virulence Determinants ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Viral Components

Infectious pancreatic necrosis (IPN) is a disease of great concern in aquaculture, mainly among salmonid farmers, since losses in salmonid fish—mostly very young rainbow trout (Salmo gairdnery) fry and Atlantic salmon (Salmo salar) post-smolt—frequently reach 80–90% of stocks. The virus causing the typical signs of the IPN disease in salmonids, named infectious pancreatic necrosis virus (IPNV), has also been isolated from other fish species either suffering related diseases (then named IPNV-like virus) or asymptomatic; the general term aquabirnavirus is used to encompass all these viruses. Aquabirnaviruses are non-enveloped, icosahedral bisegmented dsRNA viruses, whose genome codifies five viral proteins, three of which are structural, and one of them is an RNA-dependent RNA polymerase. Due to the great importance of the disease, there have been great efforts to find a way to predict the level of virulence of IPNV isolates. The viral genome and proteins have been the main focus of research. However, to date such a reliable magic marker has not been discovered. This review describes the processes followed for decades in the attempts to discover the viral determinants of virulence, and to help the reader understand how viral components can be involved in virulence modulation in vitro and in vivo. There is also a brief description of the disease, of host defenses, and of the molecular structure and function of the virus and its viral components.

scholarly journals Infectious pancreatic necrosis virus induces apoptosis in vitro and in vivo independent of VP5 expression

Virology ◽  
2005 ◽  
Vol 342 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Nina Santi ◽  
Ane Sandtrø ◽  
Hilde Sindre ◽  
Haichen Song ◽  
Jiann-Ruey Hong ◽  
...  
Keyword(s):  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus
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