Pathology of two forms of shell disease of the American lobster Homarus americanus Milne Edwards in Atlantic Canada

2013 ◽  
Vol 37 (6) ◽  
pp. 577-581 ◽  
Author(s):  
R Smolowitz ◽  
R A Quinn ◽  
R J Cawthorn ◽  
R L Summerfield ◽  
A Y Chistoserdov
Keyword(s):  
Homarus Americanus ◽  
American Lobster ◽  
Atlantic Canada ◽  
Shell Disease

Bacterial communities associated with lesions of two forms of shell disease in the American lobster (Homarus americanus, Milne Edwards) from Atlantic Canada

2013 ◽  
Vol 59 (6) ◽  
pp. 380-390 ◽  
Author(s):  
Robert A. Quinn ◽  
Richard J. Cawthorn ◽  
Rachael L. Summerfield ◽  
Roxanna Smolowitz ◽  
Andrei Y. Chistoserdov
Keyword(s):  
Bacterial Communities ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Homarus Americanus ◽  
American Lobster ◽  
Atlantic Canada ◽  
Yellow Color ◽  
Gradient Gel Electrophoresis ◽  
Shell Disease ◽  
Polymerase Chain ◽  
Vibrio Spp

Shell disease is a major threat to the American lobster (Homarus americanus, Milne Edwards) fishery. Here we describe the composition of microbial communities associated with lesions of 2 forms of shell disease in Atlantic Canada, (i) a trauma shell disease (TSD) characterized by massive lesions and (ii) an enzootic shell disease (EnSD) characterized by irregularly shaped lesions with a distinct orange to yellow color. The microbiology of the lesions was described by polymerase chain reaction and denaturing gradient gel electrophoresis of 16S rDNA amplified from scrapings of the shell lesions and was compared with communities of unaffected carapaces and previously described forms of shell diseases. Both TSD and EnSD lesions were dominated by members of Alphaproteobacteria, Gammaproteobacteria, and Flavobacteria, all commonly detected in other forms of shell disease; however, unique members of Epsilonproteobacteria were also present. Two Vibrio spp. and 2 Pseudoalteromonas spp. were dominant in lesions of TSD and a Tenacibaculum sp. and Tenacibaculum ovolyticum were dominant in lesions of EnSD. The TSD and EnSD in this study contained similar taxa as other shell disease forms; however, their microbiology is mostly different and neither resembles that of epizootic shell disease.

scholarly journals Draft Genome Sequence of the Putative Marine Pathogen Aquimarina sp. Strain I32.4

2018 ◽  
Vol 6 (17) ◽  
Author(s):  
Hilary J. Ranson ◽  
Jason LaPorte ◽  
Edward Spinard ◽  
Andrei Y. Chistoserdov ◽  
Marta Gomez-Chiarri ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Genome Sequence ◽  
Draft Genome ◽  
Homarus Americanus ◽  
Draft Genome Sequence ◽  
American Lobster ◽  
Content Type ◽  
Shell Disease ◽  
Insight Into

ABSTRACT Aquimarina sp. strain I32.4 (formerly Aquimarina sp. ‘ homaria ’) is a putative pathogen involved in epizootic shell disease in the American lobster ( Homarus americanus ). We report here the draft genome sequence for Aquimarina sp. strain I32.4 and describe virulence factors that may provide insight into its mechanism of pathogenicity.

scholarly journals Newly Hatched Stage I American Lobster (Homarus americanus) Survival Following Exposure to Physically and Chemically Dispersed Crude Oil

2021 ◽  
Author(s):  
Benjamin de Jourdan ◽  
Tahereh Boloori ◽  
Les Burridge
Keyword(s):  
Environmental Protection Agency ◽  
Native Species ◽  
Toxicity Testing ◽  
Homarus Americanus ◽  
Larval Survival ◽  
Stage I ◽  
Toxicity Tests ◽  
American Lobster ◽  
Atlantic Canada ◽  
Test Species

Abstract Standard model species are commonly used in toxicity tests due to their biological and technical advantages but studying native species increases the specificity and relevance of results generated for the potential risk assessment to an ecosystem. Accounting for intraspecies variability and other factors, such as chemical and physical characterization of test medium, is necessary to develop a reproducible bioassay for toxicity testing with native species. In this study, larval stage I American lobster (Homarus americanus) was selected as the test species, which is native to Atlantic Canada. Toxicity tests were first conducted exposing lobster larvae to a reference toxicant of copper sulfate (CuSO4) and then to physically and chemically dispersed oil. The effect on larval survival was estimated by calculating the median effect concentration (EC50) as 2.54-9.73 mg TPH/L when all trials are considered together. The HC5 or PNEC value was 2.52 mg TPH/L and therefore a narrow difference from the EC50 value. The inter-trial variability (coefficient of variability = 17%) was lower than the US Environmental Protection Agency standard test species of mysid shrimp (Americamysis bahia) and inland silversides (Menidia bervillina). Our results indicate that the described larval lobster bioassay is reliable to produce repeatable results for this commercially important and native species of Atlantic Canada.

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