Acai berry extract as a regulator of intestinal inflammation pathways in a Caco‐2 and RAW 264.7 co‐culture model

2021 ◽  
Author(s):  
Kyeong Jin Kim ◽  
Yunyoung Kim ◽  
Sul Gi Jin ◽  
Ji Yeon Kim
Keyword(s):  
Intestinal Inflammation ◽  
Raw 264.7 ◽  
Culture Model ◽  
Acai Berry

Standardization of a human organ culture model of intestinal inflammation and its application for drug testing

2015 ◽  
Vol 421 ◽  
pp. 96-103 ◽  
Author(s):  
Timea Szikszai ◽  
Felix Lasitschka ◽  
Thomas Giese ◽  
Matthias Greulich ◽  
Young-Seon Lee ◽  
...  
Keyword(s):  
Organ Culture ◽  
Drug Testing ◽  
Intestinal Inflammation ◽  
Human Organ ◽  
Culture Model

scholarly journals Administration of Protein Kinase D1 Induces a Protective Effect on Lipopolysaccharide-Induced Intestinal Inflammation in a Co-Culture Model of Intestinal Epithelial Caco-2 Cells and RAW264.7 Macrophage Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Ditte Søvsø Gundelund Nielsen ◽  
Marlene Fredborg ◽  
Vibeke Andersen ◽  
Stig Purup
Keyword(s):  
Protein Kinase ◽  
Inflammatory Response ◽  
Protective Effect ◽  
Intestinal Inflammation ◽  
Raw264.7 Cells ◽  
Intestinal Epithelial ◽  
Necrosis Factor Alpha ◽  
Protein Kinase D1 ◽  
Raw264.7 Macrophages ◽  
Culture Model

Inflammatory bowel diseases (IBD) are chronic inflammatory diseases involving all or part of the gastrointestinal tract. The stress-activated serine-threonine protein kinase D1 (PKD1) protein has previously been implicated in intestinal immune regulation. The objective of this study was to evaluate the effects of human PKD1 in relation to intestinal inflammation, using a co-culture model of intestinal epithelial Caco-2 cells and RAW264.7 macrophages. An inflammatory response was induced in the macrophages by lipopolysaccharide (LPS), upregulating the expression of tumour necrosis factor alpha (TNF-α), interleukin- (IL-) 1β, and IL-6 besides increasing the secretion of TNF-αprotein. The effect of administering PKD1 to Caco-2 was evaluated in relation to both amelioration of inflammation and the ability to suppress inflammation initiation. Administration of PKD1 (10–100 ng/ml) following induction of inflammation induced downregulation of TNF-αexpression in RAW264.7 cells. In addition, PKD1 administered for 3 h prior to LPS stimulation reduced the subsequent inflammatory response through downregulation of TNF-α, IL-1β, and IL-6 in RAW264.7 cells. These results demonstrate a potential role of PKD1 in the intercellular communication between intestinal epithelial and immune cells, proposing a protective effect of PKD1 on the induction of an inflammatory response in macrophages, an important aspect during the pathogenesis of IBD.

scholarly journals Gallic and butyric acids modulated NLRP3 inflammasome markers in a co-culture model of intestinal inflammation

2020 ◽  
Vol 146 ◽  
pp. 111835
Author(s):  
Ivan Luzardo-Ocampo ◽  
Guadalupe Loarca-Piña ◽  
Elvira Gonzalez de Mejia
Keyword(s):  
Nlrp3 Inflammasome ◽  
Intestinal Inflammation ◽  
Culture Model

Cinnamon subcritical water extract attenuates intestinal inflammation and enhances intestinal tight junction in a Caco-2 and RAW264.7 co-culture model

2019 ◽  
Vol 10 (7) ◽  
pp. 4350-4360 ◽  
Author(s):  
Min Seo Kim ◽  
Ji Yeon Kim
Keyword(s):  
Tight Junction ◽  
Tight Junctions ◽  
Intestinal Inflammation ◽  
Subcritical Water ◽  
Water Extract ◽  
Physiological Effects ◽  
Inflammation Model ◽  
Culture Model ◽  
Anti Inflammation

Cinnamon is known to have several physiological effects; the effects of Cinnamomum japonicum Sieb. on anti-inflammation and tight junctions were investigated using the cellular intestinal inflammation model.

Establishment of an In Vitro Co-Culture Model of the Piglet Gut to Study Inflammatory Response and Barrier Integrity

Planta Medica ◽  
2021 ◽  
Author(s):  
Theresa Schott ◽  
Nicole Reisinger ◽  
Klaus Teichmann ◽  
Jürgen König ◽  
Andrea Ladinig ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Mononuclear Cells ◽  
Intestinal Inflammation ◽  
Epithelial Barrier ◽  
Peripheral Blood Mononuclear ◽  
Barrier Integrity ◽  
Culture Model

AbstractIn intensive farming, piglets are exposed to various challenges that activate intestinal inflammatory processes, negatively affecting animal health and leading to economic losses. To study the role of the inflammatory response on epithelial barrier integrity, co-culture systems that mimic in vivo complexity are more and more preferred over cell monocultures. In this study, an in vitro gut co-culture model consisting of intestinal porcine epithelial cells and porcine peripheral blood mononuclear cells was established. The model provides an appropriate tool to study the role of the inflammatory response on epithelial barrier integrity and to screen for feed and food components, exerting beneficial effects on gut health. In the established model, inflammation-like reactions and damage of the epithelial barrier, indicated by a decrease of transepithelial electrical resistance, were elicited by activation of peripheral blood mononuclear cells via one of 3 stimuli: lipopolysaccharide, lipoteichoic acid, or concanavalin A. Two phytogenic substances that are commonly used as feed additives, licorice extract and oregano oil, have been shown to counteract the drop in transepithelial electrical resistance values in the gut co-culture model. The established co-culture model provides a powerful in vitro tool to study the role of intestinal inflammation on epithelial barrier integrity. As it consists of porcine epithelial and porcine blood cells it perfectly mimics in vivo conditions and imitates the inter-organ communication of the piglet gut. The developed model is useful to screen for nutritional components or drugs, having the potential to balance intestinal inflammation and strengthen the epithelial barrier integrity in piglets.

Indoleamine 2,3 dioxygenase downregulates intestinal inflammation

2001 ◽  
Vol 120 (5) ◽  
pp. A182-A182
Author(s):  
G GREGORY ◽  
W STENSON ◽  
R NEWBERRY
Keyword(s):  
Intestinal Inflammation ◽  
Indoleamine 2,3 Dioxygenase

Toll like receptors 2 and 4 are upregulated during intestinal inflammation

2001 ◽  
Vol 120 (5) ◽  
pp. A182-A183
Author(s):  
M HAUSMANN ◽  
S MESTERMANN ◽  
T SPOETTI ◽  
J SCHOELMERICH ◽  
T ANDUS ◽  
...  
Keyword(s):  
Intestinal Inflammation ◽  
Toll Like Receptors

Role of CD5 expression on TCRγδ T cell-differentiation and development of chronic intestinal inflammation

2001 ◽  
Vol 120 (5) ◽  
pp. A517-A517
Author(s):  
A MIZOGUCHI ◽  
E MIZOGUCHI ◽  
Y DEJONG ◽  
H TAKEDATSU ◽  
F PREFFER ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Intestinal Inflammation ◽  
T Cell Differentiation ◽  
Chronic Intestinal Inflammation

1120: Citrate and Vitamin E Blunt the SWL-Induced Free Radical Surge in an In-Vitro MDCK Cell Culture Model

2004 ◽  
Vol 171 (4S) ◽  
pp. 295-295
Author(s):  
Fernando C. Delvecchio ◽  
Ricardo M. Brizuela ◽  
Karen J. Byer ◽  
W. Patrick Springhart ◽  
Saeed R. Khan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Free Radical ◽  
Vitamin E ◽  
Mdck Cell ◽  
Cell Culture Model ◽  
Culture Model
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