Ethanolic extract of Betel ( Piper betle L.) and Chaphlu ( Piper sarmentosum Roxb.) dechlorophyllized using sedimentation process: Production, characteristics, and antioxidant activities

2020 ◽  
Vol 44 (12) ◽  
Author(s):  
Mohamed Tagrida ◽  
Soottawat Benjakul
Keyword(s):  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Piper Betle ◽  
Sedimentation Process ◽  
Production Characteristics

Determination of Antioxidant Activities and Bioactive Components of Foeniculum vulgare Mill. (Samonsabar)

2019 ◽  
Vol 30 (3) ◽  
pp. 16-22
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Scientific Information ◽  
Ethanolic Extract ◽  
The Body ◽  
World Health ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Bioactive Components ◽  
Foeniculum Vulgare

World Health Organization (WHO) estimated that 80% of the population of developing countries use traditional medicines, mostly natural plant products, for their primary health care needs. In the past few decades, the medicinal value of plants has been assumed more important dimension owing largely to the discovery that extracts from plants contain not only primary metabolites but also a diverse array of secondary metabolites with antioxidant potential. Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Antioxidants are vital substances which possess ability to protect the body from damage due to free radical-induced oxidative stress. The purpose of current study was to determine the antioxidant activities and bioactive components of Foeniculum vulgare (fennel) (Samonsabar) seeds by using UV Visible Spectrophotometer (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Aqueous extract of fennel seeds showed more antioxidant activity (IC50: 0.28 ug/ml) than ethanolic extract (IC50: 0.83 ug/ml) and comparable to standard antioxidant, ascorbic acid (IC50: 0.59 ug/ml). GC-MS analysis was fruitful in identification of compounds based on peak area, retention time, molecular formula, molecular weight, MS Fragmentions and pharmacological actions. Ten bioactive phytochemical compounds from aqueous extracts and 11 from ethanolic extract of fennel seeds were identified. These findings indicated that fennel seeds are potential to provide preventive properties against oxidative damage. These results will give scientific information for quality control of indigenous drug to herbal medicine users and local practitioners using fennel for different types of ailments

Management of Dermatophytoses by Foliose Lichen; Heterodermia diademata (Taylor) D.D. Awasthi

2017 ◽  
Vol 5 (2) ◽  
pp. 79-84
Author(s):  
Anand Pandey ◽  
◽  
Awadhesh Kumar ◽  
Anupam Dikshit ◽  
◽  
...  
Keyword(s):  
Fungicidal Activity ◽  
Trichophyton Rubrum ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Microsporum Gypseum ◽  
Superficial Infection ◽  
Recent Advancement ◽  
Secondary Substances ◽  
Foliose Lichen

Lichens are demarcated as an association of two distinct and dissimilar components; the mycobiont (a fungus) and the phycobionts (an alga) forming a composite organism. The recent advancement in science has paid much attention on the biological roles of lichen secondary substances; which are attributed with a lot of biological activities, such as antibacterial, antifungal, antiviral, anti-inflammatory, anti-tumor, and antioxidant activities. The current research aims to unravel the fungicidal activity of lichen Heterodermia diademata, ethanolic extract (HDE) against prevalent superficial infection causing dermatophytes viz., Trichophyton rubrum, T. mentagrophytes, Microsporum gypseum and M. canis. The HDE was found active against M. gypseum and T. mentagrophytes with MIC; 1.68 and 1.98 mg/ml and mg/ml respectively. The dermatophytes were also analyzed by molecular phylogeny of chs-1 gene for variability in MIC and prediction of susceptible pathogens.

scholarly journals Betel (Piper betle L.) leaf ethanolic extracts dechlorophyllized using different methods: antioxidant and antibacterial activities, and application for shelf-life extension of Nile tilapia (Oreochromis niloticus) fillets

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17630-17641
Author(s):  
Mohamed Tagrida ◽  
Soottawat Benjakul
Keyword(s):  
Shelf Life ◽  
Nile Tilapia ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Life Extension ◽  
Piper Betle ◽  
Refrigerated Storage ◽  
Betel Leaf ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Ethanolic Extracts

Betel leaf ethanolic extract dechlorophyllized using sedimentation (BLEE-SED) exhibited enhanced antioxidant and antibacterial activities. BLEE-SED could extend the shelf-life of Nile tilapia fillets during refrigerated storage (4 °C) up to 9 days.

Antitumor and antioxidant activities of purple potato ethanolic extract and its interaction with liposomes, albumin and plasmid DNA

2021 ◽  
Author(s):  
Paulina Anita Strugała ◽  
Anna Urbaniak ◽  
Patryk Kuryś ◽  
Aleksandra Włoch ◽  
Teresa Kral ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Antioxidative Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract

The aim of study was to broadly determine the biological activities of purple potato ethanolic extract of the Blue Congo variety (BCE). The antioxidative activity of BCE was determined in...

scholarly journals Phytochemical Analysis, Antioxidant and Anti-Inflammatory Activity of Calyces from Physalis peruviana

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Reina M. Toro ◽  
Diana M. Aragón ◽  
Luis F. Ospina ◽  
Freddy A. Ramos ◽  
Leonardo Castellanos
Keyword(s):  
Antioxidant Activities ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Physalis Peruviana ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Bio Assay

Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities. Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

scholarly journals ANTIDIABETIC AND ANTIOXIDANT ACTIVITIES OF ETHANOLIC EXTRACT OF PIPER BETLE L. LEAVES IN CATFISH, CLARIAS GARIEPINUS

2018 ◽  
Vol 11 (3) ◽  
pp. 194 ◽  
Author(s):  
Kavitha S ◽  
Parthasarathi Perumal
Keyword(s):  
Antioxidant Activities ◽  
Clarias Gariepinus ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Metformin Hydrochloride ◽  
Piper Betle ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Antioxidant Agents ◽  
Ic50 Values

 Objective: The present study was undertaken to assess the α-amylase inhibitory activity and antidiabetic experimental catfish model and antioxidant properties of Piper betle L. ethanolic (PBE) extract.Methods: The phytochemical analysis of PBE extract was performed. The PBE extract was tested for their inhibitory effect on the α-amylase assay, which compared to the control, acarbose. The absorbance was read at 540 nm using a spectrophotometer, and IC50 values were calculated. In this present investigation, diabetes mellitus was induced in catfish, Clarias gariepinus by epaxial musculature injection to glucose and standard drug, Metformin hydrochloride. After 24-h incubation, the treated fishes were dissected, and the blood, liver, tissue samples, and epaxial musculature regions were collected. In addition, the antioxidant properties of PBE were determined by 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical scavenging and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging (ABTS) assays.Results: The phytochemical screening of PBE revealed the presence of alkaloid, flavonoids, tannins, phenol, glycosides, sterols, saponins, and quinines. Furthermore, the values of (μg/ml) 3.038 and 7.672 α-amylase enzyme inhibition were excellent activity when compared to the acarbose. Moreover, elevated the glucose level (mg/dl) was estimated in blood 1.9±0.35, liver 0.5±0.25, tissue 0.2±0.25, and epaxial musculature 0.8±0.2 after 24-h incubation. The antioxidant effect of maximum activity was found in PBE; IC50 values (μg/ml) of DPPH and ABTS were 9.362 and 6.606, respectively.Conclusions: These studies might be responsible for the P. betle L. that was used as the new source of antidiabetic and antioxidant agents. 

scholarly journals Pharmacological Potential and Chemical Composition of Crocus sativus Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 10
Author(s):  
Olha Mykhailenko ◽  
Vilma Petrikaite ◽  
Michal Korinek ◽  
Fang-Rong Chang ◽  
Mohamed El-Shazly ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Crocus Sativus ◽  
Leaf Extracts ◽  
Hplc Fingerprint ◽  
Health Related ◽  
Pharmacological Potential

Crocus sativus L. (saffron) has been traditionally used as a food coloring or flavoring agent, but recent research has shown its potent pharmacological activity to tackle several health-related conditions. Crocus sp. leaves, and petals are the by-products of saffron production and are not usually used in the medicine or food industries. The present study was designed to determine the chemical composition of the water and ethanolic extracts of C. sativus leaves and test their cytotoxic activity against melanoma (IGR39) and triple-negative breast cancer (MDA-MB-231) cell lines by MTT assay. We also determined their anti-allergic, anti-inflammatory, and anti-viral activities. HPLC fingerprint analysis showed the presence of 16 compounds, including hydroxycinnamic acids, xanthones, flavonoids, and isoflavonoids, which could contribute to the extracts’ biological activities. For the first time, compounds such as tectoridin, iristectorigenin B, nigricin, and irigenin were identified in Crocus leaf extracts. The results showed that mangiferin (up to 2 mg/g dry weight) and isoorientin (8.5 mg/g dry weight) were the major active ingredients in the leaf extracts. The ethanolic extract reduced the viability of IGR39 and MDA-MB-231 cancer cells with EC50 = 410 ± 100 and 330 ± 40 µg/mL, respectively. It was more active than the aqueous extract. Kaempferol and quercetin were identified as the most active compounds. Our results showed that Crocus leaves contain secondary metabolites with potent cytotoxic and antioxidant activities.

scholarly journals Purification and Characterization of Bioactive Compounds Extracted from Suaeda Maritima Leaf and its Impact on Pathogenicity of Pseudomonas Aeruginosa in Catla Catla Fingerlings

2021 ◽  
Author(s):  
Beulah G ◽  
Divya D ◽  
Sampath Kumar N.S. ◽  
Sravya M.V.N. ◽  
Govinda Rao K ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
In Vivo Studies ◽  
Catla Catla ◽  
Active Fraction ◽  
Efficient Treatment ◽  
Suaeda Maritima

Abstract Incidence of various dreadful microbial infections and the development of antibiotic resistance by infection causative microbes are the main reasons for reducing aquaculture productivity. Hence, there is an immense need for the discovery of alternative and efficient treatment for quick recovery of diseased fishes. In the present study, Suaeda maritima leaf extracts (hexane, diethyl ether, ethanol, and water) were screened for in vitro and in vivo antibacterial and antioxidant activities. Out of all the four extracts, ethanolic extract showed highest antibacterial activity against S.aureus (4.9±1.3 mm), B.subtilis (1.6±0.3 mm), K.pneumonia (4.2±1.8 mm), and P.aeruginosa (4.1±1.2 mm). Similarly, antioxidant activity was also higher for ethanolic extract (500 µg/ml) based on DPPH radical scavenging ability (71.6±1.4 %) and reducing potential (149 μg/mL) assays. Further, ethanolic extract was purified consecutively via column chromatography and preparative TLC where an active fraction was selected based on highest antibacterial (10.1±1.4 mm) and antioxidant properties (82.3±2.8 %). Active fraction was loaded onto mass spectroscopy and identified the presence of four active constituents such as 1,2,9,10-tetramethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-3-yl) methanol; 3',7-Dimethoxy-3-hydroxyflavone; Saponin and (19R)9acetyl19hydroxy10,14dimethyl20oxopentacyclo[11.8.0.0<2,10>.0<4,9>.0<14,19>]henicos-17-yl-acetate. Besides, in vivo studies were conducted on Catla catla fingerlings infected with P. aeruginosa under laboratory conditions. The fingerlings were segregated into 5 groups, among which group 4 and 5 were treated with crude and purified extracts. Both the extracts were efficient in treating infected fingerlings and recorded 100% survival rate which is even better than group-3 treated with a synthetic antibiotic (77%). Hence, S. maritima leaf extract can be considered as a possible alternative medicine in aquaculture

scholarly journals Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

2020 ◽  
pp. 1-5
Author(s):  
M. Suleman Stephen ◽  
E. A. Adelakun ◽  
J. H. Kanus ◽  
Meshack M. Gideon
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Acetone Extract ◽  
Apium Graveolens ◽  
Isolation And Identification ◽  
Celery Plant

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

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