DNA damage and genetic aberration induced via different sized silver nanoparticles: Therapeutic approaches of Casimiroa edulis and Glycosmis pentaphylla leaves extracts

2020 ◽  
Vol 44 (10) ◽  
Author(s):  
Sanaa A. Ali ◽  
Azza F. Arafa ◽  
Hanan F. Aly ◽  
Nabaweya A. Ibrahim ◽  
Mai O. Kadry ◽  
...  
Keyword(s):  
Dna Damage ◽  
Silver Nanoparticles ◽  
Therapeutic Approaches ◽  
Casimiroa Edulis ◽  
Genetic Aberration

Curcumin-containing Silver Nanoparticles prevent carbon tetrachlorideinduced hepatotoxicity in mice

2020 ◽  
Vol 23 ◽  
Author(s):  
Hossam Ebaid ◽  
Mohamed Habila ◽  
Iftekhar Hassan ◽  
Jameel Al-Tamimi ◽  
Mohamed S. Omar ◽  
...  
Keyword(s):  
Dna Damage ◽  
Silver Nanoparticles ◽  
Nuclear Dna ◽  
Liquid Paraffin ◽  
Tail Length ◽  
Hepatic Tissue ◽  
Tissue Destruction ◽  
Group 2 ◽  
The Impact

Background: Hepatotoxicity remains an important clinical challenge. Hepatotoxicity observed in response to toxins and hazardous chemicals may be alleviated by delivery of the curcumin in silver nanoparticles (AgNPs-curcumin). In this study, we examined the impact of AgNPs-curcumin in a mouse model of carbon tetrachloride (CCl4)-induced hepatic injury. Methods: Male C57BL/6 mice were divided into three groups (n=8 per group). Mice in group 1 were treated with vehicle control alone, while mice in Group 2 received a single intraperitoneal injection of 1 ml/kg CCl4 in liquid paraffin (1:1 v/v). Mice in group 3 were treated with 2.5 mg/kg AgNPs-curcumin twice per week for three weeks after the CCl4 challenge. Results: Administration of CCL4 resulted in oxidative dysregulation, including significant reductions in reduced glutathione and concomitant elevations in the level of malondialdehyde (MDA). CCL4 challenge also resulted in elevated levels of serum aspartate transaminase (AST) and alanine transaminase (ALT); these findings were associated with the destruction of hepatic tissues. Treatment with AgNPs-curcumin prevented oxidative imbalance, hepatic dysfunction, and tissue destruction. A comet assay revealed that CCl4 challenge resulted in significant DNA damage as documented by a 70% increase in nuclear DNA tail-length; treatment with AgNPs-curcumin inhibited the CCL4-mediated increase in nuclear DNA tail-length by 34%. Conclusion: Administration of AgNPs-curcumin resulted in significant antioxidant activity in vivo. This agent has the potential to prevent the hepatic tissue destruction and DNA damage that results from direct exposure to CCL4.

Silver Nanoparticles and Carbon Nanotubes Induced DNA Damage in Mice Evaluated by Single Cell Gel Electrophoresis

2015 ◽  
Vol 357 (1) ◽  
pp. 210-217 ◽  
Author(s):  
Kumud Kant Awasthi ◽  
Anjali Awasthi ◽  
Rajbala Verma ◽  
Inderpal Soni ◽  
Kamlendra Awasthi ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Single Cell Gel Electrophoresis

scholarly journals Size-Dependent Effect of Silver Nanoparticles on the Tumor Necrosis Factor α-Induced DNA Damage Response

2019 ◽  
Vol 20 (5) ◽  
pp. 1038 ◽  
Author(s):  
Alaa Fehaid ◽  
Akiyoshi Taniguchi
Keyword(s):  
Dna Damage ◽  
Silver Nanoparticles ◽  
Tumor Necrosis Factor ◽  
Cell Surface ◽  
Dna Damage Response ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Damage Response ◽  
Factor Α ◽  
Necrosis Factor

Silver nanoparticles (AgNPs) are widely used in many consumer products due to their anti-inflammatory properties. Therefore, the effect of exposure to AgNPs should be investigated in diseased states in addition to healthy ones. Tumor necrosis factor-α (TNFα) is a major cytokine that is highly expressed in many diseased conditions, such as inflammatory diseases, sepsis, and cancer. We investigated the effects of two different sizes of AgNPs on the TNFα-induced DNA damage response. Cells were exposed to 10 and 200 nm AgNPs separately and the results showed that the 200 nm AgNPs had a lower cytotoxic effect with a higher percent of cellular uptake compared to the 10 nm AgNPs. Moreover, analysis of reactive oxygen species (ROS) generation and DNA damage indicated that TNFα-induced ROS-mediated DNA damage was reduced by 200 nm AgNPs, but not by 10 nm AgNPs. Tumor necrosis factor receptor 1 (TNFR1) was localized on the cell surface after TNFα exposure with or without 10 nm AgNPs. In contrast, the expression of TNFR1 on the cell surface was reduced by the 200 nm AgNPs. These results suggested that exposure of cells to 200 nm AgNPs reduces the TNFα-induced DNA damage response via reducing the surface expression of TNFR1, thus reducing the signal transduction of TNFα.

Effect of bixin on DNA damage and cell death induced by doxorubicin in HL60 cell line

2016 ◽  
Vol 35 (12) ◽  
pp. 1319-1327 ◽  
Author(s):  
GC Santos ◽  
MR Almeida ◽  
LMG Antunes ◽  
MLP Bianchi
Keyword(s):  
Dna Damage ◽  
Hl60 Cell ◽  
Red Pigment ◽  
Combined Treatments ◽  
Therapeutic Approaches ◽  
Cytotoxic Effects ◽  
Hl60 Cells ◽  
Apoptotic Effect ◽  
Induction Of Apoptosis ◽  
Hl60 Cell Line

Bixin is a natural red pigment extracted from annatto. Although it is widely used as a coloring agent in food, there are few studies about the effect of this carotenoid on DNA. This study aimed to investigate the effects of bixin on cytotoxicity and genotoxicity induced by doxorubicin in HL60 cells. At concentrations above 0.3 μg/mL, bixin demonstrated cytotoxic effects in HL60 cells. Furthermore, this carotenoid was neither mutagenic nor genotoxic to HL60 cells and reduced the DNA damage induced by doxorubicin. Bixin and doxorubicin showed no apoptotic effect in HL60 cells, but the simultaneous combined treatments showed an increase in the percentage of apoptotic cells. In conclusion, our results showed that bixin modulates the cytotoxicity of doxorubicin via induction of apoptosis. The results of this study provide more knowledge about the toxic effects of anticancer treatments and how the natural compounds can be useful on these therapeutic approaches.

Preparation of silver nanoparticles as antibacterial agents through DNA damage

2019 ◽  
Vol 34 (14) ◽  
pp. 867-879 ◽  
Author(s):  
Walaa Saeed Abbas ◽  
Zeenah Waheed Atwan ◽  
Zainab R. Abdulhussein ◽  
M.A. Mahdi
Keyword(s):  
Dna Damage ◽  
Silver Nanoparticles ◽  
Antibacterial Agents

Oxidative DNA damage corresponds to the long term survival of human cells treated with silver nanoparticles

2013 ◽  
Vol 219 (2) ◽  
pp. 151-159 ◽  
Author(s):  
Marcin Kruszewski ◽  
Iwona Grądzka ◽  
Teresa Bartłomiejczyk ◽  
Jadwiga Chwastowska ◽  
Sylwester Sommer ◽  
...  
Keyword(s):  
Dna Damage ◽  
Silver Nanoparticles ◽  
Oxidative Dna Damage ◽  
Human Cells ◽  
Term Survival ◽  
Long Term Survival

scholarly journals Cationic Antimicrobial Peptides and Biogenic Silver Nanoparticles Kill Mycobacteria without Eliciting DNA Damage and Cytotoxicity in Mouse Macrophages

2013 ◽  
Vol 57 (8) ◽  
pp. 3688-3698 ◽  
Author(s):  
Soumitra Mohanty ◽  
Prajna Jena ◽  
Ranjit Mehta ◽  
Rashmirekha Pati ◽  
Birendranath Banerjee ◽  
...  
Keyword(s):  
Dna Damage ◽  
Silver Nanoparticles ◽  
Antimicrobial Peptides ◽  
Fluorescein Isothiocyanate ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Content Type ◽  
Biogenic Silver Nanoparticles ◽  
Transmission Electron ◽  
Mouse Macrophages

ABSTRACTWith the emergence of multidrug-resistant mycobacterial strains, better therapeutic strategies are required for the successful treatment of the infection. Although antimicrobial peptides (AMPs) and silver nanoparticles (AgNPs) are becoming one of the popular antibacterial agents, their antimycobacterial potential is not fully evaluated. In this study, we synthesized biogenic-silver nanoparticles using bacterial, fungal, and plant biomasses and analyzed their antibacterial activities in combination with AMPs against mycobacteria.Mycobacterium smegmatiswas found to be more susceptible to AgNPs compared toM. marinum. We found that NK-2 showed enhanced killing effect with NP-1 and NP-2 biogenic nanoparticles at a 0.5-ppm concentration, whereas LLKKK-18 showed antibacterial activity only with NP-2 at 0.5-ppm dose againstM. smegmatis. In case ofM. marinumNK-2 did not show any additive activity with NP-1 and NP-2 and LLKKK-18 alone completely inhibited the bacterial growth. Both NP-1 and NP-2 also showed increased killing ofM. smegmatisin combination with the antituberculosis drug rifampin. The sizes and shapes of the AgNPs were determined by transmission electron microscopy and dynamic light scattering. AgNPs showed no cytotoxic or DNA damage effects on macrophages at the mycobactericidal dose, whereas treatment with higher doses of AgNPs caused toxicity and micronuclei formation in cytokinesis blocked cells. Macrophages actively endocytosed fluorescein isothiocyanate-labeled AgNPs resulting in nitric oxide independent intracellular killing ofM. smegmatis. Apoptosis and cell cycle studies showed that treatment with higher dose of AgNPs arrested macrophages at the G1-phase. In summary, our data suggest the combined effect of biogenic-AgNPs and antimicrobial peptides as a promising antimycobacterial template.

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