Three common caffeoylquinic acids as potential hypoglycemic nutraceuticals: Evaluation of α‐glucosidase inhibitory activity and glucose consumption in HepG2 cells

2020 ◽  
Vol 44 (9) ◽  
Author(s):  
Yongsheng Chen ◽  
Sheng Geng ◽  
Benguo Liu
Keyword(s):  
Inhibitory Activity ◽  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Caffeoylquinic Acids

Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

2018 ◽  
Author(s):  
Anna Adams ◽  
Radha Krishna Murthy Bulusu ◽  
Nikita Mukhitov ◽  
Jose Mendoza-Cortes ◽  
Michael Roper
Keyword(s):  
Real Time ◽  
Glucose Concentration ◽  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Microfluidic System ◽  
Structure And Function ◽  
Online Measurement ◽  
Fluorescent Assay ◽  
And Function ◽  
Integrated Bioreactor

In this work, we developed a microfluidic bioreactor for optimizing growth and maintaining structure and function of HepG2, and when desired, the device could be removed and the extracellular output from the bioreactor combined with enzymatic glucose reagents into a droplet-based microfluidic system. The intensity of the resulting fluorescent assay product in the droplets was measured, and was directly correlated to glucose concentration, allowing the effect of insulin on glucose consumption in the HepG2 cells to be observed and quantified online and in near real-time.

Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

2018 ◽  
Author(s):  
Anna Adams ◽  
Radha Krishna Murthy Bulusu ◽  
Nikita Mukhitov ◽  
Jose Mendoza-Cortes ◽  
Michael Roper
Keyword(s):  
Real Time ◽  
Glucose Concentration ◽  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Microfluidic System ◽  
Structure And Function ◽  
Online Measurement ◽  
Fluorescent Assay ◽  
And Function ◽  
Integrated Bioreactor

In this work, we developed a microfluidic bioreactor for optimizing growth and maintaining structure and function of HepG2, and when desired, the device could be removed and the extracellular output from the bioreactor combined with enzymatic glucose reagents into a droplet-based microfluidic system. The intensity of the resulting fluorescent assay product in the droplets was measured, and was directly correlated to glucose concentration, allowing the effect of insulin on glucose consumption in the HepG2 cells to be observed and quantified online and in near real-time.

scholarly journals Antimicrobial and Efflux Pump Inhibitory Activity of Caffeoylquinic Acids from Artemisia absinthium against Gram-Positive Pathogenic Bacteria

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18127 ◽  
Author(s):  
Yiannis C. Fiamegos ◽  
Panagiotis L. Kastritis ◽  
Vassiliki Exarchou ◽  
Haley Han ◽  
Alexandre M. J. J. Bonvin ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Efflux Pump ◽  
Artemisia Absinthium ◽  
Gram Positive ◽  
Caffeoylquinic Acids

scholarly journals Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-8
Author(s):  
Xiao-Tian Zhang ◽  
Chun-Jiang Yu ◽  
Jian-Wei Liu ◽  
Yan-Ping Zhang ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Palmitic Acid ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Glucose Consumption ◽  
Control Group ◽  
High Dose ◽  
Oxygen Species ◽  
Insulin Resistant

We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P< 0.001); however, QJJ restored HepG2 cell survival (P< 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (allP< 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (allP< 0.05). Furthermore, high-dose QJJ reduced both TNF-αand IL-6 levels as compared to the IR group (allP< 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.

scholarly journals Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

2019 ◽  
Vol 91 (8) ◽  
pp. 5184-5190 ◽  
Author(s):  
Anna G. Adams ◽  
Radha Krishna Murthy Bulusu ◽  
Nikita Mukhitov ◽  
Jose L. Mendoza-Cortes ◽  
Michael G. Roper
Keyword(s):  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Enzymatic Assay ◽  
Online Measurement ◽  
Integrated Bioreactor

Purification of naringin and neohesperidin from Huyou (Citrus changshanensis) fruit and their effects on glucose consumption in human HepG2 cells

2012 ◽  
Vol 135 (3) ◽  
pp. 1471-1478 ◽  
Author(s):  
Jiukai Zhang ◽  
Chongde Sun ◽  
Youyou Yan ◽  
Qingjun Chen ◽  
Fenglei Luo ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Glucose Consumption

scholarly journals PCB 126 perturbs hypoxia-induced HIF-1α activity and glucose consumption in human HepG2 cells

2014 ◽  
Vol 66 (8) ◽  
pp. 377-382 ◽  
Author(s):  
Sabine U. Vorrink ◽  
Ehab H. Sarsour ◽  
Alicia K. Olivier ◽  
Larry W. Robertson ◽  
Prabhat C. Goswami ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Pcb 126

ChemInform Abstract: Benzyl Glycosides from the Aerial Parts of Gynostemma laxum and Their NF-ϰB Inhibitory Activity in HepG2 Cells

ChemInform ◽  
2012 ◽  
Vol 43 (9) ◽  
pp. no-no
Author(s):  
Pham Thanh Ky ◽  
Pham Tuan Anh ◽  
Phan Van Kiem ◽  
Chau Van Minh ◽  
Nguyen Xuan Nhiem ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Hepg2 Cells ◽  
Aerial Parts

scholarly journals Effervescent Granules Prepared UsingEucommia ulmoidesOliv. and Moso Bamboo Leaves: Hypoglycemic Activity in HepG2 Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-6
Author(s):  
Xiang-Zhou Li ◽  
Sheng Zhang
Keyword(s):  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Synergistic Action ◽  
Moso Bamboo ◽  
Hypoglycemic Activity ◽  
Hypoglycemic Effect ◽  
Western China ◽  
Phyllostachys Pubescens ◽  
Chlorogenic Acids ◽  
Bamboo Leaves

Eucommia ulmoidesOliv. (E. ulmoidesOliv.) and moso bamboo (Phyllostachys pubescens) leaves are used as folk medicines in central-western China to treat diabetes. To investigate the hypoglycemic activity of the effervescent granules prepared usingE. ulmoidesOliv.and moso bamboo leaves (EBEG) in HepG2 cells, EBEG were prepared with 5% of each of polysaccharides and chlorogenic acids from moso bamboo andE. ulmoidesOliv.leaves, respectively. HepG2 cells cultured in a high-glucose medium were classified into different groups. The results displayed EBEG-treated cells showed better glucose utilization than the negative controls; thus, the hypoglycemic effect of EBEG was much greater than that of granules prepared using either component alone, thereby indicating that this effect was due to a synergistic action of the components. Further, glucose consumption levels in the cells treated with EBEG (156.35% at 200 μg/mL) and the positive controls (metformin, 162.29%; insulin, 161.52%) were similar. Thus, EBEG exhibited good potential for use as a natural antidiabetic agent. The hypoglycemic effect of EBEG could be due to the synergistic action of polysaccharides from the moso bamboo leaves and chlorogenic acids fromE. ulmoidesOliv. leaves via the inhibition of alpha-glucosidase and glucose-6-phosphate displacement enzyme.

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