The synergistic effect of cell wall extracted from probiotic biomass containing Lactobacillus acidophilus CL1285, L. casei LBC80R, and L. rhamnosus CLR2 on the anticancer activity of cranberry juice—HPLC fractions

2020 ◽  
Vol 44 (5) ◽  
Author(s):  
Kerlynn Desrouillères ◽  
Mathieu Millette ◽  
Leila Bagheri ◽  
Behnoush Maherani ◽  
Majid Jamshidian ◽  
...  
Keyword(s):  
Cell Wall ◽  
Synergistic Effect ◽  
Anticancer Activity ◽  
Lactobacillus Acidophilus ◽  
Cranberry Juice ◽  
Probiotic Biomass

Mitogenic Activity of Cell Wall Components from Lactobacillus acidophilus

1993 ◽  
Vol 64 (5) ◽  
pp. 505-511 ◽  
Author(s):  
Masahiro YAMADA ◽  
Haruki KITAZAWA ◽  
Junko UEMURA ◽  
Tadao SAITOH ◽  
Takatoshi ITOH
Keyword(s):  
Cell Wall ◽  
Lactobacillus Acidophilus ◽  
Mitogenic Activity ◽  
Cell Wall Components ◽  
Wall Components

scholarly journals Evaluation of a Synergistic Effect of L-Arginine on the Anticancer Activity of Doxorubicin by Using a Co-culture System

2020 ◽  
Vol 36 (10) ◽  
pp. 1279-1283
Author(s):  
Haitao FENG ◽  
Akihiro KISHIMURA ◽  
Takeshi MORI ◽  
Yoshiki KATAYAMA
Keyword(s):  
Synergistic Effect ◽  
Anticancer Activity ◽  
Culture System

Synthesis and anticancer activity of paclitaxel−coumarin conjugate

2021 ◽  
Vol 18 ◽  
Author(s):  
Zurong Song ◽  
Qin Lu ◽  
Ali Tao ◽  
Tianchen Wu
Keyword(s):  
Flow Cytometry ◽  
Synergistic Effect ◽  
Anticancer Activity ◽  
Mtt Assay ◽  
Scratch Test ◽  
The Novel ◽  
Effective Strategy ◽  
Test Results ◽  
Antibacterial Effects ◽  
Anti Tumor Activity

Background: Paclitaxel, a natural diterpenoid compound, has anti-tumor effect by acting on tubulin; coumarin, another kind of natural product, has anti-tumor, antibacterial effects and so on. Moreover coumarin has fluorescence. Objective: Multi target to combat tumor is an effective strategy in drug design. Therefore, combination of paclitaxel with other acticive moleculer to explore the novel lead with multi-functons is in demand. Materials and Methods: To synthsize paclitaxel-coumarin conjugate via click chemistry and to investigate anticancer activity by MTT assay and the scratch test. Results and Discussion: The results of MTT assay showed that compared with paclitaxel, the anti-tumor activity of the conjugate was significantly improved. The results of flow cytometry showed that the conjugate had stronger ability to induce apoptosis. The scratch test results showed that the conjugate had better anti- metastasis ability than paclitaxel. Conclusion: These findings indicated that paclitaxel and coumarin had synergistic effect, which provided a new idea for the development of paclitaxel monitored by fluorescence.

scholarly journals Murein Hydrolase Activity in the Surface Layer of Lactobacillus acidophilus ATCC 4356

2008 ◽  
Vol 74 (24) ◽  
pp. 7824-7827 ◽  
Author(s):  
Mariano Prado Acosta ◽  
María Mercedes Palomino ◽  
Mariana C. Allievi ◽  
Carmen Sanchez Rivas ◽  
Sandra M. Ruzal
Keyword(s):  
Cell Wall ◽  
Surface Layer ◽  
Lactobacillus Acidophilus ◽  
Hydrolase Activity ◽  
Cloning And Expression ◽  
Functional Verification ◽  
Sequence Comparisons ◽  
C Terminus ◽  
Endopeptidase Activity ◽  
Murein Hydrolase

ABSTRACT We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydrolase activity was predicted to be located at the C terminus. Subsequent cloning and expression of the C-terminal domain in Bacillus subtilis resulted in the functional verification of the enzymatic activity.

Antibiotic susceptibility of different lactic acid bacteria strains

2011 ◽  
Vol 2 (4) ◽  
pp. 335-339 ◽  
Author(s):  
N. Karapetkov ◽  
R. Georgieva ◽  
N. Rumyan ◽  
E. Karaivanova
Keyword(s):  
Cell Wall ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Acidophilus ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Helveticus ◽  
Lactobacillus Delbrueckii ◽  
Cell Wall Synthesis ◽  
High Susceptibility ◽  
Lactobacillus Delbrueckii Subsp

Five lactic acid bacteria (LAB) strains belonging to species Lactobacillus acidophilus, Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis and Streptococcus thermophilus were tested for their susceptibility to 27 antibiotics. The minimum inhibitory concentrations of each antimicrobial were determined using a microdilution test. Among the strains a high susceptibility was detected for most of the cell-wall synthesis inhibitors (penicillins, cefoxitin and vancomycin) and resistance toward inhibitors of DNA synthesis (trimethoprim/sulfonamides and fluoroquinolones). Generally, the Lactobacillus strains were inhibited by antibiotics such as chloramphenicol, erythromycin and tetracycline at breakpoint levels lower or equal to the levels defined by the European Food Safety Authority. Despite the very similar profile of S. thermophilus LC201 to lactobacilli, the detection of resistance toward erythromycin necessitates the performance of additional tests in order to prove the absence of transferable resistance genes.

Study of Adhesion of Lactobacillus casei CRL 431 to Ileal Intestinal Cells of Mice

1999 ◽  
Vol 62 (12) ◽  
pp. 1430-1434 ◽  
Author(s):  
VILMA I. MORATA de AMBROSINI ◽  
SILVIA N. GONZALEZ ◽  
G. OLIVER
Keyword(s):  
Cell Wall ◽  
Immune System ◽  
Epithelial Cells ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Casei ◽  
Intestinal Cells ◽  
Surface Molecules ◽  
Good Ability ◽  
Initial Stage

It is well known that the cell wall of Lactobacillus casei CRL 431, a strain present in probiotics, presents lectinlike surface molecules. Presence of these molecules stimulates the immune system. Given the role that lectins and lectinlike substances play in the adhesion phenomenon, it is probable that this is an initial stage in the immunostimulation produced by this bacterium. To confirm this, adhesion of this microorganism to exfoliated mouse ileal epithelial cells was studied in vitro. Other L. casei strains isolated from adult human intestines and one of dairy origin were also examined for their ability to adhere to ileal epithelial cells. Another strain, which was included in the present study, was Lactobacillus acidophilus CRL 730. L. casei strains isolated from humans showed good ability to adhere to ileal epithelial cells, whereas L. casei isolated from dairy origin did not. Adhesion was only observed at 37°C and at a pH between 6 and 7.5. The exposure time needed for highest adhesion was 30 min. Presence of lectinlike substances on the surface of L. casei CRL 431 is important to this adhesion phenomenon, since adherence capacity was lost after removal of these substances.

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