Yunnan Baiyao diminishes lipopolysaccharide‐induced inflammation in osteoclasts

2020 ◽  
Vol 44 (6) ◽  
Author(s):  
Xiaobin Ren ◽  
Yanping Zhu ◽  
Liangkun Xie ◽  
Mingzhu Zhang ◽  
Lihui Gao ◽  
...  
Keyword(s):  
2018 ◽  
Vol 28 (3) ◽  
pp. 221-225 ◽  
Author(s):  
Colleen Tansey ◽  
Melissa L. Wiebe ◽  
Gabrielle C. Hybki ◽  
Jeffrey E. Patlogar ◽  
Lisa A. Murphy ◽  
...  

2017 ◽  
Vol 16 (02) ◽  
pp. 80-81 ◽  
Author(s):  
Jihe Zhu ◽  
Blagica Arsovska ◽  
Kristina Kozovska

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Xiyao Pang ◽  
Yanqiu Wang ◽  
Jintao Wu ◽  
Zhou Zhou ◽  
Tao Xu ◽  
...  

Yunnan Baiyao is a traditional Chinese herbal remedy that has long been used for its characteristics of wound healing, bone regeneration, and anti-inflammation. However, the effects of Yunnan Baiyao on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) and the potential mechanisms remain unclear. The aim of this study was to investigate the odonto/osteogenic differentiation effects of Yunnan Baiyao on SCAPs and the underlying mechanisms involved. SCAPs were isolated and cocultured with Yunnan Baiyao conditioned media. The proliferation ability was determined by cell counting kit 8 and flow cytometry. The differentiation capacity and the involvement of NF-κB pathway were investigated by alkaline phosphatase assay, alizarin red staining, immunofluorescence assay, real-time RT-PCR, and western blot analyses. Yunnan Baiyao conditioned medium at the concentration of 50 μg/mL upregulated alkaline phosphatase activity, induced more mineralized nodules, and increased the expression of odonto/osteogenic genes/proteins (e.g., OCN/OCN, OPN/OPN, OSX/OSX, RUNX2/RUNX2, ALP/ALP, COL-I/COL-I, DMP1, DSP/DSPP) of SCAPs. In addition, the expression of cytoplasmic phos-IκBα, phos-P65, and nuclear P65 was significantly increased in Yunnan Baiyao conditioned medium treated SCAPs in a time-dependent manner. Conversely, the differentiation of Yunnan Baiyao conditioned medium treated SCAPs was obviously inhibited when these stem cells were cocultured with the specific NF-κB inhibitor BMS345541. Yunnan Baiyao can promote the odonto/osteogenic differentiation of SCAPs via the NF-κB signaling pathway.


2019 ◽  
Vol 814 ◽  
pp. 365-371
Author(s):  
Si Tong Lu ◽  
Dong Ying Zhang ◽  
Zhang Hu ◽  
Si Dong Li ◽  
Pu Wang Li

In this paper, chitosan and caffeic acid were used as starting materials to prepare chitosan caffeates by reflux-heating and freeze-drying. The structures of chitosan caffeates were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and potentiometric titration. At the same time, the physical properties of chitosan caffeates were tested and the hemostatic properties were evaluated. The results showed that four chitosan caffeates with different mass ratios of chitosan and caffeic acid (1:1, 1:2, 1:4, 1:6) had been successfully prepared, which enhanced the water solubility. FTIR analysis demonstrated that caffeic acid had been successfully grafted onto chitosan chains. XRD showed that the crystal form of chitosan changed to some extent and the chain had some regularity in some directions, but its crystallinity reduced. Chitosan caffeates, particularly mass ratio of 1:1, showed excellent hemostatic properties and even better than chitosan and the positive control (Yunnan Baiyao), which were expected to be developed as an effective biomaterial for hemostasis.


2013 ◽  
Vol 51 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Zu-Guo Zhao ◽  
Shuang-Shuang Yan ◽  
Yun-Mei Yu ◽  
Na Mi ◽  
La-Xi Zhang ◽  
...  

2012 ◽  
Vol 20 (12) ◽  
pp. 3379-3383 ◽  
Author(s):  
E. J. Ladas ◽  
J. B. Karlik ◽  
D. Rooney ◽  
K. Taromina ◽  
D. H. Ndao ◽  
...  

2016 ◽  
Vol 1591 (1) ◽  
pp. 184-184
Keyword(s):  

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