Continuous preparation and characterization of immunomodulatory peptides from type II collagen by a novel immobilized enzyme membrane reactor with improved performance

2019 ◽  
Vol 43 (7) ◽  
Author(s):  
Hui Cao ◽  
Jifang Cao ◽  
Yujun Zhang ◽  
Tai Ye ◽  
Jin Song Yu ◽  
...  
Keyword(s):  
Membrane Reactor ◽  
Immobilized Enzyme ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Enzyme Membrane Reactor ◽  
Continuous Preparation ◽  
Improved Performance ◽  
Immunomodulatory Peptides

Characterization of human type II procollagen and collagen-specific antibodies and their application to the study of human type II collagen processing and ultrastructure

1997 ◽  
Vol 16 (1) ◽  
pp. 29-39 ◽  
Author(s):  
Sergio A. Jimenez ◽  
Leena Ala-Kokko ◽  
Darwin J. Prockop ◽  
Carmen F. Merryman ◽  
Nora Shepard ◽  
...  
Keyword(s):  
Type Ii Collagen ◽  
Type Ii ◽  
Specific Antibodies ◽  
Human Type

scholarly journals Phenotypic characterization of type II collagen-induced arthritis in Wistar rats

2015 ◽  
Vol 10 (4) ◽  
pp. 1483-1488 ◽  
Author(s):  
HOU-PAN SONG ◽  
XIN LI ◽  
RONG YU ◽  
GUANG ZENG ◽  
ZHEN-YI YUAN ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Type Ii Collagen ◽  
Phenotypic Characterization ◽  
Type Ii ◽  
Collagen Induced Arthritis

THE EFFECT OF SUBSTRATE MASS TRANSFER LIMITATIONS IN AN IMMOBILIZED ENZYME MEMBRANE REACTOR

1980 ◽  
Vol 7 (1-3) ◽  
pp. 145-157 ◽  
Author(s):  
GUIDO GRECO ◽  
FRANCESCO ALFANI ◽  
MARIA CANTARELLA ◽  
LILIANA GIANFREDA ◽  
ROSARIA PALESCANDOLO ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Membrane Reactor ◽  
Immobilized Enzyme ◽  
Enzyme Membrane Reactor

scholarly journals T Cell Immunity to Type II Collagen in the Biobreeding Rat: The Identification and Characterization of RT1u-Restricted T Cell Epitopes on α1(II)

2004 ◽  
Vol 173 (3) ◽  
pp. 1795-1801 ◽  
Author(s):  
Michael A. Cremer ◽  
Xiu J. Ye ◽  
Linda K. Myers ◽  
David D. Brand ◽  
Edward F. Rosloniec ◽  
...  
Keyword(s):  
T Cell ◽  
Type Ii Collagen ◽  
T Cell Immunity ◽  
Type Ii ◽  
T Cell Epitopes ◽  
Cell Immunity ◽  
Biobreeding Rat ◽  
Identification And Characterization

Characterization of a Microbial Transglutaminase Cross-linked Type II Collagen Scaffold

2006 ◽  
Vol 0 (0) ◽  
pp. 060706073730024
Author(s):  
Damien M. O Halloran ◽  
Russell J. Collighan ◽  
Martin Griffin ◽  
Abhay S. Pandit
Keyword(s):  
Collagen Scaffold ◽  
Type Ii Collagen ◽  
Microbial Transglutaminase ◽  
Type Ii

scholarly journals Development and Characterization of an Enzyme Membrane Reactor for Fructo-Oligosaccharide Production

Membranes ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 148 ◽  
Author(s):  
Burghardt ◽  
Coletta ◽  
van der Bolt ◽  
Ebrahimi ◽  
Gerlach ◽  
...  
Keyword(s):  
Membrane Reactor ◽  
Kluyveromyces Lactis ◽  
Calorific Value ◽  
Feed Additives ◽  
Efficient Production ◽  
Enzyme Membrane Reactor ◽  
Food And Feed ◽  
A Cell ◽  
Enzyme Molecules

Fructo-oligosaccharides (FOS) are linear fructans comprising 2–5 fructose units linked to a terminal glucose residue. They are widely used as food and feed additives due to their sweetness, low calorific value, and prebiotic properties. Here we describe the synthesis of FOS catalyzed by a cell-free crude enzyme solution containing recombinant fructosyltransferase (1-FFT) produced in the yeast Kluyveromyces lactis. During the enzyme catalysis, glucose accumulates as a by-product and eventually inhibits FOS production. We therefore used an enzyme membrane reactor (EMR) to achieve the continuous removal of glucose and the simultaneous replenishment of sucrose. We observed a loss of flux during the reaction with the characteristics of complete pore blocking, probably caused by a combination of proteins (enzyme molecules) and polysaccharides (FOS). Such complex fouling mechanisms must be overcome to achieve the efficient production of FOS using EMR systems.

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