Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells

2018 ◽  
pp. e12722
Author(s):  
Li Fang ◽  
Dayong Ren ◽  
Zuhao Wang ◽  
Chunlei Liu ◽  
Ji Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Umbilical Vein ◽  
Protective Role ◽  
Stress Injury ◽  
Human Umbilical Vein ◽  
Oxidative Stress Injury ◽  
Umbilical Vein Endothelial Cells

scholarly journals Puerarin Attenuates LPS-Induced Inflammatory Responses and Oxidative Stress Injury in Human Umbilical Vein Endothelial Cells through Mitochondrial Quality Control

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Xing Chang ◽  
Tian Zhang ◽  
Dong Liu ◽  
Qingyan Meng ◽  
Peizheng Yan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Quality Control ◽  
Endothelial Cells ◽  
Protective Effect ◽  
Umbilical Vein ◽  
Mitochondrial Quality Control ◽  
Stress Injury ◽  
Oxidative Stress Injury ◽  
Umbilical Vein Endothelial Cells ◽  
And Oxidative Stress

Atherosclerosis is closely associated with the inflammatory reaction of vascular endothelial cells. Puerarin (Pue), the main active component isolated from the rhizome of Pueraria lobata, is an isoflavone compound with potent antioxidant properties. Although Pue exhibits promising antiatherosclerotic pharmacological effects, only a few studies have reported its protective effect on endothelial cells. This study found that Pue could partly regulate mitochondrial function in human umbilical vein endothelial cells (HUVECs) and reduce or inhibit lipopolysaccharide-induced inflammatory reactions and oxidative stress injury in HUVECs, likely via mitochondrial quality control. Furthermore, the protective effect of Pue on HUVECs was closely related to the SIRT-1 signaling pathway. Pue increased autophagy and mitochondrial antioxidant potential via increased SIRT-1 expression, reducing excessive production of ROS and inhibiting the expression of inflammatory factors and oxidative stress injury. Therefore, Pue may improve mitochondrial respiratory function and energy metabolism, increasing the vulnerability of HUVECs to an inflammatory state.

scholarly journals Dietary Nucleotides Retard Oxidative Stress-Induced Senescence of Human Umbilical Vein Endothelial Cells

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3279
Author(s):  
Na Zhu ◽  
Xinran Liu ◽  
Meihong Xu ◽  
Yong Li
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Cell Viability ◽  
Mitochondrial Function ◽  
Umbilical Vein ◽  
Lower Percentage ◽  
Human Umbilical Vein ◽  
Dietary Nucleotides ◽  
Umbilical Vein Endothelial Cells

Several lines of evidence suggest an inhibitory role of dietary nucleotides (NTs) against oxidative stress and inflammation, which promote senescence in age-associated cardiovascular diseases. We sought to test whether the dietary NTs could retard the hydrogen peroxide (H2O2)-induced senescence of human umbilical vein endothelial cells (HUVECs) and to elucidate the efficiency of different NTs as well as the potential mechanism. Senescence was induced in HUVECs by 4 h exposure to 200 µM H2O2 and was confirmed using senescence-associated-β-galactosidase staining (SA-β-gal), cell viability, and Western blot analyses of p16INK4A and p21Waf1/Cip1 after 24 h administration of growth medium. We find that NTs retards oxidative stress-induced HUVECs senescence, as shown by a lower percentage of SA-β-gal-positive cells, lower expression of p16INK4A, and p21Waf1/Cip1 as well as higher cell viability. GMP100 was the most excellent in delaying HUVECs senescence, which was followed by the NTs mixture, NMN, CMP50, and UMP50/100, while AMP retards HUVECs senescence by specifically reducing p15INK4b expression. NTs all have significant anti-inflammatory effects; AMP and CMP were more prominent in restoring mitochondrial function, GMP and CMP were more competent at eliminating ROS and MDA, while AMP and UMP were more efficient at enhancing antioxidant enzyme activity. The role of the NTs mixture in retarding HUVECs senescence is full-scaled. These results stated that the mechanisms of NTs retarding HUVECs senescence could be related to its antioxidant and anti-inflammation properties promoting cell proliferation and protecting mitochondrial function activities.

Vascular Protective Role of Vitexicarpin Isolated from Vitex rotundifolia in Human Umbilical Vein Endothelial Cells

Inflammation ◽  
2011 ◽  
Vol 35 (2) ◽  
pp. 584-593 ◽  
Author(s):  
So Min Lee ◽  
Yun Jung Lee ◽  
Youn Chul Kim ◽  
Jin Sook Kim ◽  
Dae Gill Kang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Protective Role ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

scholarly journals Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Tianyi Li ◽  
Xianjing Song ◽  
Jichang Zhang ◽  
Lei Zhao ◽  
Yongfeng Shi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Protein Level ◽  
Umbilical Vein ◽  
Caspase 8 ◽  
Dependent Manner ◽  
Human Umbilical Vein ◽  
Qrt Pcr ◽  
Umbilical Vein Endothelial Cells

Oxidative stress induces endothelial cell apoptosis and promotes atherosclerosis development. MicroRNA-210 (miR-210) is linked with apoptosis in different cell types. This study aimed to investigate the role of miR-210 in human umbilical vein endothelial cells (HUVECs) under oxidative stress and to determine the underlying mechanism. HUVECs were treated with different concentrations of hydrogen peroxide (H2O2), and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ATP assay. To evaluate the role of miR-210 in H2O2-mediated apoptosis, gain-and-loss-of-function approaches were used, and the effects on apoptosis and reactive oxygen species (ROS) level were assayed using flow cytometry. Moreover, miR-210 expression was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and expression of the following apoptosis-related genes was assessed by qRT-PCR and Western blot at the RNA and protein level, respectively: caspase-8-associated protein 2 (CASP8AP2), caspase-8, and caspase-3. The results showed that H2O2induced apoptosis in HUVECs in a dose-dependent manner and increased miR-210 expression. Overexpression of miR-210 inhibited apoptosis and reduced ROS level in HUVECs treated with H2O2. Furthermore, miR-210 downregulated CASP8AP2 and related downstream caspases at protein level. Thus, under oxidative stress, miR-210 has a prosurvival and antiapoptotic effect on HUVECs by reducing ROS generation and downregulating the CASP8AP2 pathway.

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