Protective role of eugenol on arsenic induced oxidative DNA damage and modulatory effect of GSTO2 polymorphism

2018 ◽  
Vol 42 (5) ◽  
pp. e12565 ◽  
Author(s):  
Surbhi Bal ◽  
Anita Yadav ◽  
Neha Verma ◽  
Neeraj K. Aggarwal ◽  
Ranjan Gupta
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Protective Role

scholarly journals Role of Human NADPH Quinone Oxidoreductase (NQO1) in Oxygen-Mediated Cellular Injury and Oxidative DNA Damage in Human Pulmonary Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Rebecca Burke ◽  
Chun Chu ◽  
Guo-Dong Zhou ◽  
Vasanta Putluri ◽  
Nagireddy Putluri ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Viability ◽  
Oxidative Dna Damage ◽  
Oxidative Injury ◽  
Protective Role ◽  
Dna Lesions ◽  
Quinone Oxidoreductase ◽  
Pulmonary Cells ◽  
In Cells

Supplemental oxygen administration is frequently used in premature infants and adults with pulmonary insufficiency. NADPH quinone oxidoreductase (NQO1) protects cells from oxidative injury by decreasing reactive oxygen species (ROS). In this investigation, we tested the hypothesis that overexpression of NQO1 in BEAS-2B cells will mitigate cell injury and oxidative DNA damage caused by hyperoxia and that A-1221C single nucleotide polymorphism (SNP) in the NQO1 promoter would display altered susceptibility to hyperoxia-mediated toxicity. Using stable transfected BEAS-2B cells, we demonstrated that hyperoxia decreased cell viability in control cells (Ctr), but this effect was differentially mitigated in cells overexpressing NQO1 under the regulation of the CMV viral promoter, the wild-type NQO1 promoter (NQO1-NQO1), or the NQO1 promoter carrying the SNP. Interestingly, hyperoxia decreased the formation of bulky oxidative DNA adducts or 8-hydroxy-2 ′ -deoxyguanosine (8-OHdG) in Ctr cells. qPCR studies showed that mRNA levels of CYP1A1 and NQO1 were inversely related to DNA adduct formation, suggesting the protective role of these enzymes against oxidative DNA injury. In SiRNA experiments entailing the NQO1-NQO1 promoter, hyperoxia caused decreased cell viability, and this effect was potentiated in cells treated with CYP1A1 siRNA. We also found that hyperoxia caused a marked induction of DNA repair genes DDB2 and XPC in Ctr cells, supporting the idea that hyperoxia in part caused attenuation of bulky oxidative DNA lesions by enhancing nucleotide excision repair (NER) pathways. In summary, our data support a protective role for human NQO1 against oxygen-mediated toxicity and oxidative DNA lesions in human pulmonary cells, and protection against toxicity was partially lost in SNP cells. Moreover, we also demonstrate a novel protective role for CYP1A1 in the attenuation of oxidative cells and DNA injury. Future studies on the mechanisms of attenuation of oxidative injury by NQO1 should help in developing novel approaches for the prevention/treatment of ARDS in humans.

Role of oxidative DNA damage in the mechanism of fecapentaene 12 genotoxicity

1994 ◽  
Vol 15 (11) ◽  
pp. 2559-2566 ◽  
Author(s):  
T.M.C.M. de Kok ◽  
D.M.F.A. Pachen ◽  
J.M.S. van Maanen ◽  
M.V.M. Lafleur ◽  
E.J. Westmijze ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage

scholarly journals Potential role of oxidative DNA damage in the impact of PNPLA3 variant (rs 738409 C>G) in hepatocellular carcinoma risk

Hepatology ◽  
2014 ◽  
Vol 60 (3) ◽  
pp. 1110-1111 ◽  
Author(s):  
Emeric Limagne ◽  
Vanessa Cottet ◽  
Alexia Karen Cotte ◽  
Samia Hamza ◽  
Patrick Hillon ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Potential Role ◽  
The Impact ◽  
Carcinoma Risk

scholarly journals The Protective Role of Resveratrol against Arsenic Trioxide-Induced Cardiotoxicity

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Weiqian Zhang ◽  
Changming Guo ◽  
Ruifeng Gao ◽  
Ming Ge ◽  
Yanzhu Zhu ◽  
...  
Keyword(s):  
Arsenic Trioxide ◽  
Cardiac Dysfunction ◽  
Oxidative Dna Damage ◽  
Antioxidant Properties ◽  
Reactive Oxygen Species Generation ◽  
Protective Role ◽  
Natural Food ◽  
Heme Oxygenase 1 ◽  
Related Factor

Arsenic trioxide (As2O3) shows substantial anticancer activity in patients with acute promyelocytic leukemia (APL). Unfortunately, limiting the application of this effective agent to APL patients is severe cardiotoxicity. Resveratrol, the natural food-derived polyphenolic compound, is well known for its antioxidant properties and protects the cardiovascular system. But the potential role of resveratrol against As2O3in heart via nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) is unclear. The present study evaluated the effects of pretreatment with resveratrol and As2O3on oxidative stress and cardiac dysfunction in rat. In the present study, resveratrol decreased As2O3-induced reactive oxygen species generation, oxidative DNA damage, and pathological alterations. In addition, cardiac dysfunction parameters, intracellular calcium and arsenic accumulation, glutathione redox ratio, and cAMP deficiency levels were observed in As2O3-treated rats; these changes were attenuated by resveratrol. Furthermore, resveratrol significantly prohibited the downregulation of both Nrf2 and HO-1 gene expressions that were downregulated by As2O3, whereas resveratrol did not alter As2O3-induced nitric oxide formation. Thus, the protective role of resveratrol against As2O3-induced cardiotoxicity is implemented by the maintenance of redox homeostasis (Nrf2-HO-1 pathway) and facilitating arsenic efflux. Our findings suggest coadministration with resveratrol, and As2O3might provide a novel therapeutic strategy for APL.

scholarly journals The role of metal ion binding in the antioxidant mechanisms of reduced and oxidized glutathione in metal-mediated oxidative DNA damage

Metallomics ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 79-91 ◽  
Author(s):  
Elias O. U. Eteshola ◽  
Devin A. Haupt ◽  
Stephen I. Koos ◽  
Lee A. Siemer ◽  
Daniel L. Morris
Keyword(s):  
Dna Damage ◽  
Metal Ion ◽  
Oxidative Dna Damage ◽  
Ion Binding ◽  
Oxidized Glutathione ◽  
Metal Ion Binding ◽  
Reduced And Oxidized Glutathione ◽  
Antioxidant Mechanisms

GSH and GSSG appear to function as antioxidants against metal-mediated oxidative DNA damage by coordinating Fe(ii) and Cu(ii).

Protective role of thiols in carcinogen-induced DNA damage in rat liver

1986 ◽  
Vol 7 (10) ◽  
pp. 1621-1624 ◽  
Author(s):  
John Y.H. Chan ◽  
Daniel L. Stout ◽  
Frederick F. Becker
Keyword(s):  
Dna Damage ◽  
Rat Liver ◽  
Protective Role
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