Extraction and Biochemical Characterization of Peptidases from Giant Catfish Viscera by Aqueous Two-Phase System

2015 ◽  
Vol 39 (4) ◽  
pp. 429-438 ◽  
Author(s):  
Sunantha Ketnawa ◽  
Oscar Martinez-Alvarez ◽  
Soottawat Benjakul ◽  
Saroat Rawdkuen
Keyword(s):  
Biochemical Characterization ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Giant Catfish

Characterization of alcohol/salt aqueous two-phase system for optimal separation of gallic acids

2021 ◽  
Vol 131 (5) ◽  
pp. 537-542
Author(s):  
Hui Suan Ng ◽  
Phei Er Kee ◽  
Hip Seng Yim ◽  
Joo Shun Tan ◽  
Yin Hui Chow ◽  
...  
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Optimal Separation

scholarly journals Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system

2017 ◽  
Vol 133 ◽  
pp. 8-14 ◽  
Author(s):  
Maria Carolina de Albuquerque Wanderley ◽  
José Manoel Wanderley Duarte Neto ◽  
Wendell Wagner Campos Albuquerque ◽  
Daniela de Araújo Viana Marques ◽  
Carolina de Albuquerque Lima ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Phase System ◽  
Purification And Characterization ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Penicillium Sp

Extraction and purification of lipase from Enterococcus faecium MTCC5695 by PEG/phosphate aqueous-two phase system (ATPS) and its biochemical characterization

2016 ◽  
Vol 6 ◽  
pp. 19-27 ◽  
Author(s):  
Vrinda Ramakrishnan ◽  
Louella Concepta Goveas ◽  
Niranjan Suralikerimath ◽  
Chandrashekar Jampani ◽  
Prakash M. Halami ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Biochemical Characterization ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Extraction And Purification

scholarly journals Characterization of Amyloid β Fibrils with An Aqueous Two-Phase System: Implications of Fibril Formation

2010 ◽  
Vol 17 (0) ◽  
pp. 121-128 ◽  
Author(s):  
Toshinori SHIMANOUCHI ◽  
Naoya SHIMAUCHI ◽  
Keiichi NISHIYAMA ◽  
Huong Thi VU ◽  
Hisashi YAGI ◽  
...  
Keyword(s):  
Amyloid Β ◽  
Fibril Formation ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Purification and Characterization of Lipase Produced by Leuconostoc mesenteroides Subsp. mesenteroides ATCC 8293 Using an Aqueous Two-Phase System (ATPS) Composed of Triton X-100 and Maltitol

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1800 ◽  
Author(s):  
Nurfadhilah Eko Sukohidayat ◽  
Mohammad Zarei ◽  
Badlishah Baharin ◽  
Mohd Manap
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Process Characteristics ◽  
Sds Page ◽  
Ph Range ◽  
Lipase Purification ◽  
Triton X ◽  
First Time

Purification of lipase produced by L. mesenteroides subsp. mesenteroides ATCC 8293 was conducted for the first time using a novel aqueous two-phase system (ATPS) composed of Triton X-100 and maltitol. The partitioning of lipase was optimized according to several parameters including pH, temperature, and crude load. Results showed that lipase preferentially migrated to the Triton X-100 rich phase and optimum lipase partitioning was achieved in ATPS at TLL of 46.4% and crude load of 20% at 30 °C and pH 8, resulting in high lipase purification factor of 17.28 and yield of 94.7%. The purified lipase showed a prominent band on SDS-PAGE with an estimated molecular weight of 50 kDa. The lipase was stable at the temperature range of 30–60 °C and pH range of 6–11, however, it revealed its optimum activity at the temperature of 37 °C and pH 8. Moreover, lipase exhibited enhanced activity in the presence of non-ionic surfactants with increased activity up to 40%. Furthermore, results exhibited that metals ions such as Na+, Mg2+, K+ and Ca2+ stimulated lipase activity. This study demonstrated that this novel system could be potentially used as an alternative to traditional ATPS for the purification and recovery of enzymes since the purified lipase still possesses good process characteristics after undergoing the purification process.

Biotechnological purification of a β-fructofuranosidase (β-FFase) from Kita: Aqueous Two-phase system (PEG/Citrate) and Biochemical characterization

2021 ◽  
pp. 102070
Author(s):  
Juanize Matias da Silva Batista ◽  
Romero Marcos Pedrosa Brandão-Costa ◽  
Kethylen Barbara Barbosa Cardoso ◽  
Thiago Pajeú Nascimento ◽  
Wendell W.C. Albuquerque ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System
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