T richosanthes Cucumerina Fruit Extenuates Dyslipidemia, Protein Oxidation, Lipid Peroxidation and DNA Fragmentation in the liver of high-fat diet-fed rats

2014 ◽  
Vol 38 (5) ◽  
pp. 480-490 ◽  
Author(s):  
Taofeek O. Ajiboye ◽  
Sakirat A. Akinpelu ◽  
Hamdalat F. Muritala ◽  
Simiat M. Ogunbode ◽  
Abdulwasiu O. Adeleye ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Fragmentation ◽  
Protein Oxidation ◽  
High Fat Diet ◽  
High Fat

scholarly journals Strawberry Intake Ameliorates Oxidative Stress and Decreases GABA Levels Induced by High-Fat Diet in Frontal Cortex of Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 70 ◽  
Author(s):  
Cuauhtémoc Sandoval-Salazar ◽  
Cecilia Oviedo-Solís ◽  
Edmundo Lozoya-Gloria ◽  
Herlinda Aguilar-Zavala ◽  
Martha Solís-Ortiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Frontal Cortex ◽  
Protein Oxidation ◽  
High Fat Diet ◽  
High Fat ◽  
Gaba Concentration ◽  
Obese Rats ◽  
Lipid And Protein Oxidation

It has been proposed that there is a correlation between high-fat diet (HFD), oxidative stress and decreased γ-aminobutyric acid (GABA) levels, but this has not been thoroughly demonstrated. In the present study, we determined the effects of strawberry extract intake on the oxidative stress and GABA levels in the frontal cortex (FC) of obese rats. We observed that an HFD increased lipid and protein oxidation, and decreased GABA levels. Moreover, UV-irradiated strawberry extract (UViSE) decreased lipid peroxidation but not protein oxidation, whereas non-irradiated strawberry extract (NSE) reduced protein oxidation but not lipid peroxidation. Interestingly, NSE increased GABA concentration, whereas UViSE was not as effective. In conclusion, our results suggest that an HFD increases oxidative damage in the FC, whereas strawberry extract intake may ameliorate the disturbances associated with HFD-induced oxidative damage.

The Protective Effects of Probiotics on High Fat Diet-Induced Oxidative Damage Using a Comet Assay in Rats

2014 ◽  
Vol 998-999 ◽  
pp. 350-353
Author(s):  
Xiao Lian Chen ◽  
Lin Zhi Gong ◽  
Jian Xiong Xu
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Comet Assay ◽  
Oxidative Damage ◽  
Dna Fragmentation ◽  
High Fat Diet ◽  
Male Rats ◽  
Control Group ◽  
High Fat ◽  
Significant Difference

The objective of this study was to investigate the antioxidant capability and preventive effect of probiotics on high fat diet induced oxidative damage. Thirty male rats were randomly divided into three groups.The control group consumed a normal standard diet (5% fat, w:w), the high fat diet group received a high fat diet (20% fat, w:w), and probiotics group received a high fat diet supplemented with 2% probiotics. After 6 weeks, antioxidant capability of liver was determined and DNA fragmentation of liver cell was determined using a comet assay. The results showed that high fat diet could induce oxidative stress, shown as significant increases in lipid peroxidation and nitric oxide free radical, significant decrease in activities of superoxide dismutase and glutathione peroxidase, the percentage of DNA fragmentation was markedly increased, compared with control group. These alterations were significantly reversed in probiotics supplemented group and had no significant difference in antioxidant capability, lipid peroxidation and DNA damage compared with control group. Present observation indicated the probiotics had antioxidant property, which could scavenge free radical and enhance antioxidant defense system, and had the positive protective function on oxidative damage.

scholarly journals A High Fat Diet Inhibits δ-Aminolevulinate Dehydratase and Increases Lipid Peroxidation in Mice (Mus musculus)

2003 ◽  
Vol 133 (7) ◽  
pp. 2165-2170 ◽  
Author(s):  
Vanderlei Folmer ◽  
Júlio C. M. Soares ◽  
Diogo Gabriel ◽  
João B. T. Rocha
Keyword(s):  
Lipid Peroxidation ◽  
Mus Musculus ◽  
High Fat Diet ◽  
High Fat ◽  
Aminolevulinate Dehydratase

scholarly journals Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
O. Merino ◽  
R. Sánchez ◽  
B. M. Gregorio ◽  
F. J. Sampaio ◽  
J. Risopatrón
Keyword(s):  
Vitamin D ◽  
Dna Fragmentation ◽  
High Fat Diet ◽  
Control Diet ◽  
Sperm Function ◽  
Sprague Dawley ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Sprague Dawley Rats ◽  
The Impact

Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD-). Discussion on the impact of obesity/VD- on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD- on viability and plasma membrane integrity (PMI), superoxide anion (O2-) level, and DNA fragmentation (DNAfrag) in sperm Sprague-Dawley rats. The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D (C/VD+), G2: control diet without vitamin D (C/VD-), G3: high-fat diet with vitamin D (HF/VD+), and G4: high-fat diet without vitamin D (HF/VD-). Sperm function parameters were analyzed by flow cytometry. PMI percentages and O2- levels were not affected by any of the diets. DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4). Our results allow us to point out that diet-induced obesity and VD- produce greater damage in DNA sperm of rats. The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa.

Lactobacilli modulated AMPK activity and prevented telomere shortening in ageing rats

2019 ◽  
Vol 10 (8) ◽  
pp. 883-892
Author(s):  
L.C. Lew ◽  
Y.Y. Hor ◽  
M.H. Jaafar ◽  
A.S.Y. Lau ◽  
J.S. Ong ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Rat Model ◽  
High Fat Diet ◽  
Lactobacillus Reuteri ◽  
Plasma Lipid ◽  
Dependent Manner ◽  
Sprague Dawley ◽  
High Fat ◽  
Age Related ◽  
Plasma Lipid Peroxidation

This study aimed to evaluate the anti-ageing effects of different strains of lactobacilli putative probiotics on an ageing rat model as induced by D-galactose and a high fat diet. Male Sprague-Dawley rats were fed with high fat diet (54% kcal fat) and injected with D-galactose daily for 12 weeks to induce ageing. The effects of putative probiotic strains on age-related impairment such as telomere length, plasma lipid peroxidation, hepatic 5’adenosine monophosphate-activated protein kinase (AMPK) expression, as well as endurance performance were evaluated. Administration of statin, Lactobacillus plantarum DR7 (LP-DR7), Lactobacillus fermentum DR9 (LF-DR9), and Lactobacillus reuteri 8513d (LR-8513d) significantly reduced the shortening of telomere and increased the expression of AMPK subunit-α1 (P<0.05). Plasma lipid peroxidation was lower (P<0.05) in groups administered with statin and LF-DR9 as compared to the control. AMPK subunit-α2 was elevated in rats administered with LP-DR7 as compared to the control (P<0.05). Using an in vivo ageing rat model, the current study has illustrated the potentials of lactobacilli putative probiotics in alleviation of age-related impairment in a strain-dependent manner.

scholarly journals Jaboticaba (Myrciaria jaboticaba (Vell.) Berg.) peel improved triglycerides excretion and hepatic lipid peroxidation in high-fat-fed rats

2013 ◽  
Vol 26 (5) ◽  
pp. 571-581 ◽  
Author(s):  
Ângela Giovana Batista ◽  
Sabrina Alves Lenquiste ◽  
Carolin Moldenhauer ◽  
Juliana Teixeira Godoy ◽  
Soely Maria Pissini Machado Reis ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
High Fat Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Hepatic Lipid ◽  
Fecal Lipid ◽  
Freeze Dried ◽  
Triglyceride Content ◽  
Diet Control ◽  
Hepatic Lipid Peroxidation

OBJECTIVE: The aim of this study was to evaluate the influence of high-fat diets with 1%, 2%, and 4% freeze-dried jaboticaba peel on the serum, liver, and fecal lipid profile of obese rats. METHODS: Thirty male Sprague-Dawley rats were divided into 5 groups. Obesity was induced in four groups using a high-fat diet (35% lipids). One group was used as a high-fat diet control (High-fat group - HF). The other three high-fat-diet groups were given 1%, 2%, and 4% freeze-dried jaboticaba peel (High-Fat Jaboticaba - HFJ1, HFJ2, and HFJ4, respectively) in the last 40 experimental days. Blood and the liver were collected after 70 days of treatment and feces were collected in the last experimental week. Total cholesterol, triglycerides, and lipids were measured in the serum, liver, and dried feces. ffer in the experimental groups. HFJ2 group had the highest hepatic and fecal lipid contents compared with the group fed a diet with normal fat content (N), but low hepatic lipid peroxidation. HFJ4 group had the highest mean hepatic and fecal cholesterol levels. Hepatic triglyceride levels did not differ among the groups, and groups HFJ1 and HFJ4 presented the highest fecal triglyceride content. CONCLUSION: The amounts of jaboticaba peel used by this study did not protect against hepatic steatosis or undesired levels of other studied lipids, but it did increase fecal triglycerides. Lipid peroxidation in the liver decreased in the HFJ2 group.

Protective effects of phyllanthin, a lignan from Phyllanthus amarus, against progression of high fat diet induced metabolic disturbances in mice

RSC Advances ◽  
2016 ◽  
Vol 6 (63) ◽  
pp. 58343-58353 ◽  
Author(s):  
Sneha Jagtap ◽  
Pragyanshu Khare ◽  
Priyanka Mangal ◽  
Kanthi Kiran Kondepudi ◽  
Mahendra Bishnoi ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Lipid Peroxidation ◽  
Glucose Metabolism ◽  
High Fat Diet ◽  
Phyllanthus Amarus ◽  
Protective Effects ◽  
Liver Inflammation ◽  
High Fat ◽  
Metabolic Disturbances ◽  
Induced Changes

Phyllanthin delayed the progression of high fat diet induced changes affecting lipid and glucose metabolism such as adiposity, hypertriglyceridemia, fatty liver, inflammation, lipid peroxidation and insulin resistance.

scholarly journals Haloperidol increases hepatic lipid peroxidation promoted by high-fat diet in rats

2020 ◽  
Vol 9 (2) ◽  
pp. e148922153
Author(s):  
Ilson Dias da Silveira ◽  
Daniel Henrique Roos ◽  
Andréia Caroline Fernandes Salgueiro ◽  
Vanderlei Folmer ◽  
João Batista Teixeira da Rocha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Negative Correlation ◽  
High Fat Diet ◽  
Control Diet ◽  
Abdominal Fat ◽  
Fat Content ◽  
High Fat ◽  
Intracellular Magnesium ◽  
Hepatic Lipid ◽  
Hepatic Lipid Peroxidation

This study aimed to evaluate the effects of the treatment with haloperidol (HAL) associated with a high-fat diet (HF) on hepatic and renal damage, intracellular magnesium (Mg2+) levels, and abdominal fat content. Young male Wistar rats were fed with high-fat diet or control diet during 48 weeks and, at the 24-week, part of animals began to be co-treated with HAL (1 mg/Kg/day intramuscularly). After 4 weeks of the drug administration, the livers and kidneys were removed for analyses. The results showed that HF diet significantly increased lipid peroxidation in the hepatic tissue of treated animals, when compared to animals treated with control diet (P<0.05). Moreover, HF associated with HAL further increased the hepatic lipid peroxidation levels (P<0.05). In contrast, HF and/or HAL did not promote significant changes in renal lipid peroxidation levels. We also found a negative correlation between intracellular Mg2+ levels and abdominal fat content among all animals. In conclusion, the data presented suggest adverse interactions between HAL and HF on liver. Furthermore, the negative correlation between the intracellular Mg2+ levels and the abdominal fat accumulation suggest a possible involvement of Mg2+ in the metabolic syndrome development associated with a HF diet.

scholarly journals Fermented Miso with Adzuki Beans or Black Soybeans Decreases Lipid Peroxidation and Serum Cholesterol in Mice fed a High-Fat Diet

2015 ◽  
Vol 3 (3) ◽  
pp. 131-137 ◽  
Author(s):  
Shan Wu ◽  
Shuo Feng ◽  
ZhaoHong Ci ◽  
Chengyu Jiang ◽  
Yang Cui ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Serum Cholesterol ◽  
High Fat Diet ◽  
High Fat

Lipid droplets are both highly oxidized and Plin2-covered in hepatocytes of diet-induced obese mice

2020 ◽  
Vol 45 (12) ◽  
pp. 1368-1376
Author(s):  
Diego Nocetti ◽  
Alejandra Espinosa ◽  
Francisco Pino-De la Fuente ◽  
Camila Sacristán ◽  
José Luis Bucarey ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
High Fat Diet ◽  
Lipid Droplets ◽  
Control Diet ◽  
Isolated Hepatocytes ◽  
High Fat ◽  
Nonalcoholic Fatty Liver ◽  
Adult Mice ◽  
Obesity Status ◽  
Intrahepatic Lipid

Chronic high-fat diet feeding is associated with obesity and accumulation of fat in the liver, leading to the development of insulin resistance and nonalcoholic fatty liver disease. This condition is characterized by the presence of a high number of intrahepatic lipid droplets (LDs), with changes in the perilipin pattern covering them. This work aimed to describe the distribution of perilipin (Plin) 2, an LD-associated protein involved in neutral lipid storage, and Plin5, which favors lipid oxidation in LD, and to evaluate lipid peroxidation through live-cell visualization using the lipophilic fluorescent probe C11-BODIPY581/591 in fresh hepatocytes isolated from mice fed a high-fat diet (HFD). Male C57BL/6J adult mice were divided into control and HFD groups and fed with a control diet (10% fat, 20% protein, and 70% carbohydrates) or an HFD (60% fat, 20% protein, and 20% carbohydrates) for 8 weeks. The animals fed the HFD showed a significant increase of Plin2 in LD of hepatocytes. LD from HFD-fed mice have a stronger lipid peroxidation level than control hepatocytes. These data provide evidence that obesity status is accompanied by a higher degree of lipid peroxidation in hepatocytes, both in the cytoplasm and in the fats stored inside the LD. Novelty Our study shows that lipid droplets from isolated hepatocytes in HFD-fed mice have a stronger lipid peroxidation level than control hepatocytes. C11-BODIPY581/591 is a useful tool to measure the initial level of intracellular lipid peroxidation in single isolated hepatocytes. Perilipins pattern changes with HFD feeding, showing an increase of Plin2 covering lipid droplets.

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