Insights into the functional role of grass carp IL ‐8 in head kidney leukocytes: pro‐inflammatory effects and signaling mechanisms

2021 ◽  
Author(s):  
Minghui Zhao ◽  
Yazhen Liu ◽  
Yajun Gao ◽  
Xinyan Wang ◽  
Hong Zhou ◽  
...  
Keyword(s):  
Grass Carp ◽  
Functional Role ◽  
Head Kidney ◽  
Signaling Mechanisms

Kisspeptin induction of somatolactin-α release in goldfish pituitary cells: functional role of cAMP/PKA-, PLC/PKC-, and Ca2+/calmodulin-dependent cascades

2014 ◽  
Vol 307 (10) ◽  
pp. E872-E884 ◽  
Author(s):  
Quan Jiang ◽  
Mulan He ◽  
Wendy K. W. Ko ◽  
Anderson O. L. Wong
Keyword(s):  
Protein Kinase ◽  
Functional Role ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Pituitary Hormone ◽  
Pituitary Cells ◽  
Signaling Mechanisms ◽  
Intracellular Ca ◽  
Subsequent Activation

Although the importance of kisspeptin in the pituitary is firmly established, the signaling mechanisms for the pituitary actions of kisspeptin are still largely unknown. Somatolactin (SL), a member of the growth hormone (GH)/prolactin (PRL) family, is a pituitary hormone with pleiotropic functions in fish, but its regulation by kisspeptin has not been examined. To investigate the functional role of kisspeptin in SL regulation, expression of two paralogues of goldfish Kiss1 receptors (Kiss1ra and Kiss1rb) were confirmed in immunoidentified SLα but not SLβ cells isolated by RT-PCR coupled with laser capture microdissection. In goldfish pituitary cells prepared from neurointermediate lobe (NIL), synthetic goldfish Kiss decapeptides (gKiss1-10 and gKiss2-10) could increase SLα release. Consistent with the lack of Kiss1r expression in SLβ cells, SLβ release was not altered by kisspeptin stimulation. In parallel experiments, goldfish gKiss1-10 could elevate cyclic adenosine monophosphate (cAMP) production, upregulate protein kinase A (PKA) and protein kinase C (PKC) activities, and trigger a rapid rise in intracellular Ca2+ levels in goldfish NIL cells. Using a pharmacological approach, cAMP/PKA and phospholipase C (PLC)/PKC pathways and subsequent activation of Ca2+/calmodulin (CaM)-dependent cascades were shown to be involved in SLα release induced by gKiss1-10. Apparently, the Ca2+-dependent cascades were triggered by extracellular Ca2+ entry via voltage-sensitive Ca2+ channels and mobilization of inositol trisphosphate-sensitive intracellular Ca2+ stores. Our results demonstrate that gKiss1-10 can act directly at the pituitary level to trigger SLα release via a complex network of post-receptor signaling mechanisms.

scholarly journals Protein Phosphatase PP1 Negatively Regulates IRF3 in Response to GCRV Infection in Grass Carp (Ctenopharyngodon idella)

2021 ◽  
Vol 11 ◽  
Author(s):  
Xudong Hu ◽  
Bing Wang ◽  
Haohao Feng ◽  
Man Zhou ◽  
Yusheng Lin ◽  
...  
Keyword(s):  
Grass Carp ◽  
Protein Phosphatase ◽  
Head Kidney ◽  
Negative Regulator ◽  
Ctenopharyngodon Idella ◽  
Distribution Analysis ◽  
Cell Functions ◽  
Grass Carp Ctenopharyngodon Idella

Protein phosphatase-1 (PP1) has an important role in many cell functions, such as cell differentiation, development, immune response and tumorigenesis. However, the specific role of PP1 in the antiviral response in fish remains to be elucidated. In this study, the PPP1R3G homolog was identified in the grass carp (Ctenopharyngodon idella) and its role in defence against the GCRV infection was investigated. Phylogenetic analysis demonstrated that CiPPP1R3G clustered with homologues from other teleosts. Temporal expression analysis in vivo revealed that the expression level of CiPPP1R3G was significantly up-regulated in response to GCRV infection in grass carps, especially in the intestine and head-kidney. Cellular distribution analysis revealed that CiPPP1R3G was located in the nucleus and cytoplasm. Overexpression of CiPPP1R3G significantly negatively regulated the expression of CiIRF3, thus inhibiting its activation. In summary, we systematically analyzed the PPP1R3G gene in grass carp and illustrated its function as a negative regulator in the anti-GCRV immune responses.

Expression and functional role of c-Kit and erbB2 in human hepatoblastoma.

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
B Steiger ◽  
I Leuschner ◽  
D Denkhaus ◽  
D von Schweinitz ◽  
T Pietsch
Keyword(s):  
Functional Role
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