DNA metabarcoding for dietary analysis of Holland's carp ( Spinibarbus hollandi ) to evaluate the threat to native fishes in Taiwan

2021 ◽  
Author(s):  
Chien‐I Lee ◽  
Feng‐Yu Wang ◽  
Min‐Yun Liu ◽  
Tak‐Kei Chou ◽  
Te‐Yu Liao
Keyword(s):  
Dietary Analysis ◽  
Dna Metabarcoding ◽  
Native Fishes

Metabarcoding demonstrates dietary niche partitioning in two coexisting blackfish species

2020 ◽  
Vol 71 (4) ◽  
pp. 512
Author(s):  
Gavin N. Rees ◽  
Michael E. Shackleton ◽  
Garth O. Watson ◽  
Georgia K. Dwyer ◽  
Rick J. Stoffels
Keyword(s):  
Fish Species ◽  
Niche Partitioning ◽  
Significant Degree ◽  
Frequency Of Occurrence ◽  
Dietary Analysis ◽  
Terrestrial Invertebrates ◽  
Dna Metabarcoding ◽  
Total Diet ◽  
Dietary Niche

A problem for fisheries ecologists who carry out dietary analysis on their specimens is dealing with contents that are difficult to identify, particularly when the contents comprise digested prey. We used a DNA metabarcoding approach to determine the diets of two co-occurring black fish species (Gadopsis bispinosus and Gadopsis marmoratus) to circumvent any issues with trying to apply microscopic methods to identify diets. We examined the frequency of occurrence of taxa across all specimens and the proportion that taxa contributed to total diet. In this way we hoped to demonstrate that a DNA-based method could resolve dietary differences of coexisting taxa. We showed that 10 macroinvertebrate taxa dominated the diets of both species and, of these, 7 occurred in all specimens of both taxa, indicating they were an important component of the diet of both species. Twelve taxa were present only in the G. bispinosus diet and four of those were terrestrial invertebrates; six taxa were found only in G. marmoratus. Our DNA-based approach to examine the taxa in the guts of two co-existing Gadopsis species provided sufficient resolution to show a significant degree of dietary partitioning.

scholarly journals Using multi-marker DNA metabarcoding to reveal the diet of a scarce woodland bird

2021 ◽  
Author(s):  
Ewan Stenhouse ◽  
Paul Bellamy ◽  
Will Kirby ◽  
Ian Vaughan ◽  
Lorna Drake ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Bird Species ◽  
Dietary Analysis ◽  
Multiple Resources ◽  
Operational Taxonomic Units ◽  
Faecal Samples ◽  
Dna Metabarcoding ◽  
Dietary Plasticity ◽  
Invertebrate Taxon ◽  
The Uk

Understanding the role diet plays in the structure of food webs is vital, and dietary knowledge is key for conservation management success. There is limited knowledge of the diets of woodland bird species, due largely to difficulties in accurately identifying plant and invertebrate taxa being consumed. Here, we show the effectiveness of multi-marker faecal metabarcoding to provide the most in-depth dietary analysis of a generalist passerine, the Hawfinch (Coccothraustes coccothraustes, Linnaeus), to date. Faecal samples were obtained from 2016-2019 from Hawfinch populations prior to and during the breeding season throughout the UK. DNA was extracted from 263 samples and amplified using Internal Transcribed Spacer 2 (ITS2) and cytochrome C oxidase subunit I (COI) barcodes. Using high-throughput sequencing (HTS), we identified 49 and 97 ITS2 and COI zero radius operational taxonomic units (zOTUs) respectively which equated to reputed dietary items. The herbivorous element of Hawfinch diet was dominated by naturally occurring taxa such as beech (Fagus sylvatica, Linnaeus), hornbeam (Carpinus betulus, Linnaeus) and oak (Quercus sp., Linnaeus). The most taxon rich and commonly recorded invertebrate taxon identified was Lepidoptera. We found Hawfinch diet varied spatially, as well as between sexes. Hawfinch showed broad dietary plasticity and utilised multiple resources within their foraging environments. Our study shows the potential of multi-marker DNA metabarcoding to reveal subtle dietary differences, but also highlights the challenges of studying omnivorous species using metabarcoding methods.

COMPARATIVE STUDY BETWEEN FISH COLLECTION BY NATIONAL CENSUS ON RIVER ENVIRONMENT AND ENVIRONMENTAL DNA METABARCODING

2018 ◽  
Vol 74 (5) ◽  
pp. I_415-I_420 ◽  
Author(s):  
Yoshihisa AKAMATSU ◽  
Takayoshi TSUZUKI ◽  
Ryota YOKOYAMA ◽  
Yayoi FUNAHASHI ◽  
Munehiro OHTA ◽  
...  
Keyword(s):  
Comparative Study ◽  
Environmental Dna ◽  
Dna Metabarcoding ◽  
Fish Collection

Environmental DNA for functional diversity

2018 ◽  
Author(s):  
Pierre Taberlet ◽  
Aurélie Bonin ◽  
Lucie Zinger ◽  
Eric Coissac
Keyword(s):  
Functional Groups ◽  
Functional Diversity ◽  
Environmental Dna ◽  
Soil Organisms ◽  
Meaningful Information ◽  
Dna Metabarcoding ◽  
Pros And Cons ◽  
Target Community

Chapter 10 “Environmental DNA for functional diversity” discusses the potential of environmental DNA to assess functional diversity. It first focuses on DNA metabarcoding and discusses the extent to which this approach can be used and/or optimized to retrieve meaningful information on the functions of the target community. This knowledge usually involves coarsely defined functional groups (e.g., woody, leguminous, graminoid plants; shredders or decomposer soil organisms; pathogenicity or decomposition role of certain microorganisms). Chapter 10 then introduces metagenomics and metatranscriptomics approaches, their advantages, but also the challenges and solutions to appropriately sampling, sequencing these complex DNA/RNA populations. Chapter 10 finally presents several strategies and software to analyze metagenomes/metatranscriptomes, and discusses their pros and cons.

Environmental DNA

2018 ◽  
Author(s):  
Pierre Taberlet ◽  
Aurélie Bonin ◽  
Lucie Zinger ◽  
Eric Coissac
Keyword(s):  
Graduate Students ◽  
Feeding Habits ◽  
Environmental Dna ◽  
Research Field ◽  
Background Information ◽  
Ecological Processes ◽  
Biodiversity Research ◽  
Dna Metabarcoding ◽  
Standard Information

Environmental DNA (eDNA), i.e. DNA released in the environment by any living form, represents a formidable opportunity to gather high-throughput and standard information on the distribution or feeding habits of species. It has therefore great potential for applications in ecology and biodiversity management. However, this research field is fast-moving, involves different areas of expertise and currently lacks standard approaches, which calls for an up-to-date and comprehensive synthesis. Environmental DNA for biodiversity research and monitoring covers current methods based on eDNA, with a particular focus on “eDNA metabarcoding”. Intended for scientists and managers, it provides the background information to allow the design of sound experiments. It revisits all steps necessary to produce high-quality metabarcoding data such as sampling, metabarcode design, optimization of PCR and sequencing protocols, as well as analysis of large sequencing datasets. All these different steps are presented by discussing the potential and current challenges of eDNA-based approaches to infer parameters on biodiversity or ecological processes. The last chapters of this book review how DNA metabarcoding has been used so far to unravel novel patterns of diversity in space and time, to detect particular species, and to answer new ecological questions in various ecosystems and for various organisms. Environmental DNA for biodiversity research and monitoring constitutes an essential reading for all graduate students, researchers and practitioners who do not have a strong background in molecular genetics and who are willing to use eDNA approaches in ecology and biomonitoring.

scholarly journals Pollen DNA metabarcoding identifies regional provenance and high plant diversity in Australian honey

2021 ◽  
Author(s):  
Liz Milla ◽  
Kale Sniderman ◽  
Rose Lines ◽  
Mahsa Mousavi‐Derazmahalleh ◽  
Francisco Encinas‐Viso
Keyword(s):  
Plant Diversity ◽  
Dna Metabarcoding

Environmental DNA metabarcoding as a useful tool for evaluating terrestrial mammal diversity in tropical forests

2021 ◽  
Author(s):  
José Luis Mena ◽  
Hiromi Yagui ◽  
Vania Tejeda ◽  
Emilio Bonifaz ◽  
Eva Bellemain ◽  
...  
Keyword(s):  
Tropical Forests ◽  
Environmental Dna ◽  
Terrestrial Mammal ◽  
Dna Metabarcoding ◽  
Mammal Diversity

scholarly journals The influence of intraspecific sequence variation during DNA metabarcoding: A case study of eleven fungal species

2021 ◽  
Author(s):  
Eva Lena F. Estensmo ◽  
Sundy Maurice ◽  
Luis Morgado ◽  
Pedro M. Martin‐Sanchez ◽  
Inger Skrede ◽  
...  
Keyword(s):  
Sequence Variation ◽  
Fungal Species ◽  
Dna Metabarcoding
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