Freshening effect on the osmotic response of the A ntarctic spiny plunderfish Harpagifer antarcticus

2021 ◽  
Author(s):  
Luis Vargas‐Chacoff ◽  
Francisco Dann ◽  
Kurt Paschke ◽  
Ricardo Oyarzún‐Salazar ◽  
Daniela Nualart ◽  
...  
Keyword(s):  
Osmotic Response

scholarly journals Osmotic response of Dotilla fenestrata (sand bubbler crab) exposed to combined water acidity and varying metal (Cd and Pb)

Heliyon ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. e06763
Author(s):  
Babatunde Adeleke ◽  
Deborah Robertson-Andersson ◽  
Gan Moodley
Keyword(s):  
Osmotic Response ◽  
Water Acidity

scholarly journals Role for the Ran Binding Protein, Mog1p, in Saccharomyces cerevisiae SLN1-SKN7 Signal Transduction

2004 ◽  
Vol 3 (6) ◽  
pp. 1544-1556 ◽  
Author(s):  
Jade Mei-Yeh Lu ◽  
Robert J. Deschenes ◽  
Jan S. Fassler
Keyword(s):  
Signal Transduction ◽  
Transcription Factor ◽  
Osmotic Stress ◽  
Kinase Activity ◽  
Mitogen Activated Protein Kinase ◽  
Osmotic Response ◽  
Mitogen Activated Protein ◽  
Kinase Pathway

ABSTRACT Yeast Sln1p is an osmotic stress sensor with histidine kinase activity. Modulation of Sln1 kinase activity in response to changes in the osmotic environment regulates the activity of the osmotic response mitogen-activated protein kinase pathway and the activity of the Skn7p transcription factor, both important for adaptation to changing osmotic stress conditions. Many aspects of Sln1 function, such as how kinase activity is regulated to allow a rapid response to the continually changing osmotic environment, are not understood. To gain insight into Sln1p function, we conducted a two-hybrid screen to identify interactors. Mog1p, a protein that interacts with the yeast Ran1 homolog, Gsp1p, was identified in this screen. The interaction with Mog1p was characterized in vitro, and its importance was assessed in vivo. mog1 mutants exhibit defects in SLN1-SKN7 signal transduction and mislocalization of the Skn7p transcription factor. The requirement for Mog1p in normal localization of Skn7p to the nucleus does not fully account for the mog1-related defects in SLN1-SKN7 signal transduction, raising the possibility that Mog1p may play a role in Skn7 binding and activation of osmotic response genes.

scholarly journals Osmotic Response Element Enhancer Activity

1999 ◽  
Vol 274 (29) ◽  
pp. 20185-20190 ◽  
Author(s):  
Varsha Nadkarni ◽  
Kenneth H. Gabbay ◽  
Kurt M. Bohren ◽  
David Sheikh-Hamad
Keyword(s):  
Enhancer Activity ◽  
Response Element ◽  
Osmotic Response

Responses of growth cones to changes in osmolality of the surrounding medium

1991 ◽  
Vol 98 (4) ◽  
pp. 507-515
Author(s):  
D. Bray ◽  
N.P. Money ◽  
F.M. Harold ◽  
J.R. Bamburg
Keyword(s):  
Hydrostatic Pressure ◽  
Growth Cone ◽  
Culture Medium ◽  
Vapor Pressure Deficit ◽  
Surrounding Medium ◽  
Culture Fluid ◽  
Short Term ◽  
Area Reduction ◽  
Osmotic Response ◽  
Upper Limits

The possible involvement of osmotically generated hydrostatic pressure in driving actin-rich extensions of the cell surface was examined using cultures of chick neurons. Estimation of the excess internal osmotic pressure of chick neural tissue by vapor pressure deficit osmometry, and of the excess internal hydrostatic pressure in cultured chick neurons using a calibrated pressure pipette, gave upper limits of 10 mosM and 0.1 atmosphere (1 atmosphere = 101325 Pa), respectively. Increases in the osmolality of the medium surrounding cultured neurons by addition of sucrose, mannitol or polyethylene glycol by amounts that should eliminate any internal pressure not only failed to arrest the growth of filopodia but caused them to increase in length up to twofold in 3–5 min. Lamellipodia remained unchanged following hyperosmotic shifts of 20 mosM, but higher levels caused a small decrease in area. Reduction of osmolality by the addition of water to the culture fluid down to 50% of its normal value failed to show any detectable change in either filopodial length or lamellipodia area. These observations argue against an osmotic mechanism for growth cone extension and show that the growth of filopodia, in particular, is unlikely to be driven by osmotically generated hydrostatic pressure. In contrast to the short-term effects on growth cone morphology, the slower elongation of the neuritic cylinder showed a consistent osmotic response. Growth rates were reduced following addition of osmolytes and increased in rate (as much as sixfold) following addition of water to the culture medium.(ABSTRACT TRUNCATED AT 250 WORDS)

pH effect on osmotic response of collecting tubules to vasopressin and 8-CPT-cAMP

1983 ◽  
Vol 245 (2) ◽  
pp. F188-F197 ◽  
Author(s):  
M. Lorenzen ◽  
A. Taylor ◽  
E. E. Windhager
Keyword(s):  
Ph Effect ◽  
T Test ◽  
Osmotic Response ◽  
Collecting Tubule ◽  
Before And After ◽  
Luminal Ph ◽  
Collecting Tubules ◽  
Induced Changes ◽  
Paired T Test ◽  
Rabbit Cortical Collecting Tubule

The effect of peritubular and luminal pH changes on hydraulic conductance, (Lp, 10(-7) cm X s-1 X atm-1) in the isolated perfused rabbit cortical collecting tubule (CCT) was tested at 37 degrees C before and after administration of 20 microU/ml vasopressin or 10(-4) M 8-[p-chlorophenylthio]-adenosine cyclic monophosphate (8-CPT-cAMP). In vasopressin experiments when bath pH was changed from 7.58 to 7.16 or from 7.58 to 6.70, mean Lp decreased from 249 +/- 32 to 199 +/- 23 (n = 5; P less than 0.01) and from 231 +/- 38 to 201 +/- 36 (n = 5; NS), respectively. In contrast, in 8-CPT-cAMP experiments when bath [HCO3] was kept constant while CO2 was elevated the hydroosmotic response was increased. Using 2.5 mM HCO3, Lp at 0.4% CO2 was 275 +/- 15 and at 6% CO2 it was 352 +/- 50 (n = 4; paired t test; P less than 0.05). At 8.5 and 21.5 mM HCO3 raising CO2 from 2 to 13% and from 4 to 32% increased Lp from 237 +/- 71 to 410 +/- 32 (n = 4; paired t test; P less than 0.05) and from 282 +/- 45 to 449 +/- 63 (n = 6; paired t test; P less than 0.001), respectively. Reducing luminal pH from 7.40 to 5.40 had no effect on either vasopressin- or cAMP-induced changes in Lp. Accordingly, lowering the bath pH by increasing the PCO2 at constant [HCO3] markedly stimulates the response to 8-CPT-cAMP, whereas lowering the bath pH by reducing [HCO3] inhibits the vasopressin response.

scholarly journals Cloning, genomic organization, and osmotic response of the aldose reductase gene.

1994 ◽  
Vol 91 (22) ◽  
pp. 10742-10746 ◽  
Author(s):  
J. D. Ferraris ◽  
C. K. Williams ◽  
B. M. Martin ◽  
M. B. Burg ◽  
A. Garcia-Perez
Keyword(s):  
Aldose Reductase ◽  
Genomic Organization ◽  
Osmotic Response ◽  
Reductase Gene

Osmotic response of oocytes using a microscope diffusion chamber: A preliminary study comparing murine and human ova

Cryobiology ◽  
1988 ◽  
Vol 25 (6) ◽  
pp. 495-501 ◽  
Author(s):  
A. Bernard ◽  
J.J. McGrath ◽  
B.J. Fuller ◽  
D. Imoedemhe ◽  
R.W. Shaw
Keyword(s):  
Diffusion Chamber ◽  
Osmotic Response ◽  
Preliminary Study

Effect of Cell-to-Surface Interaction on Freeze Tolerance and Osmotic Response of Cells

2008 ◽  
Author(s):  
Takashi Yoshimori ◽  
Masaki Fukagawa ◽  
Hiroshi Takamatsu
Keyword(s):  
Cell Suspension ◽  
Cell Injury ◽  
Freeze Tolerance ◽  
Surface Interaction ◽  
Artificial Organs ◽  
Freezing Injury ◽  
Elevated Concentration ◽  
Freeze Thaw ◽  
Osmotic Response ◽  
The Difference

Cryopreservation of tissues and organs, including artificial organs, could be one of the important steps in the medical service that brings the progress in the tissue engineering to realization. In this case, high viability of cryopreserved cells is critical to recovery after transplantation. In contrast, in the cryosurgery, which is expected to expand its application as a minimally invasive treatment of cancer, malignant cells should be destructed completely to prevent from recurrence. The appropriate freeze-thaw protocol is therefore needed to be established for cryopreservation or cryosurgery depending on specific type of tissues and organs. Although it is determined empirically, the underlying mechanism of cell injury by freezing has been explored for a long time to give a scientific basis of the process. The experiments with a cell suspension showed that the cell injury during slow freezing to a relatively higher sub-zero temperature was attributed to the mechanical stress from the extracellular ice, while the effect of elevated concentration of solutes became more crucial to cell damage at lower temperatures [1]. However, there are some studies that indicates the difference in the freeze tolerance between cell suspensions and attached monolayers, some of which indicated higher susceptibility of monolayers to freezing than cell suspension [2] and the other suggested reverse [3,4]. The goal of our study is thus to validate the difference in freezing injury between isolated cells and tissues that are more important in aforementioned applications and clarify the mechanism. We used cells adhered to a surface as a first simple model of cells in tissues. The cells adhered on a surface at low number density were used to highlight the effect of cell-to-surface interaction without cell-to-cell interactions. In the present study we first demonstrate that the survival of cells adhered on a surface is lower than those in the suspension after a freeze-thaw manipulation. Then the osmotic response to concentration increase was examined to clarify if the extent of dehydration is different between these two types of cells. The cells were observed by a laser confocal scanning microscope that allows real-time 3-D observation.

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