DNA barcoding of freshwater fishes and the development of a quantitative qPCR assay for the species-specific detection and quantification of fish larvae from plankton samples

2014 ◽  
Vol 85 (2) ◽  
pp. 307-328 ◽  
Author(s):  
W. K. W. Loh ◽  
P. Bond ◽  
K. J. Ashton ◽  
D. T. Roberts ◽  
I. R. Tibbetts
Keyword(s):  
Dna Barcoding ◽  
Fish Larvae ◽  
Freshwater Fishes ◽  
Qpcr Assay ◽  
Specific Detection ◽  
Species Specific ◽  
Detection And Quantification

Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea

2019 ◽  
Vol 510 ◽  
pp. 31-45 ◽  
Author(s):  
Steen Wilhelm Knudsen ◽  
Rasmus Bach Ebert ◽  
Martin Hesselsøe ◽  
Franziska Kuntke ◽  
Jakob Hassingboe ◽  
...  
Keyword(s):  
Baltic Sea ◽  
Environmental Dna ◽  
Marine Fishes ◽  
Specific Detection ◽  
The Baltic Sea ◽  
The Baltic ◽  
Species Specific ◽  
Detection And Quantification

Species-specific detection and quantification of common barnacle larvae from the Japanese coast using quantitative real-time PCR

Biofouling ◽  
2010 ◽  
Vol 26 (8) ◽  
pp. 901-911 ◽  
Author(s):  
Noriyuki Endo ◽  
Kana Sato ◽  
Kiyotaka Matsumura ◽  
Erina Yoshimura ◽  
Yukiko Odaka ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Specific Detection ◽  
Japanese Coast ◽  
Species Specific ◽  
Detection And Quantification

64: Rapid, Species-Specific Detection of Uropathogens from Clinical Urine Specimens Using an Electrochemical Microsensor Array

2005 ◽  
Vol 173 (4S) ◽  
pp. 18-18
Author(s):  
Joseph C. Liao ◽  
Mitra Mastali ◽  
David A. Haake ◽  
Bernard M. Churchill
Keyword(s):  
Specific Detection ◽  
Species Specific ◽  
Urine Specimens

scholarly journals A Forensic Detection Method for Hallucinogenic Mushrooms via High-Resolution Melting (HRM) Analysis

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 199
Author(s):  
Xiaochun Zhang ◽  
Huan Yu ◽  
Qi Yang ◽  
Ziwei Wang ◽  
Ruocheng Xia ◽  
...  
Keyword(s):  
High Resolution ◽  
Dna Barcoding ◽  
High Resolution Melting ◽  
Its Region ◽  
Its Sequencing ◽  
Melting Temperatures ◽  
Hrm Analysis ◽  
National Drug ◽  
Species Specific ◽  
Its1 And Its2

In recent years, trafficking and abuse of hallucinogenic mushrooms have become a serious social problem. It is therefore imperative to identify hallucinogenic mushrooms of the genus Psilocybe for national drug control legislation. An internal transcribed spacer (ITS) is a DNA barcoding tool utilized for species identification. Many methods have been used to discriminate the ITS region, but they are often limited by having a low resolution. In this study, we sought to analyze the ITS and its fragments, ITS1 and ITS2, by using high-resolution melting (HRM) analysis, which is a rapid and sensitive method for evaluating sequence variation within PCR amplicons. The ITS HRM assay was tested for specificity, reproducibility, sensitivity, and the capacity to analyze mixture samples. It was shown that the melting temperatures of the ITS, ITS1, and ITS2 of Psilocybe cubensis were 83.72 ± 0.01, 80.98 ± 0.06, and 83.46 ± 0.08 °C, and for other species, we also obtained species-specific results. Finally, we performed ITS sequencing to validate the presumptive taxonomic identity of our samples, and the sequencing output significantly supported our HRM data. Taken together, these results indicate that the HRM method can quickly distinguish the DNA barcoding of Psilocybe cubensis and other fungi, which can be utilized for drug trafficking cases and forensic science.

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