scholarly journals State‐of‐the‐art CRISPR /Cas9 Technology for Genome Editing in Trypanosomatids

2019 ◽  
Vol 66 (6) ◽  
pp. 981-991 ◽  
Author(s):  
Noelia Lander ◽  
Miguel A. Chiurillo
Keyword(s):  
Genome Editing ◽  
State Of The Art

Prime editing: The state-of-the-art of genome editing

Meta Gene ◽  
2020 ◽  
Vol 24 ◽  
pp. 100661
Author(s):  
Fatemeh Khatami ◽  
Maryam Aghaii ◽  
Seyed Mohammad Kazem Aghamir
Keyword(s):  
Genome Editing ◽  
State Of The Art ◽  
The State

scholarly journals Next-Generation Gene Therapy for Parkinson’s Disease Using Engineered Viral Vectors

2021 ◽  
pp. 1-9
Author(s):  
Tomas Björklund ◽  
Marcus Davidsson
Keyword(s):  
Parkinson’S Disease ◽  
Gene Therapy ◽  
Parkinson's Disease ◽  
Clinical Trials ◽  
Genome Editing ◽  
Viral Vectors ◽  
State Of The Art ◽  
The State ◽  
Next Generation ◽  
Suitable Vector

Recent technological and conceptual advances have resulted in a plethora of exciting novel engineered adeno associated viral (AAV) vector variants. They all have unique characteristics and abilities. This review summarizes the development and their potential in treating Parkinson’s disease (PD). Clinical trials in PD have shown over the last decade that AAV is a safe and suitable vector for gene therapy but that it also is a vehicle that can benefit significantly from improvement in specificity and potency. This review provides a concise collection of the state-of-the-art for synthetic capsids and their utility in PD. We also summarize what therapeutical strategies may become feasible with novel engineered vectors, including genome editing and neuronal rejuvenation.

scholarly journals CRISPR/Cas Technologies and Their Applications in Escherichia coli

2021 ◽  
Vol 9 ◽  
Author(s):  
Huina Dong ◽  
Yali Cui ◽  
Dawei Zhang
Keyword(s):  
Escherichia Coli ◽  
Transcriptional Regulation ◽  
Homologous Recombination ◽  
Genome Editing ◽  
State Of The Art ◽  
Bacterial Species ◽  
The State ◽  
Research Progress ◽  
End Joining ◽  
Eukaryotic Organism

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. However, these systems unfortunately have not been developed and applied in bacteria as extensively as in eukaryotic organism. Here, the research progress on the most widely used CRISPR/Cas tools and their applications in Escherichia coli is summarized. Genome editing based on homologous recombination, non-homologous DNA end-joining, transposons, and base editors are discussed. Finally, the state of the art of transcriptional regulation using CRISPRi is briefly reviewed. This review provides a useful reference for the application of CRISPR/Cas systems in other bacterial species.

CRISPR/Cas9-based genome editing of the filamentous fungi: the state of the art

2017 ◽  
Vol 101 (20) ◽  
pp. 7435-7443 ◽  
Author(s):  
Tian-Qiong Shi ◽  
Guan-Nan Liu ◽  
Rong-Yu Ji ◽  
Kun Shi ◽  
Ping Song ◽  
...  
Keyword(s):  
Genome Editing ◽  
Filamentous Fungi ◽  
State Of The Art ◽  
The State

CRISPR/Cas-based genome engineering in natural product discovery

2019 ◽  
Vol 36 (9) ◽  
pp. 1262-1280 ◽  
Author(s):  
Yaojun Tong ◽  
Tilmann Weber ◽  
Sang Yup Lee
Keyword(s):  
Natural Product ◽  
Genome Editing ◽  
Genome Engineering ◽  
State Of The Art ◽  
Natural Product Discovery ◽  
Current State

This review summarizes the current state of the art of CRISPR/Cas-based genome editing technologies for natural product producers.

scholarly journals How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing

2018 ◽  
Vol 46 (13) ◽  
pp. 6435-6454 ◽  
Author(s):  
Yannik Bollen ◽  
Jasmin Post ◽  
Bon-Kyoung Koo ◽  
Hugo J G Snippert
Keyword(s):  
Genome Editing ◽  
Genetic Model ◽  
State Of The Art ◽  
Model Systems

Genome Editing: State of the Art and Application to Animal Husbandry

2018 ◽  
Vol 34 (3) ◽  
pp. 9-22
Author(s):  
N.A. Zinovieva ◽  
◽  
N.A. Volkova ◽  
V.A. Bagirov
Keyword(s):  
Genome Editing ◽  
State Of The Art ◽  
Animal Husbandry
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