Molecular Features and mRNA Expression of the Receptor for Activated C Kinase 1 from Symbiodinium microadriaticum ssp. microadriaticum During Growth and the Light/Dark cycle

2018 ◽  
Vol 66 (2) ◽  
pp. 254-266
Author(s):  
Tania Islas-Flores ◽  
Esmeralda Pérez-Cervantes ◽  
Jessica Nava-Galeana ◽  
Montserrat Loredo-Guillén ◽  
Gabriel Guillén ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Dark Cycle ◽  
Symbiodinium Microadriaticum ◽  
Molecular Features

scholarly journals MicroRNA profiles of t(14;18)–negative follicular lymphoma support a late germinal center B-cell phenotype

Blood ◽  
2011 ◽  
Vol 118 (20) ◽  
pp. 5550-5558 ◽  
Author(s):  
Ellen Leich ◽  
Alberto Zamo ◽  
Heike Horn ◽  
Eugenia Haralambieva ◽  
Bernhard Puppe ◽  
...  
Keyword(s):  
B Cell ◽  
Mrna Expression ◽  
Germinal Center ◽  
Target Genes ◽  
Expression Patterns ◽  
Cell Phenotype ◽  
B Cell Differentiation ◽  
Molecular Features ◽  
Germinal Center B Cell ◽  
Bcl2 Expression

Abstract A total of 90% of follicular lymphomas (FLs) harbor the translocation t(14;18) leading to deregulated BCL2 expression. Conversely, 10% of FLs lack the t(14;18), and the majority of these FLs do not express BCL2. The molecular features of t(14;18)–negative FLs remain largely unknown. We performed microRNA expression analysis in 32 FL grades 1 to 3A, including 17 t(14;18)–positive FLs, 9 t(14;18)–negative FLs without BCL2 expression, and 6 t(14;18)–negative FLs with BCL2 expression. MicroRNA profiles were correlated with corresponding mRNA expression patterns, and potential targets were investigated by quantitative PCR and immunohistochemistry in an independent validation series of 83 FLs. Statistical analysis identified 17 microRNAs that were differentially expressed between t(14;18)–positive FLs and t(14;18)–negative FLs. The down-regulation of miR-16, miR-26a, miR-101, miR-29c, and miR138 in the t(14;18)-negative FL subset was associated with profound mRNA expression changes of potential target genes involving cell cycle control, apoptosis, and B-cell differentiation. miR-16 target CHEK1 showed increased expression in t(14;18)-negative FLs, whereas TCL1A expression was reduced, in line with a partial loss of the germinal center B-cell phenotype in this FL subset. In conclusion, t(14;18)–negative FL have distinct microRNA profiles that are associated with an increased proliferative capacity and a “late” germinal center B-cell phenotype.

Dystrophin deficiency disrupts muscle clock expression and mitochondrial quality control in mdx mice

2021 ◽  
Author(s):  
Justin P. Hardee ◽  
Marissa K. Caldow ◽  
Audrey S.M. Chan ◽  
Stuart K. Plenderleith ◽  
Jennifer Trieu ◽  
...  
Keyword(s):  
Quality Control ◽  
Mrna Expression ◽  
Tibialis Anterior ◽  
Oxidative Capacity ◽  
Mdx Mice ◽  
Dark Cycle ◽  
Mitochondrial Quality Control ◽  
The Core ◽  
Dystrophin Deficiency ◽  
Diurnal Regulation

Impaired oxidative capacity and mitochondrial function contribute to the dystrophic pathology in muscles of Duchenne muscular dystrophy (DMD) patients and in relevant mouse models of the disease. Emerging evidence suggests an association between disrupted core clock expression and mitochondrial quality control, but this has not been established in muscles lacking dystrophin. We examined the diurnal regulation of muscle core clock and mitochondrial quality control expression in dystrophin-deficient C57BL/10ScSn-Dmdmdx (mdx) mice, an established model of DMD. Male C57BL/10 (BL/10; n=18) and mdx mice (n=18) were examined every 4 hours beginning at the dark cycle. Throughout the entire light-dark cycle, extensor digitorum longus (EDL) muscles from mdx mice had decreased core clock mRNA expression (Arntl, Cry1, Cry2, Nr1d2; p<0.05) and disrupted mitochondrial quality control mRNA expression related to biogenesis (decreased; Ppargc1a, Esrra; p<0.05), fission (increased; Dnm1l; p<0.01), fusion (decreased; Opa1, Mfn1; p<0.05) and autophagy/mitophagy (decreased: Bnip3; p<0.05; increased: Becn1; p<0.05). Cosinor analysis revealed a decrease in the rhythmicity parameters mesor and amplitude for Arntl, Cry1, Cry2, Per2, and Nr1d1 (p<0.001) in mdx mice. Diurnal oscillations in Esrra, Sirt1, Map1lc3b and Sqstm1 were absent in mdx mice, along with decreased mesor and amplitude of Ppargc1a mRNA expression (p<0.01). The expression of proteins involved in mitochondrial biogenesis (decreased: PPARGC1A, p<0.05) and autophagy/mitophagy (increased: MAP1LC3BII, SQSTM1, BNIP3; p<0.05) were also dysregulated in tibialis anterior muscles of mdx mice. These findings suggest that dystrophin deficiency in mdx mice impairs the regulation of the core clock and mitochondrial quality control, with relevance to DMD and related disorders.

scholarly journals Integrating microRNA and mRNA expression profiling in Symbiodinium microadriaticum, a dinoflagellate symbiont of reef-building corals

BMC Genomics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 704 ◽  
Author(s):  
Sebastian Baumgarten ◽  
Till Bayer ◽  
Manuel Aranda ◽  
Yi Liew ◽  
Adrian Carr ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Expression Profiling ◽  
Symbiodinium Microadriaticum ◽  
Mrna Expression Profiling

scholarly journals Desipramine restores the alterations in circadian entrainment induced by prenatal exposure to glucocorticoids

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Stefan Spulber ◽  
Mirko Conti ◽  
Frederik Elberling ◽  
Marilena Raciti ◽  
Dasiel Oscar Borroto-Escuela ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Feedback Loop ◽  
Nuclear Translocation ◽  
Clock Genes ◽  
Negative Feedback Loop ◽  
Dark Cycle ◽  
Peripheral Oscillators ◽  
Noradrenaline Reuptake ◽  
Peripheral Clocks ◽  
Central Clock

Abstract Alterations in circadian rhythms are closely linked to depression, and we have shown earlier that progressive alterations in circadian entrainment precede the onset of depression in mice exposed in utero to excess glucocorticoids. The aim of this study was to investigate whether treatment with the noradrenaline reuptake inhibitor desipramine (DMI) could restore the alterations in circadian entrainment and prevent the onset of depression-like behavior. C57Bl/6 mice were exposed to dexamethasone (DEX—synthetic glucocorticoid analog, 0.05 mg/kg/day) between gestational day 14 and delivery. Male offspring aged 6 months (mo) were treated with DMI (10 mg/kg/day in drinking water) for at least 21 days before behavioral testing. We recorded spontaneous activity using the TraffiCage™ system and found that DEX mice re-entrained faster than controls after an abrupt advance in light-dark cycle by 6 h, while DMI treatment significantly delayed re-entrainment. Next we assessed the synchronization of peripheral oscillators with the central clock (located in the suprachiasmatic nucleus—SCN), as well as the mechanisms required for entrainment. We found that photic entrainment of the SCN was apparently preserved in DEX mice, but the expression of clock genes in the hippocampus was not synchronized with the light-dark cycle. This was associated with downregulated mRNA expression for arginine vasopressin (AVP; the main molecular output entraining peripheral clocks) in the SCN, and for glucocorticoid receptor (GR; required for the negative feedback loop regulating glucocorticoid secretion) in the hippocampus. DMI treatment restored the mRNA expression of AVP in the SCN and enhanced GR-mediated signaling by upregulating GR expression and nuclear translocation in the hippocampus. Furthermore, DMI treatment at 6 mo prevented the onset of depression-like behavior and the associated alterations in neurogenesis in 12-mo-old DEX mice. Taken together, our data indicate that DMI treatment enhances GR-mediated signaling and restores the synchronization of peripheral clocks with the SCN and support the hypothesis that altered circadian entrainment is a modifiable risk factor for depression.

scholarly journals Differing total mRNA expression shapes the molecular and clinical phenotype of cancer

2020 ◽  
Author(s):  
Shaolong Cao ◽  
Jennifer R. Wang ◽  
Shuangxi Ji ◽  
Peng Yang ◽  
Jingxiao Chen ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Single Cell ◽  
Computational Method ◽  
Model Systems ◽  
Intratumor Heterogeneity ◽  
Tumor Type ◽  
Single Cell Sequencing ◽  
Molecular Features ◽  
Increased Risk ◽  
Mrna Content

AbstractCancers can vary greatly in their transcriptomes. In contrast to alterations in specific genes or pathways, the significance of differences in tumor cell total mRNA content is poorly understood. Studies using single-cell sequencing or model systems have suggested a role for total mRNA content in regulating cellular phenotypes. However, analytical challenges related to technical artifacts and cellular admixture have impeded examination of total mRNA expression at scale across cancers. To address this, we evaluated total mRNA expression using single cell sequencing, and developed a computational method for quantifying tumor-specific total mRNA expression (TmS) from bulk sequencing data. We systematically estimated TmS in 5,181 patients across 15 cancer types and observed close correlations with clinicopathologic characteristics and molecular features, where high TmS generally accompanies high-risk disease. At a pan-cancer level, high TmS is associated with increased risk of disease progression and death. Moreover, TmS captures tumor type-specific effects of somatic mutations, chromosomal instability, and hypoxia, as well as aspects of intratumor heterogeneity. Taken together, our results suggest that measuring total mRNA expression offers a broader perspective of tracking cancer transcriptomes, which has important clinical and biological implications.

scholarly journals Compensation for cold-induced thermogenesis during weight loss maintenance and regain

2019 ◽  
Vol 316 (5) ◽  
pp. E977-E986 ◽  
Author(s):  
David M. Presby ◽  
Matthew R. Jackman ◽  
Michael C. Rudolph ◽  
Vanessa D. Sherk ◽  
Rebecca M. Foright ◽  
...  
Keyword(s):  
Weight Loss ◽  
Mrna Expression ◽  
Cold Exposure ◽  
Weight Loss Maintenance ◽  
Light Cycle ◽  
Dark Cycle ◽  
Early Relapse ◽  
Compensatory Behaviors ◽  
Brown Adipose ◽  
Prevalence Of Obesity

Prevalence of obesity is exacerbated by low rates of successful long-term weight loss maintenance (WLM). In part, relapse from WLM to obesity is due to a reduction in energy expenditure (EE) that persists throughout WLM and relapse. Thus, interventions that increase EE might facilitate WLM. In obese mice that were calorically restricted to reduce body weight by ~20%, we manipulated EE throughout WLM and early relapse using intermittent cold exposure (ICE; 4°C, 90 min/day, 5 days/wk, within the last 3 h of the light cycle). EE, energy intake, and spontaneous physical activity were measured during the obese, WLM, and relapse phases. During WLM and relapse, the ICE group expended more energy during the light cycle because of cold exposure but expended less energy in the dark cycle, which led to no overall difference in total daily EE. The compensation in EE appeared to be mediated by activity, whereby the ICE group was more active during the light cycle because of cold exposure but less active during the dark cycle, which led to no overall effect on total daily activity during WLM and relapse. In brown adipose tissue of relapsing mice, the ICE group had greater mRNA expression of Dio2 and protein expression of UCP1 but lower mRNA expression of Prdm16. In summary, these findings indicate that despite robust increases in EE during cold exposures, ICE is unable to alter total daily EE during WLM or early relapse, likely due to compensatory behaviors in activity.

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