The Nuclear RNA-binding Protein RBSR1 Interactome in Trypanosoma cruzi

2018 ◽  
Vol 66 (2) ◽  
pp. 244-253 ◽  
Author(s):  
Helisa H. Wippel ◽  
Juliane S. Malgarin ◽  
Sharon de Toledo Martins ◽  
Newton M. Vidal ◽  
Bruna H. Marcon ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Nuclear Rna

scholarly journals Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis.

1995 ◽  
Vol 182 (3) ◽  
pp. 865-874 ◽  
Author(s):  
Q Tian ◽  
J Taupin ◽  
S Elledge ◽  
M Robertson ◽  
P Anderson
Keyword(s):  
Dna Fragmentation ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Jurkat Cells ◽  
Serine Threonine Kinase ◽  
Threonine Kinase ◽  
Nuclear Rna

We have identified a serine/threonine kinase that is rapidly activated during Fas-mediated apoptosis. Fas-activated serine/threonine kinase (FAST) is phosphorylated on serine and threonine residues in Jurkat cells. In response to Fas ligation, it is rapidly dephosphorylated and concomitantly activated to phosphorylate TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Phosphorylation of TIA-1 precedes the onset of DNA fragmentation, suggesting a role in signaling downstream events in the apoptotic program. Our results introduce Fast and TIA-1 as components of a molecular cascade involved in signaling Fas-mediated apoptosis.

scholarly journals The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.

1997 ◽  
Vol 17 (6) ◽  
pp. 3194-3201 ◽  
Author(s):  
R J Buckanovich ◽  
R B Darnell
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Motor Dysfunction ◽  
High Affinity ◽  
Loop Region ◽  
Nuclear Rna ◽  
Rna Ligands

Nova-1, an autoantigen in paraneoplastic opsoclonus myoclonus ataxia (POMA), a disorder associated with breast cancer and motor dysfunction, is a neuron-specific nuclear RNA binding protein. We have identified in vivo Nova-1 RNA ligands by combining affinity-elution-based RNA selection with protein-RNA immunoprecipitation. Starting with a pool of approximately 10(15) random 52-mer RNAs, we identified long stem-loop RNA ligands that bind to Nova-1 with high affinity (Kd of approximately 2 nM). The loop region of these RNAs harbors a approximately 15-bp pyrimidine-rich element [UCAU(N)(0-2)]3 which is essential for Nova-1 binding. Mutagenesis studies defined the third KH domain of Nova-1 and the [UCAU(N)(0-2)]3 element as necessary for in vitro binding. Consensus [UCAU (N)(0-2)], elements were identified in two neuronal pre-mRNAs, one encoding the inhibitory glycine receptor alpha2 (GlyR alpha2) and a second encoding Nova-1 itself. Nova-1 protein binds these RNAs with high affinity and specificity in vitro, and this binding can be blocked by POMA antisera. Moreover, both Nova-1 and GlyR alpha2 pre-mRNAs specifically coimmunoprecipitated with Nova-1 protein from brain extracts. Thus, Nova-1 functions as a sequence-specific nuclear RNA binding protein in vivo; disruption of the specific interaction between Nova-1 and GlyR alpha2 pre-mRNA may underlie the motor dysfunction seen in POMA.

Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family

2006 ◽  
Vol 113 (2) ◽  
pp. 112-124 ◽  
Author(s):  
Florence Caro ◽  
Natalia Bercovich ◽  
Catalina Atorrasagasti ◽  
Mariano J. Levin ◽  
Martín P. Vázquez
Keyword(s):  
Trypanosoma Cruzi ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Protein Family

scholarly journals The nuclear RNA binding protein RBP33 influences mRNA and spliced leader RNA abundance in Trypanosoma brucei

2017 ◽  
Vol 212 ◽  
pp. 16-20 ◽  
Author(s):  
Olivera Cirovic ◽  
Roman Trikin ◽  
Anneliese Hoffmann ◽  
Nicholas Doiron ◽  
Martin Jakob ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Spliced Leader ◽  
Nuclear Rna ◽  
Spliced Leader Rna

scholarly journals The overexpression of the trypanosomatid-exclusive TcRBP19 RNA-binding protein affects cellular infection by Trypanosoma cruzi

2012 ◽  
Vol 107 (8) ◽  
pp. 1076-1079 ◽  
Author(s):  
Leticia Pérez-Díaz ◽  
Alejandro Correa ◽  
Mariana P Moretão ◽  
Samuel Goldenberg ◽  
Bruno Dallagiovanna ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein

scholarly journals Haploinsufficiency of the germ cell-specific nuclear RNA binding protein hnRNP G-T prevents functional spermatogenesis in the mouse

2008 ◽  
Vol 17 (18) ◽  
pp. 2803-2818 ◽  
Author(s):  
Ingrid Ehrmann ◽  
Caroline Dalgliesh ◽  
Aikaterini Tsaousi ◽  
Maria Paola Paronetto ◽  
Bettina Heinrich ◽  
...  
Keyword(s):  
Germ Cell ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Nuclear Rna

scholarly journals Molecular characterization of the Trypanosoma cruzi specific RNA binding protein TcRBP40 and its associated mRNAs

2012 ◽  
Vol 420 (2) ◽  
pp. 302-307 ◽  
Author(s):  
Eloise P. Guerra-Slompo ◽  
Christian M. Probst ◽  
Daniela P. Pavoni ◽  
Samuel Goldenberg ◽  
Marco A. Krieger ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Molecular Characterization ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein
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