Nuclear Features of the Heterotrich CiliateBlepharisma americanum: Genomic Amplification, Life Cycle, and Nuclear Inclusion

Megan M. Wancura; Ying Yan; Laura A. Katz; Xyrus X. Maurer-Alcalá

doi:10.1111/jeu.12422

Nuclear Features of the Heterotrich CiliateBlepharisma americanum: Genomic Amplification, Life Cycle, and Nuclear Inclusion

Journal of Eukaryotic Microbiology ◽

10.1111/jeu.12422 ◽

2017 ◽

Vol 65 (1) ◽

pp. 4-11 ◽

Cited By ~ 5

Author(s):

Megan M. Wancura ◽

Ying Yan ◽

Laura A. Katz ◽

Xyrus X. Maurer-Alcalá

Keyword(s):

Life Cycle ◽

Nuclear Inclusion ◽

Genomic Amplification ◽

Nuclear Features

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Origin of Hepatic Nuclear Inclusion Bodies

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072575 ◽

1973 ◽

Vol 31 ◽

pp. 426-427

Author(s):

F. G. Zaki ◽

J. A. Greenlee ◽

C. H. Keysser

Keyword(s):

Inclusion Bodies ◽

Storage Disease ◽

Glycogen Storage ◽

Nutritional Deficiencies ◽

Nuclear Inclusion ◽

Electron Microscopic ◽

Human Liver Cells ◽

Microscopic Studies ◽

Per Se ◽

Experimental Liver

Nuclear inclusion bodies seen in human liver cells may appear in light microscopy as deposits of fat or glycogen resulting from various diseases such as diabetes, hepatitis, cholestasis or glycogen storage disease. These deposits have been also encountered in experimental liver injury and in our animals subjected to nutritional deficiencies, drug intoxication and hepatocarcinogens. Sometimes these deposits fail to demonstrate the presence of fat or glycogen and show PAS negative reaction. Such deposits are considered as viral products.Electron microscopic studies of these nuclei revealed that such inclusion bodies were not products of the nucleus per se but were mere segments of endoplasmic reticulum trapped inside invaginating nuclei (Fig. 1-3).

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Cytoplasmic Invaginations in Trophoblasts and Epithelial Cells of Rat

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006667x ◽

1971 ◽

Vol 29 ◽

pp. 524-525

Author(s):

Iracema M. Baccarini

Keyword(s):

Osmium Tetroxide ◽

Uranyl Acetate ◽

Nuclear Inclusions ◽

Mucous Cells ◽

Electron Microscopic ◽

Cervical Epithelium ◽

Intact Membrane ◽

Microscopic Studies ◽

Abnormal Cells ◽

Nuclear Features

Some morphological nuclear features (invaginations) in normal and abnormal cells have been described in several electron microscopic studies. They have been referred to by others as blebs, loops, pockets, sheets, bodies, nuclear inclusions and cytoplasmic invaginations. Identical appearing structures were found in cells of the uterine cervical epithelium, in trophoblasts of blastocysts and in trophoblasts of rat placenta.Methods. Uterine cervix (normal rats), rat placenta (9-10 days gestation) and blastocyst were placed in 3% glutarahdehyde for 3 hours. The tissue was washed in phosphate buffer for 24 hours, postfixed in 1%. buffered osmium tetroxide for 1-2 hours and embedded in epon araldite. Sections were double stained with uranyl acetate and lead citrate and viewed in E. M. Siemens 200.Observations. Nuclear invaginations were found in basal, parabasal and mucous cells of the cervix epithelium, in trophoblasts of blastocyst and in trophoblasts of placenta. An oval, round or elongated invagination contained heterogenously cytoplasm surrounded by a double intact membrane; usually several invaginations were found in the same nucleus.

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Ultrastructural analysis of “Sensory” surface nerve endings and “putative” neurotransmitter sites in Schistosoma mansoni Miracidia

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156791 ◽

1989 ◽

Vol 47 ◽

pp. 962-963

Author(s):

Betty Ruth Jones ◽

Steve Chi-Tang Pan

Keyword(s):

Nervous System ◽

Life Cycle ◽

Schistosoma Mansoni ◽

Snail Host ◽

Complex Life Cycle ◽

Rational Approach ◽

Effective Control ◽

The Social ◽

Social And Economic Development ◽

Basic Biology

INTRODUCTION: Schistosomiasis has been described as “one of the most devastating diseases of mankind, second only to malaria in its deleterious effects on the social and economic development of populations in many warm areas of the world.” The disease is worldwide and is probably spreading faster and becoming more intense than the overall research efforts designed to provide the basis for countering it. Moreover, there are indications that the development of water resources and the demands for increasing cultivation and food in developing countries may prevent adequate control of the disease and thus the number of infections are increasing.Our knowledge of the basic biology of the parasites causing the disease is far from adequate. Such knowledge is essential if we are to develop a rational approach to the effective control of human schistosomiasis. The miracidium is the first infective stage in the complex life cycle of schistosomes. The future of the entire life cycle depends on the capacity and ability of this organism to locate and enter a suitable snail host for further development, Little is known about the nervous system of the miracidium of Schistosoma mansoni and of other trematodes. Studies indicate that miracidia contain a well developed and complex nervous system that may aid the larvae in locating and entering a susceptible snail host (Wilson, 1970; Brooker, 1972; Chernin, 1974; Pan, 1980; Mehlhorn, 1988; and Jones, 1987-1988).

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A freeze-fracture-etched study of the physarum polycephalum plasma membrane during early formation of the macroplasmodial stage

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147570 ◽

1993 ◽

Vol 51 ◽

pp. 346-347

Author(s):

Randolph W. Taylor ◽

Henrie Treadwell

Keyword(s):

Plasma Membrane ◽

Life Cycle ◽

Growth Phase ◽

Filter Paper ◽

Physarum Polycephalum ◽

Freeze Fracture ◽

Petri Dish ◽

Wire Screen ◽

Wide Mouth ◽

Sterile Filter

The plasma membrane of the Slime Mold, Physarum polycephalum, process unique morphological distinctions at different stages of the life cycle. Investigations of the plasma membrane of P. polycephalum, particularly, the arrangements of the intramembranous particles has provided useful information concerning possible changes occurring in higher organisms. In this report Freeze-fracture-etched techniques were used to investigate 3 hours post-fusion of the macroplasmodia stage of the P. polycephalum plasma membrane.Microplasmodia of Physarum polycephalum (M3C), axenically maintained, were collected in mid-expotential growth phase by centrifugation. Aliquots of microplasmodia were spread in 3 cm circles with a wide mouth pipette onto sterile filter paper which was supported on a wire screen contained in a petri dish. The cells were starved for 2 hrs at 24°C. After starvation, the cells were feed semidefined medium supplemented with hemin and incubated at 24°C. Three hours after incubation, samples were collected randomly from the petri plates, placed in plancettes and frozen with a propane-nitrogen jet freezer.

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