Characterization of the First Fungal Glycosyl Hydrolase Family 19 Chitinase (NbchiA) from Nosema bombycis (Nb)

2015 ◽  
Vol 63 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Bing Han ◽  
Kang Zhou ◽  
Zhihong Li ◽  
Bin Sun ◽  
Qi Ni ◽  
...  
Keyword(s):  
Glycosyl Hydrolase ◽  
Glycosyl Hydrolase Family ◽  
Nosema Bombycis ◽  
Family 19 Chitinase ◽  
Hydrolase Family

scholarly journals Characterization of Two Novel α-Glucosidases from Bifidobacterium breve UCC2003

2008 ◽  
Vol 75 (4) ◽  
pp. 1135-1143 ◽  
Author(s):  
Karina Pokusaeva ◽  
Mary O'Connell-Motherway ◽  
Aldert Zomer ◽  
Gerald F. Fitzgerald ◽  
Douwe van Sinderen
Keyword(s):  
Glycosyl Hydrolase ◽  
Hydrolytic Activity ◽  
Glycosyl Hydrolase Family ◽  
Bifidobacterium Breve ◽  
Transglycosylation Activity ◽  
Encoding Genes ◽  
Temperature Optima ◽  
Hydrolase Family

ABSTRACT Two α-glucosidase-encoding genes (agl1 and agl2) from Bifidobacterium breve UCC2003 were identified and characterized. Based on their similarity to characterized carbohydrate hydrolases, the Agl1 and Agl2 enzymes are both assigned to a subgroup of the glycosyl hydrolase family 13, the α-1,6-glucosidases (EC 3.2.1.10). Recombinant Agl1 and Agl2 into which a His12 sequence was incorporated (Agl1His and Agl2His, respectively) exhibited hydrolytic activity towards panose, isomaltose, isomaltotriose, and four sucrose isomers—palatinose, trehalulose, turanose, and maltulose—while also degrading trehalose and, to a lesser extent, nigerose. The preferred substrates for both enzymes were panose, isomaltose, and trehalulose. Furthermore, the pH and temperature optima for both enzymes were determined, showing that Agl1His exhibits higher thermo and pH optima than Agl2His. The two purified α-1,6-glucosidases were also shown to have transglycosylation activity, synthesizing oligosaccharides from palatinose, trehalulose, trehalose, panose, and isomaltotriose.

scholarly journals Specific Characterization of Substrate and Inhibitor Binding Sites of a Glycosyl Hydrolase Family 11 Xylanase fromAspergillus niger

2002 ◽  
Vol 277 (46) ◽  
pp. 44035-44043 ◽  
Author(s):  
Tariq A. Tahir ◽  
Jean-Guy Berrin ◽  
Ruth Flatman ◽  
Alain Roussel ◽  
Peter Roepstorff ◽  
...  
Keyword(s):  
Binding Sites ◽  
Glycosyl Hydrolase ◽  
Glycosyl Hydrolase Family ◽  
Inhibitor Binding ◽  
Hydrolase Family
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