Comparing High-throughput Platforms for Sequencing the V4 Region of SSU-rDNA in Environmental Microbial Eukaryotic Diversity Surveys

2014 ◽  
Vol 62 (3) ◽  
pp. 338-345 ◽  
Author(s):  
Frédéric Mahé ◽  
Jordan Mayor ◽  
John Bunge ◽  
Jingyun Chi ◽  
Tobias Siemensmeyer ◽  
...  
Keyword(s):  
High Throughput ◽  
Ssu Rdna ◽  
Eukaryotic Diversity

scholarly journals Assessing the responses of Sphagnum micro-eukaryotes to climate changes using high throughput sequencing

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9821 ◽  
Author(s):  
Monika K. Reczuga ◽  
Christophe Victor William Seppey ◽  
Matthieu Mulot ◽  
Vincent E.J. Jassey ◽  
Alexandre Buttler ◽  
...  
Keyword(s):  
High Throughput ◽  
Ecosystem Functioning ◽  
High Throughput Sequencing ◽  
Ecosystem Respiration ◽  
Carbon Sources ◽  
Environmental Dna ◽  
Severe Drought ◽  
Food Web Interactions ◽  
Eukaryotic Diversity ◽  
Eukaryotic Microorganisms

Current projections suggest that climate warming will be accompanied by more frequent and severe drought events. Peatlands store ca. one third of the world’s soil organic carbon. Warming and drought may cause peatlands to become carbon sources through stimulation of microbial activity increasing ecosystem respiration, with positive feedback effect on global warming. Micro-eukaryotes play a key role in the carbon cycle through food web interactions and therefore, alterations in their community structure and diversity may affect ecosystem functioning and could reflect these changes. We assessed the diversity and community composition of Sphagnum-associated eukaryotic microorganisms inhabiting peatlands and their response to experimental drought and warming using high throughput sequencing of environmental DNA. Under drier conditions, micro-eukaryotic diversity decreased, the relative abundance of autotrophs increased and that of osmotrophs (including Fungi and Peronosporomycetes) decreased. Furthermore, we identified climate change indicators that could be used as early indicators of change in peatland microbial communities and ecosystem functioning. The changes we observed indicate a shift towards a more “terrestrial” community in response to drought, in line with observed changes in the functioning of the ecosystem.

scholarly journals High-throughput metabarcoding of eukaryotic diversity for environmental monitoring of offshore oil-drilling activities

2016 ◽  
Vol 25 (17) ◽  
pp. 4392-4406 ◽  
Author(s):  
Anders Lanzén ◽  
Katrine Lekang ◽  
Inge Jonassen ◽  
Eric M. Thompson ◽  
Christofer Troedsson
Keyword(s):  
Environmental Monitoring ◽  
High Throughput ◽  
Oil Drilling ◽  
Eukaryotic Diversity ◽  
Offshore Oil

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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