Lethal RNA interference response in the pepper weevil

2019 ◽  
Vol 143 (7) ◽  
pp. 699-705 ◽  
Author(s):  
Ke Wu ◽  
Caitlin E. Taylor ◽  
Daniele H. Pinheiro ◽  
Lucile H. Skelley ◽  
Heather J. McAuslane ◽  
...  
Keyword(s):  
Rna Interference ◽  
Pepper Weevil ◽  
Interference Response

An intact RNA interference pathway is required for expression of the mutant wing phenotype of vg21-3, a P-element-induced allele of the vestigial gene in Drosophila

Genome ◽  
2012 ◽  
Vol 55 (4) ◽  
pp. 312-326 ◽  
Author(s):  
Ross B. Hodgetts ◽  
Sandra L. O’Keefe ◽  
Kyle J. Anderson
Keyword(s):  
Rna Interference ◽  
Dna Sequencing ◽  
Mutant Phenotype ◽  
P Element ◽  
Type I ◽  
P Elements ◽  
Gene Encode ◽  
Interference Response ◽  
Partial Reversion

We have determined that two P elements, P[21-3] and P[21r36], residing in the 5′-UTR of the vestigial wing gene, encode functional repressors in eye tissue. However, neither element fits a previous categorization of repressor-making elements as Type I or II. Both elements encode polypeptides that are shorter than the canonical elements they most closely resemble. DNA sequencing reveals that P[21r36] encodes an intact THAP domain that is missing in the P[21] element, which does not encode a functional repressor. Recovery of P[21-3] at sites other than vestigial (where it causes the wing mutant, vg21-3) reveals that the element can make repressor in wing tissue of sufficient activity to repress the mutant phenotype of vg21-3. Why the P[21-3] element fails to produce repressor when located at vestigial may be explained by our observation that three different mutants in the RNA interference pathway cause a partial reversion of vg21-3. We speculate that the vg and P-initiated transcripts that arise at the vg locus in the vg21-3 mutant trigger an RNA interference response that results in the mutual degradation of both transcripts.

Vacuolar (H + )‐ ATPase subunit c is essential for the survival and systemic RNA interference response in Locusta migratoria

2022 ◽  
Author(s):  
Xuekai Shi ◽  
Xiaojian Liu ◽  
Anastasia M.W. Cooper ◽  
Kristopher Silver ◽  
Hans Merzendorfer ◽  
...  
Keyword(s):  
Rna Interference ◽  
Locusta Migratoria ◽  
Atpase Subunit ◽  
Subunit C ◽  
Systemic Rna ◽  
Interference Response

scholarly journals Cell-to-Cell Spread of the RNA Interference Response Suppresses Semliki Forest Virus (SFV) Infection of Mosquito Cell Cultures and Cannot Be Antagonized by SFV

2009 ◽  
Vol 83 (11) ◽  
pp. 5735-5748 ◽  
Author(s):  
Ghassem Attarzadeh-Yazdi ◽  
Rennos Fragkoudis ◽  
Yi Chi ◽  
Ricky W. C. Siu ◽  
Liane Ülper ◽  
...  
Keyword(s):  
Rna Interference ◽  
Semliki Forest Virus ◽  
Cultured Cells ◽  
Virus Production ◽  
Mosquito Cells ◽  
Infected Cells ◽  
Interference Response ◽  
Interfering Rna ◽  
Rnai Response ◽  
Trigger Cell Death

ABSTRACT In their vertebrate hosts, arboviruses such as Semliki Forest virus (SFV) (Togaviridae) generally counteract innate defenses and trigger cell death. In contrast, in mosquito cells, following an early phase of efficient virus production, a persistent infection with low levels of virus production is established. Whether arboviruses counteract RNA interference (RNAi), which provides an important antiviral defense system in mosquitoes, is an important question. Here we show that in Aedes albopictus-derived mosquito cells, SFV cannot prevent the establishment of an antiviral RNAi response or prevent the spread of protective antiviral double-stranded RNA/small interfering RNA (siRNA) from cell to cell, which can inhibit the replication of incoming virus. The expression of tombusvirus siRNA-binding protein p19 by SFV strongly enhanced virus spread between cultured cells rather than virus replication in initially infected cells. Our results indicate that the spread of the RNAi signal contributes to limiting virus dissemination.

scholarly journals C6/36 Aedes albopictus Cells Have a Dysfunctional Antiviral RNA Interference Response

2010 ◽  
Vol 4 (10) ◽  
pp. e856 ◽  
Author(s):  
Doug E. Brackney ◽  
Jaclyn C. Scott ◽  
Fumihiko Sagawa ◽  
Jimmy E. Woodward ◽  
Neil A. Miller ◽  
...  
Keyword(s):  
Rna Interference ◽  
Aedes Albopictus ◽  
Aedes Albopictus Cells ◽  
Interference Response

scholarly journals The Antiviral RNA Interference Response Provides Resistance to Lethal Arbovirus Infection and Vertical Transmission in Caenorhabditis elegans

2017 ◽  
Vol 27 (6) ◽  
pp. 795-806 ◽  
Author(s):  
Don B. Gammon ◽  
Takao Ishidate ◽  
Lichao Li ◽  
Weifeng Gu ◽  
Neal Silverman ◽  
...  
Keyword(s):  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Vertical Transmission ◽  
Arbovirus Infection ◽  
Interference Response

scholarly journals Escherichia coli Metabolite Profiling Leads to the Development of an RNA Interference Strain for Caenorhabditis elegans

2019 ◽  
Vol 10 (1) ◽  
pp. 189-198 ◽  
Author(s):  
Isaiah A. A. Neve ◽  
Jessica N. Sowa ◽  
Chih-Chun J. Lin ◽  
Priya Sivaramakrishnan ◽  
Christophe Herman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Mitochondrial Morphology ◽  
Bacterial Strains ◽  
Hybrid Strain ◽  
Fat Storage ◽  
C Elegans ◽  
Interference Response ◽  
K 12

The relationship of genotypes to phenotypes can be modified by environmental inputs. Such crucial environmental inputs include metabolic cues derived from microbes living together with animals. Thus, the analysis of genetic effects on animals’ physiology can be confounded by variations in the metabolic profile of microbes. Caenorhabditis elegans exposed to distinct bacterial strains and species exhibit phenotypes different at cellular, developmental, and behavioral levels. Here we reported metabolomic profiles of three Escherichia coli strains, B strain OP50, K-12 strain MG1655, and B-K-12 hybrid strain HB101, as well as different mitochondrial and fat storage phenotypes of C. elegans exposed to MG1655 and HB101 vs. OP50. We found that these metabolic phenotypes of C. elegans are not correlated with overall metabolic patterning of bacterial strains, but their specific metabolites. In particular, the fat storage phenotype is traced to the betaine level in different bacterial strains. HT115 is another K-12 E. coli strain that is commonly utilized to elicit an RNA interference response, and we showed that C. elegans exposed to OP50 and HT115 exhibit differences in mitochondrial morphology and fat storage levels. We thus generated an RNA interference competent OP50 (iOP50) strain that can robustly and consistently knockdown endogenous C. elegans genes in different tissues. Together, these studies suggest the importance of specific bacterial metabolites in regulating the host’s physiology and provide a tool to prevent confounding effects when analyzing genotype-phenotype interactions under different bacterial backgrounds.

scholarly journals Characterization of the RNA Interference Response in the Asian Citrus Psyllid

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Lindsay Shaffer ◽  
R. G. Shatters, Jr. ◽  
C. Powell ◽  
R. Cave ◽  
D. Borovsky
Keyword(s):  
Rna Interference ◽  
Asian Citrus Psyllid ◽  
Interference Response
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