Entomopathogenic nematodes as potential biological control agents of Popillia japonica (Coleoptera, Scarabaeidae) in Piedmont Region (Italy)

2017 ◽  
Vol 142 (3) ◽  
pp. 311-318 ◽  
Author(s):  
L. Marianelli ◽  
F. Paoli ◽  
G. Torrini ◽  
G. Mazza ◽  
C. Benvenuti ◽  
...  
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Popillia Japonica ◽  
Biological Control Agents ◽  
Piedmont Region

scholarly journals First Screening of Entomopathogenic Nematodes and Fungus as Biocontrol Agents against an Emerging Pest of Sugarcane, Cacosceles newmannii (Coleoptera: Cerambycidae)

Insects ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 117 ◽  
Author(s):  
Marion Javal ◽  
John S. Terblanche ◽  
Desmond E. Conlong ◽  
Antoinette P. Malan
Keyword(s):  
South Africa ◽  
Biological Control ◽  
Chemical Control ◽  
Entomopathogenic Nematodes ◽  
Biocontrol Agents ◽  
Sugar Production ◽  
Biological Control Agents ◽  
Sugarcane Stalk ◽  
Reduce Sugar ◽  
Steinernema Yirgalemense

Cacosceles newmannii (Coleoptera: Cerambycidae) is an emerging pest of sugarcane in South Africa. The larvae of this cerambycid beetle live within the sugarcane stalk and drill galleries that considerably reduce sugar production. To provide an alternative to chemical control, entomopathogenic nematodes and fungus were investigated as potential biological control agents to be used in an integrated pest management system. The nematodes Steinernema yirgalemense, S. jeffreyense, Heterorhabditis indica, and different concentrations of the fungus Metarhizium pinghaense were screened for efficacy (i.e., mortality rate) against larvae of C. newmannii. The different biocontrol agents used, revealed a low level of pathogenicity to C. newmannii larvae, when compared to control treatments.

Evaluation of Trap Cropping and Biological Control Against Southern Corn Rootworm (Coleopetera: Chrysomelidae) in Peanuts

1997 ◽  
Vol 32 (2) ◽  
pp. 229-243 ◽  
Author(s):  
M.E. Barbercheck ◽  
W.C. Warrick
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Field Trials ◽  
Corn Rootworm ◽  
Cucurbita Maxima ◽  
Biological Control Agents ◽  
Trap Crop ◽  
Diabrotica Undecimpunctata Howardi ◽  
Southern Corn Rootworm ◽  
Trap Cropping

Field trials to test the efficacy of trap cropping and biological control for the management of Diabrotica undecimpunctata howardi Barber (Chrysomelidae: Luperini) in peanuts were conducted in 1992, 1993, and 1994. Cucurbita maxima Duchesne cv. ‘Blue Hubbard’ was used as a trap crop for adult beetles and the entomopathogenic nematodes, Steinernema carpocapse Weiser and Steinernema riobravis Cabanillas, Poinar and Raulston, were used as biological control agents against soil-inhabiting larvae. In 1992, peanut yields were highest in treatments that included a trap crop. Trap crop did not affect yield in 1993 or 1994. In 2 out of the 3 years, distribution of pod damage relative to the trap crop suggested that beetles oviposited more frequently in peanuts growing in the row next to the trap crop than in peanuts 3 rows from the trap crop. Although entomopathogenic nematodes persisted for a sufficient period to overlap with the presence of rootworms, they did not affect yield or pod damage in peanuts.

scholarly journals Evaluation of two native entomopathogenic nematodes against Odontotermes obesus (Rambur) (Isoptera: Termitidae) and Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
K. Sindhura Bhairavi ◽  
Badal Bhattacharyya ◽  
Gitanjali Devi ◽  
Sudhansu Bhagawati ◽  
Partha Pratim Gyanudoy Das ◽  
...  
Keyword(s):  
Biological Control ◽  
Mortality Rate ◽  
Entomopathogenic Nematodes ◽  
Heterorhabditis Bacteriophora ◽  
Agrotis Ipsilon ◽  
Biological Control Agents ◽  
Laboratory Conditions ◽  
Odontotermes Obesus ◽  
Percent Mortality ◽  
Lepidoptera Noctuidae

Abstract Background Entomopathogenic nematodes (EPNs) are one of the widely studied biological control agents. The present study was conducted to evaluate the efficacy of two EPNs species, Heterorhabditis bacteriophora (Poinar) (Rhabditida: Heterorhabditidae) and Steinernema aciari (Qui, Yan, Zhou, Nguyen and Pang) (Rhabditida: Steinernematidae), isolated locally from soils of Majuli river island, Assam, India against two important subterrenean pests; Odontotermes obesus (Rambur) (Isoptera: Termitidae) and Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) under laboratory conditions. Results In case of O. obesus, mortality percent was recorded by H. bacteriophora after 72 h. at 300 IJs/termite and by S. aciari at 250 and 300 IJs/termite after 96 h. The lowest LD50 and LT50 values obtained for H. bacteriophora were 13.054 IJs/termite and 26.639 h., respectively, while those of S. aciari were 42.040 IJs/termite and 31.761 h., respectively. With respect to A. ipsilon, H. bacteriophora registered a highest mortality rate at 300 IJs/larvae after 144 h. S. aciari showed 100 percent mortality at 300 IJs/larva after 168 h. The lowest values of LD50 and LT50 for H. bacteriophora were 35.711 IJs/larva and 83.050 h., respectively. The lowest values of LD50 and LT50 for S. aciari were 71.192 IJs/larvae and 97.921 h., respectively. Overall, H. bacteriophora displayed more virulence toward O. obesus and A. ipsilon than S. aciari. Conclusion Both native EPNs were found effective against O. obesus and A. ipsilon. However, H. bacteriophora was more virulent toward O. obesus and A. ipsilon than S. aciari under the laboratory conditions.

scholarly journals A survey investigating the diversity and distribution of entomopathogenic nematodes in the UK and the first confirmed UK record of Steinernema carpocapsae

Nematology ◽  
2018 ◽  
Vol 20 (9) ◽  
pp. 851-858 ◽  
Author(s):  
Cassandra Edmunds ◽  
Rory J. Post ◽  
Craig S. Wilding ◽  
Robbie Rae
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Soil Samples ◽  
Steinernema Carpocapsae ◽  
Biological Control Agents ◽  
Diverse Range ◽  
The World ◽  
The Uk ◽  
Insect Parasites

Summary Entomopathogenic nematodes (EPN) of the families Steinernematidae and Heterorhabditidae are lethal insect parasites that have been commercialised as biological control agents. EPN have been isolated from across the world but it has been more than 20 years since the last survey of the UK, and species like Steinernema carpocapsae have never been found here and positively identified through molecular biology. We collected 518 soil samples from a diverse range of habitats across the UK and baited them with Galleria mellonella to isolate EPN. Dead G. mellonella were placed in White traps and emergent EPN underwent DNA barcoding analyses. From the 518 samples, 3.5% were positive for EPN. No Heterorhabditis species were found, but seven isolates of S. glaseri, one isolate of S. feltiae, eight isolates of S. affine and two isolates of S. carpocapsae were found. This was the first confirmed record of S. carpocapsae in the UK.

scholarly journals Biological Control Entomopathogenic Nematodes Heterorhabditis sp. and Steinernema sp. Pest Control Termite Land As Coptotermes sp. and Microtermes sp. in The District Lumajang

2015 ◽  
Vol 16 (1) ◽  
pp. 43
Author(s):  
Qodiriyah Qodiriyah ◽  
Didik Sulistyanto ◽  
Purwatiningsih Purwatiningsih
Keyword(s):  
Biological Control ◽  
Pest Control ◽  
Entomopathogenic Nematodes ◽  
Probit Analysis ◽  
Effective Concentration ◽  
Subterranean Termites ◽  
Pathogenicity Test ◽  
Biological Control Agents ◽  
Effective Time ◽  
Mortality Percentage

This research aims to find out the most effective concentration (LC50) and the most effective time (LT50)  and  to select a biological agent netamoda of nematodes Steinernema sp. dan Heterorhabditis sp. as the environmentally friendly biological control agents on the ground termite Coptotermes sp. and   Microtermes sp. in Lumajang. All the data were analyzed by using the termite mortality percentage variance analysis. The LC50 and LT50 were tested by using the probit analysis. Therefore, the empirical probit values were obtained from the percentage of mortality after they were calculated by the Abbot formulation. The biological agents netamoda entomopathogenic Heterorhabditis sp. and Steinernema sp. had the high value on the pathogenicity of Coptotermes sp. and Microtermes sp., the LC50 values on Coptotermes sp. of nematodes Heterorhabditis sp. was 14.94 IJ/ml and Steinernema sp. was 15.22 IJ/ml., the LC50 values on Microtermes sp. of nematodes Heterorhabditis sp. was 16.54 IJ/ml and  Steinernema sp. was 20.39 IJ/ml., the LT50 values on Coptotermes sp. of nematodes Heterorhabditis sp. was 1.29 hours and Steinernema sp. was 1.35 hours, and the LT50 values on Microtermes sp. of nematodes Heterorhabditis sp. was 2.14 hours and Steinernema sp. was 1.82 hours. Through pathogenicity test, the entomopathogenic nematodes Heterorhabditis sp. is more effective to control subterranean termites compared with the nematode Steinernema sp. This happens because Heterorhabditis sp. are actively engaged and looking for a host so that there are a large number of possibilities of contacting them with a very large subterranean termites.Keywords: Entomopathogenic nematodes,   Heterorhabditis sp., Steinernema sp., LC50, LT50 

scholarly journals MASS PRODUCTION OF ENTOMOPATHOGENIC NEMATODES OF LOCAL ISOLATES AS BIOLOGICAL CONTROL AGENTS OF COFFEE BERRY BORER (Hypothenemus hampei Ferr.)

2019 ◽  
Vol 19 (1) ◽  
pp. 8
Author(s):  
Wagiyana Wagiyana ◽  
Didik Sulistyanto ◽  
Joko Waluyo
Keyword(s):  
Biological Control ◽  
Mass Production ◽  
Entomopathogenic Nematodes ◽  
Infective Juvenile ◽  
Hypothenemus Hampei ◽  
Biological Control Agents ◽  
Coffee Berry ◽  
Coffee Plantations

Entomopathogenic Nematodes (EpNs) that serve as biological control agents include Steinernema spp and  Heterorhabditis spp. EPNs Heterorhabditis indicus (Bromo Isolate) has a high toxicity against larvae Lepidoptera and Coleoptera and was successfully developed for mass production in Biological Control Laboratory of Jember University. These nematodes will be formulated as solid and liquid biopesticides. This research aims: to find EPNs local isolates from smallholder coffee plantations; to identify local and in vivo and in vitro cultured EPNs isolates; to determine the pathogenicity of EpNs local isolate against Coffee Berry Borrer (CBB) Hypothenemus hampei Ferr and to formulate EpNs in vitro culture as a liquid and solid formula. These formula were tested for the patogenicity and viability of EpNs to the larvae of H. hampei, Tenebrio molitor and Galleria melonella. The results showed that the pathogenecity of EpNs isolate to the larvae of CBB in Silo was 30% after 24 hours and 90% after 48 hours of in vivo inoculation. However, the mortality of CBB larvae was only 10% by liquid spraying on the coffee berry. The viability was 524 IJ (Infective Juvenile) on liquid formula packed on polyurethane sponge, and this was higher than that on solid formula (330 IJ).

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