Transcriptomic response of peripheral blood mononuclear cells to secukinumab in an 8‐year‐old boy with juvenile generalized pustular psoriasis

2022 ◽  
Author(s):  
Ping‐Chen Hou ◽  
Wilson Jr. Aala ◽  
Yu‐Rong Lee ◽  
Bryan Edgar K. Guevara ◽  
Peng‐Chieh Chen ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Pustular Psoriasis ◽  
Peripheral Blood Mononuclear ◽  
Transcriptomic Response ◽  
Generalized Pustular Psoriasis ◽  
Blood Mononuclear Cells

scholarly journals Transcriptomic changes in peripheral blood mononuclear cells with weight loss: systematic literature review and primary data synthesis

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Kaitlin Day ◽  
Aimee L. Dordevic ◽  
Helen Truby ◽  
Melissa C. Southey ◽  
Susan Coort ◽  
...  
Keyword(s):  
Gene Expression ◽  
Weight Loss ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Transcriptomic Response ◽  
Low Responders ◽  
Blood Mononuclear Cells ◽  
Transcriptomic Changes

Abstract Background and objectives Peripheral blood mononuclear cells (PBMCs) have shown promise as a tissue sensitive to subtle and possibly systemic transcriptomic changes, and as such may be useful in identifying responses to weight loss interventions. The primary aim was to comprehensively evaluate the transcriptomic changes that may occur during weight loss and to determine if there is a consistent response across intervention types in human populations of all ages. Methods Included studies were randomised control trials or cohort studies that administered an intervention primarily designed to decrease weight in any overweight or obese human population. A systematic search of the literature was conducted to obtain studies and gene expression databases were interrogated to locate corresponding transcriptomic datasets. Datasets were normalised using the ArrayAnalysis online tool and differential gene expression was determined using the limma package in R. Over-represented pathways were explored using the PathVisio software. Heatmaps and hierarchical clustering were utilised to visualise gene expression. Results Seven papers met the inclusion criteria, five of which had raw gene expression data available. Of these, three could be grouped into high responders (HR, ≥ 5% body weight loss) and low responders (LR). No genes were consistently differentially expressed between high and low responders across studies. Adolescents had the largest transcriptomic response to weight loss followed by adults who underwent bariatric surgery. Seven pathways were altered in two out of four studies following the intervention and the pathway ‘cytoplasmic ribosomal proteins’ (WikiPathways: WP477) was altered between HR and LR at baseline in the two datasets with both groups. Pathways related to ‘toll-like receptor signalling’ were altered in HR response to the weight loss intervention in two out of three datasets. Conclusions Transcriptomic changes in PBMCs do occur in response to weight change. Transparent and standardised data reporting is needed to realise the potential of transcriptomics for investigating phenotypic features. Registration number PROSPERO: CRD42019106582

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