Hyaluronic acid biomaterial for human tissue‐engineered skin substitutes: Preclinical comparative in vivo study of wound healing

2020 ◽  
Vol 34 (10) ◽  
pp. 2414-2427 ◽  
Author(s):  
Á. Sierra‐Sánchez ◽  
A. Fernández‐González ◽  
A. Lizana‐Moreno ◽  
O. Espinosa‐Ibáñez ◽  
A. Martinez‐Lopez ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Human Tissue ◽  
In Vivo Study ◽  
Skin Substitutes ◽  
Engineered Skin Substitutes

13861 Human tissue engineered skin substitutes based on hyaluronic acid for wound healing: Epidermal barrier study

2020 ◽  
Vol 83 (6) ◽  
pp. AB125
Author(s):  
Álvaro Sierra-Sánchez ◽  
Antonio Lizana Moreno ◽  
Ana Fernández-González ◽  
Olga Espinosa-Ibáñez ◽  
Antonio Martinez-Lopez ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Human Tissue ◽  
Skin Substitutes ◽  
Epidermal Barrier ◽  
Engineered Skin Substitutes

Anti-inflammatory and wound healing properties of Rubus fairholmianus Gard. root—An in vivo study

2014 ◽  
Vol 54 ◽  
pp. 216-225 ◽  
Author(s):  
Blassan P. George ◽  
Thangaraj Parimelazhagan ◽  
Rahul Chandran
Keyword(s):  
Wound Healing ◽  
In Vivo Study ◽  
Anti Inflammatory

scholarly journals Invasive Techniques in Scar Management: Skin Substitutes

2020 ◽  
pp. 317-323
Author(s):  
F. W. Timmermans ◽  
E. Middelkoop
Keyword(s):  
Wound Healing ◽  
Scar Formation ◽  
Wound Management ◽  
Skin Substitutes ◽  
Current State ◽  
Scar Management ◽  
Scar Quality ◽  
Engineered Skin Substitutes ◽  
Invasive Techniques

AbstractIn the last decades, skin substitutes have emerged as an important innovation in improving scar quality. They can be applied during the initial wound management but also during scar reconstruction procedures. This chapter provides an overview on the development, current state, and future of cell-seeded and tissue-engineered skin substitutes. We will discuss some of the most important varieties of skin substitutes in the context of scar formation and wound healing.

Enhanced wound healing by PVA/Chitosan/Curcumin patches: In vitro and in vivo study

2019 ◽  
Vol 182 ◽  
pp. 110339 ◽  
Author(s):  
Niranjan R. ◽  
Kaushik M. ◽  
Prakash J. ◽  
Venkataprasanna K.S. ◽  
Christy Arpana ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Study

scholarly journals Adipose Tissue-Derived Mesenchymal Cells Support Skin Reepithelialization through Secretion of KGF-1 and PDGF-BB: Comparison with Dermal Fibroblasts

2012 ◽  
Vol 21 (11) ◽  
pp. 2441-2454 ◽  
Author(s):  
Vassilia-Ismini Alexaki ◽  
Despoina Simantiraki ◽  
Marianna Panayiotopoulou ◽  
Olga Rasouli ◽  
Maria Venihaki ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adipose Tissue ◽  
Normal Skin ◽  
Human Adipose Tissue ◽  
Mesenchymal Cells ◽  
Dermal Fibroblasts ◽  
Skin Substitutes ◽  
Keratinocyte Proliferation

Epidermal organization and homeostasis are regulated by mesenchymal influences through paracrine actions. Until today, dermal fibroblasts (DFs) are used in the “dermal” layer to support keratinocyte growth in vitro in dermal and skin substitutes. In the present work, we used human adipose tissue-derived mesenchymal cells (ADMCs) as a support of keratinocyte growth in vitro (in monolayer culture and in 3D skin cell culture models) and in vivo (mouse wound healing models) and compared our findings with those obtained using dermal fibroblasts. ADMCs induce reepithelialization during wound healing more efficiently than DFs, by enhancing keratinocyte proliferation through cell cycle progression, and migration. This effect is mediated (at least partially) by a paracrine action of KGF-1 and PDGF-BB, which are more prominently expressed in ADMCs than in DFs. Furthermore, replacement of DFs by ADMCs in the dermal compartment of organotypic skin cultures leads to an artificial epidermis resembling to that of normal skin, concerning the general histology, although with a higher expression of cytokeratins 5 and 19. In Rag1 knockout mice, ADMCs induced a more rapid reepithelialization and a more effective wound healing, compared to dermal fibroblasts. In conclusion, we provide evidence that ADMCs can serve as supportive cells for primary keratinocyte cultures. In addition, because of their abundance and the great cell yield achieved during ADMC isolation, they represent an interesting cell source, with potential aspects for clinical use.

Silver Sulphadiazine- xanthan gum- hyaluronic Acid Composite Hydrogel for Wound Healing: Formulation Development and in vivo Evaluation

2020 ◽  
Vol 16 (1) ◽  
pp. 21-29 ◽  
Author(s):  
M.O. Ilomuanya ◽  
Z.A. Seriki ◽  
U.N. Ubani-Ukoma ◽  
B.A. Oseni ◽  
B.O. Silva
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Xanthan Gum ◽  
Wound Closure ◽  
Antimicrobial Agents ◽  
Biological Properties ◽  
Formulation Development ◽  
Hybrid Hydrogels ◽  
Silver Sulphadiazine

Background: Development and modifications of hybrid hydrogels have been done to improve biological properties or to decrease the disadvantages of biomaterials.Objectives: The efficacy of hyaluronic acid in combination with silver sulphadiazine in wound healing was investigated. The retaining properties of xanthan gum to aid re- epithelialization was also explored.Materials and Method: Four hybrid hydrogels comprising of different concentrations of xanthan gum, eugenol and antimicrobial agents – hyaluronic acid and silver sulphadiazine were formulated. The physicochemical properties of the gels were assessed, and the antimicrobial effectiveness of the different hydrogel were determined using the extent of wound closure as an index.Results: The hydrogel samples had approximately 90% moisture content with rate of evaporation between 26- 32% for a 5 h period at 37oC. The pH of all formulations was between 7.59 - 8.05 considering that the formulation would be applied to underlying tissues of the skin. The swelling index after a 12 h period in distilled water was 10% for HX 1, 27% for HX 2, 29% for HX 3 and 30% for HX 4. There was no new peak observed in the FTIR analysis to indicate formation of new bonds.Conclusion: Incorporation of silver sulphadiazine at 0.1% and hyaluronic acid at 1.5% in the formulation yielded the best results with regards to least presence of inflammatory cell infiltrates and excellent wound closure at 14 days compared to the control and other formulations. Further investigation may be required for clinical use as an effective wound dressing material. Keywords: Silver sulphadiazine, Xanthan gum, Hyaluronic acid, Hydrogels, Wound healing.

scholarly journals Comparative study between the effects of hyaluronic acid and acid galactan purified from eggs of the mollusk Pomacea sp in wound healing

2004 ◽  
Vol 19 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Ana Katarina Menezes da Cruz ◽  
Wogelsanger Oliveira Pereira ◽  
Elizeu Antunes dos Santos ◽  
Maria Goretti Freire Carvalho ◽  
Aldo da Cunha Medeiros ◽  
...  
Keyword(s):  
Wound Healing ◽  
Small Intestine ◽  
Hyaluronic Acid ◽  
Wistar Rats ◽  
Saline Solution ◽  
Giant Cells ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Healing Tissue

PURPOSE: To compare the effect of hyaluronic acid (HA) and of AG on the healing of intestine wounds. METHODS: The semi-purified extract of the eggs of the mollusc was obtained by fractionation with ammonium sulfate and purification for ion-exchange chromatography. The obtained galactans were eluted in water (neutral galactan) and in 0.1 and 0.2M NaCl (acidic galactans). The in vivo study was performed with 45 "Wistar" rats, separated in three groups (n=15). Solutions containing HA 1%, GA 1% or saline solution 0,9%, was placed topically on the sutures of wounds in the small intestine of the rats. After 05, 10 and 21 days the animals were sacrificed and biopsy of the healing tissue was done. RESULTS: The hystologic grading was more significant for HA and AG groups when compared to the group C. AG stimulated the appearance of macrophages, giant cells and increase in the concentration of collagen in the area of the wound when compared to HA. CONCLUSION: The topical use of GA in intestinal wounds promoted the anticipation of events that are important in the wound healing.

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