scholarly journals JAK1/3 inhibition preserves epidermal morphology in full-thickness 3D skin models of atopic dermatitis and psoriasis

2019 ◽  
Vol 33 (2) ◽  
pp. 367-375 ◽  
Author(s):  
K. Clarysse ◽  
C.M. Pfaff ◽  
Y. Marquardt ◽  
L. Huth ◽  
I. Kortekaas Krohn ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Full Thickness ◽  
3D Skin ◽  
Epidermal Morphology

scholarly journals 299 Induction of an atopic dermatitis-like phenotype in a full-thickness in vitro human skin model

2020 ◽  
Vol 140 (7) ◽  
pp. S36
Author(s):  
J. Molignano ◽  
J. Oldach ◽  
K. Marengo ◽  
S. Ayehunie ◽  
M. Klausner
Keyword(s):  
Atopic Dermatitis ◽  
Human Skin ◽  
Full Thickness ◽  
Skin Model

scholarly journals Osthole Inhibits Expression of Genes Associated with Toll-like Receptor 2 Signaling Pathway in an Organotypic 3D Skin Model of Human Epidermis with Atopic Dermatitis

Cells ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 88
Author(s):  
Natalia Karolina Kordulewska ◽  
Justyna Topa ◽  
Robert Stryiński ◽  
Beata Jarmołowska
Keyword(s):  
Atopic Dermatitis ◽  
Signaling Pathway ◽  
Genetic Profile ◽  
Toll Like Receptor ◽  
Skin Model ◽  
Barrier Dysfunction ◽  
Gene Expressions ◽  
Expression Of Genes ◽  
3D Skin

The Toll-like receptor (TLR) family signature has been linked to the etiopathology of atopic dermatitis (AD), a chronic inflammatory skin disease associated with skin barrier dysfunction and immune system imbalance. We aimed to investigate whether osthole (a plant-derived compound) can inhibit the genetic profile of key genes associated with TLR2 signaling (TIRAP, MyD88, IRAK1, TRAF6, IκBα, NFκB) after stimulation with LPS or histamine in a 3D in vitro model of AD. Overexpression of the aforementioned genes may directly increase the secretion of proinflammatory cytokines (CKs) and chemokines (ChKs), which may exacerbate the symptoms of AD. Relative gene expressions were quantified by qPCR and secretion of CKs and ChKs was evaluated by ELISA assay. LPS and histamine increased the relative expression of genes related to the TLR2 pathway, and osthole successfully reduced it. In summary, our results show that osthole inhibits the expression of genes associated with the TLR signaling pathway in a skin model of AD. Moreover, the secretion of CKs and ChKs after treatment of AD with osthole in a 3D skin model in vitro suggests the potential of osthole as a novel compound for the treatment of AD.

scholarly journals LB1583 Induction of an atopic dermatitis-like phenotype in a full-thickness in vitro human skin model

2018 ◽  
Vol 138 (9) ◽  
pp. B20
Author(s):  
J. Molignano ◽  
K. Marengo ◽  
J. Oldach ◽  
G. Stolper ◽  
M. Li ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Human Skin ◽  
Full Thickness ◽  
Skin Model

scholarly journals 726 Induction of an atopic dermatitis-like phenotype in a full-thickness in vitro human skin model

2019 ◽  
Vol 139 (5) ◽  
pp. S124
Author(s):  
P. Hayden ◽  
S. Ayehunie ◽  
J. Oldach ◽  
M. Klausner
Keyword(s):  
Atopic Dermatitis ◽  
Human Skin ◽  
Full Thickness ◽  
Skin Model

scholarly journals Effect of Resveratrol-Enriched Rice on Skin Inflammation and Pruritus in the NC/Nga Mouse Model of Atopic Dermatitis

2019 ◽  
Vol 20 (6) ◽  
pp. 1428 ◽  
Author(s):  
Min Kang ◽  
Kyohee Cho ◽  
Jae Lee ◽  
Lalita Subedi ◽  
Silvia Yumnam ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Immunoglobulin E ◽  
Alternative Therapy ◽  
Human Keratinocytes ◽  
Skin Inflammation ◽  
Skin Lesions ◽  
Enzyme Linked Immunosorbent Assay ◽  
Skin Model ◽  
3D Skin

Resveratrol-enriched rice (RR) was developed using genetic engineering to combine the properties of resveratrol and rice. To evaluate the effect of RR on pruritic skin inflammation in atopic dermatitis (AD)-like skin lesions, we used dinitrochlorobenzene (DNCB)-induced NC/Nga mice and an in vitro 3D skin model. Normal rice (NR), resveratrol, and RR were topically applied to mice dorsal skin, following which the dermatitis index and scratching frequency were calculated. Histological examination was performed by hematoxylin and eosin and immunohistochemistry staining of IL-31 level. The level of immunoglobulin E (IgE) and IL-31 in the serum was determined by enzyme-linked immunosorbent assay (ELISA). The cytotoxicity of RR and the expression levels of pro-inflammatory cytokines were also determined in cultured human keratinocytes and a 3D skin model. RR significantly reduced scratching frequency, decreased the dermatitis severity and trans-epidermal water loss (TEWL) and improved skin hydration in DNCB-induced NC/Nga mice. RR also significantly decreased serum IL-31 and IgE levels and suppressed the production of IL-6 in human keratinocytes and the 3D skin model. Our study indicates that the synergistic effect of rice and resveratrol manifested by the topical application of RR can serve as a potential alternative therapy for chronic skin inflammatory diseases such as AD.

scholarly journals Topical therapy in canine atopic dermatitis: new products

2020 ◽  
Vol 25 (2) ◽  
pp. 76-82
Author(s):  
Tim Nuttall
Keyword(s):  
Atopic Dermatitis ◽  
Biofilm Formation ◽  
Topical Therapy ◽  
Residual Activity ◽  
Canine Atopic Dermatitis ◽  
Topical Product ◽  
Ophiopogon Japonicus ◽  
Improve Compliance ◽  
Epidermal Morphology ◽  
The Ideal

Topical therapy in canine atopic dermatitis (CAD) can moisturise and soothe the skin; treat and prevent secondary bacterial and yeast infections; and remove debris, grease and allergens. Shampoos are commonly used but can be time-consuming. Non-rinsing wipes, foams and spot-on preparations facilitate topical application and improve compliance. The ideal topical product for CAD should be emollient, lipid-barrier sparing, microbiome balancing, easy to apply and well-tolerated, have some residual activity, and be safe for the dog and owner. Ophytrium is a novel Ophiopogon japonicus (Japanese mondo grass) derived product. It blocks inflammatory pathways, maintains epidermal morphology and barrier function, and reduces staphylococcal adherence and biofilm formation in human and canine epidermis models. It has been incorporated in shampoos and foams for dogs (Douxo S3®; Ceva Sante Animale, Libourne, France). Use of 3-week treatment cycles with one shampoo followed by a foam application every 2–3 days helps to ameliorate CAD (Douxo S3 Calm®) and superficial staphylococcal pyoderma (ophytrium/3% chlorhexidine; Douxo S3 Pyo®). Effective topical therapy used alone or alongside other modalities can improve the effectiveness and safety of treatment for CAD. Reducing the need for systemic antimicrobials is a vital part of antimicrobial stewardship.

Use of mass spectrometry imaging and a full thickness 3D skin equivalent for evaluation of percutaneous absorption

2017 ◽  
Vol 280 ◽  
pp. S272
Author(s):  
Michael Bachelor ◽  
Erin Seeley ◽  
Callee Walsh ◽  
Alex Armento ◽  
Mitchell Klausner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Percutaneous Absorption ◽  
Mass Spectrometry Imaging ◽  
Skin Equivalent ◽  
Full Thickness ◽  
3D Skin
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