scholarly journals Highly abundant defense proteins in human sweat as revealed by targeted proteomics and label-free quantification mass spectrometry

2015 ◽  
Vol 29 (10) ◽  
pp. 2024-2031 ◽  
Author(s):  
É. Csősz ◽  
G. Emri ◽  
G. Kalló ◽  
G. Tsaprailis ◽  
J. Tőzsér
Keyword(s):  
Mass Spectrometry ◽  
Targeted Proteomics ◽  
Label Free ◽  
Defense Proteins ◽  
Label Free Quantification ◽  
Human Sweat ◽  
Free Quantification

scholarly journals Host defense responses of CO441 and CL30 maize lines to Fusarium graminearum analyzed by comparative label-free quantitative proteomics

2019 ◽  
Author(s):  
Lana M. Reid ◽  
Illimar Altosaar
Keyword(s):  
Mass Spectrometry ◽  
Fusarium Graminearum ◽  
Fungal Pathogen ◽  
Detailed Knowledge ◽  
Label Free ◽  
Maize Line ◽  
Defense Proteins ◽  
Label Free Quantification ◽  
Host Pathogen ◽  
Free Quantification

AbstractGibberella ear rot is a disease of maize associated with low yields and the production of harmful mycotoxins therein. The disease is caused by the infection of host Zea mays with fungal pathogen Fusarium graminearum. Resistant (CO441) and susceptible (CL30) inbred maize line kernels were inoculated with conidial suspensions of F. graminearum or water (controls). Ears of maize (cobs) from each line were harvested upon maturation and proteins were extracted from the embryo tissue of the kernels to study tissue-specific response of the host. Embryo proteins from both CO441 and CL30 lines were sequenced using mass spectrometry (LC-MS/MS) and quantified using Label Free Quantification (LFQ). Following filtering, 509 proteins were identified. These proteins were grouped into nine functional categories: Fusarium-derived, late embryogenesis abundant, oil-body, metabolism, stress, cellular, protein storage, metabolism, and defense. Defense proteins were up-regulated in response to infection in both CO441 and CL30 lines. Furthermore, F. graminearum derived proteins were only found in CL30 infected kernels suggesting that resistance may be attributed in part to the inability of Fusarium to establish itself in the embryo. To our knowledge this is the first successful application of LFQ mass spectrometry to the study of host-pathogen response to F. graminearum.Biological significanceFungal pathogen Fusarium graminearum is responsible for billion dollar losses in crops and contamination of global grains with harmful mycotoxins. By studying host-pathogen interactions of Fusarium and maize on a proteomic level with resistant and susceptible genotypes, the biological interactions occurring during infection of the maturing seed were characterized. Mature kernels of the F. graminearum susceptible maize line CL30 and resistant CO441 line were dissected to permit a proteomic survey of the new sporophytic generation, the embryo. Detailed knowledge of this Host-pathogen interactome will assist development of new cereal lines resistant to the rot diseases caused by Fusarium graminearum.HighlightsSusceptible (CL30) and Resistant (CO441) lines were injected with water mock or F. graminearum LC-MS/MS of maize embryo protein extracts followed by Label Free Quantification (LFQ) permitted identification, quantification and comparison of proteomes between maize genotypes and treatments Fusarium-derived proteins were abundant only in the susceptible infected embryo Defense proteomes were up-regulated in both lines following infection nsLTP and Protease Inhibitor were significantly over-expressed in the Susceptible line after infection; chitinase and WIP1 were significantly over-expressed in the Resistant line after infection

Label-Free Quantification by Data Independent Acquisition Mass Spectrometry to Map Cardiovascular Proteomes

2016 ◽  
pp. 227-245
Author(s):  
Sarah J. Parker ◽  
Ronald J. Holewinski ◽  
Irina Tchernyshyov ◽  
Vidya Venkatraman ◽  
Laurie Parker ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Label Free ◽  
Label Free Quantification ◽  
Data Independent Acquisition ◽  
Free Quantification

Mass spectrometry-based label free quantification of gel separated proteins

2012 ◽  
Vol 75 (18) ◽  
pp. 5544-5553 ◽  
Author(s):  
Zoltan Szabo ◽  
Judit Szeline Szomor ◽  
Istvan Foeldi ◽  
Tamas Janaky
Keyword(s):  
Mass Spectrometry ◽  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification
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