Non-invasive short-term assessment of retinoids effects on human skin in vivo using multiphoton microscopy

2014 ◽  
Vol 29 (4) ◽  
pp. 673-681 ◽  
Author(s):  
E. Tancrède-Bohin ◽  
T. Baldeweck ◽  
E. Decencière ◽  
S. Brizion ◽  
S. Victorin ◽  
...  
2021 ◽  
Author(s):  
Peter R. Corridon

AbstractThe aim of the present study was to determine whether decellularized rat kidney microvascular and extracellular matrix (ECM) integrity could be preserved under in vivo conditions directly after transplantation. Whole kidneys were harvested from the Sprague Dawley rat and were decellularized by perfusion with 0.5% sodium dodecyl sulfate (SDS) for 24 hours, followed by phosphate-buffered saline (PBS) for an additional 24 hours. Decellularized kidneys were then transplanted into recipients and vascular high-molecular-weight (150-kDa) FITC dextrans were infused via the jugular vein. Blood was then allowed to flow through the decellularized transplant. Intravital multiphoton microscopy confirmed the suitable confinement of the dextrans within vascular tracks and preservation of the decellularized architecture that was monitored in the shortterm post transplantation.New and NoteworthyThe study confirmed in vivo microvascular and ECM preservation in the short-term post transplantation.


Author(s):  
Mihaela Balu ◽  
Griffin Lentsch ◽  
Dorota Korta ◽  
Karsten Konig ◽  
Kristen M. Kelly ◽  
...  

2019 ◽  
Vol 91 (9) ◽  
pp. 1481-1491 ◽  
Author(s):  
Leszek Kubisz ◽  
Dorota Hojan-Jezierska ◽  
Maria Szewczyk ◽  
Anna Majewska ◽  
Weronika Kawałkiewicz ◽  
...  

Abstract Structural and chemical alterations in living tissue are reflected in electrical impedance changes. However, due to the complexity of skin structure, the relation between electrical parameters and physiological/pathological conditions is difficult to establish. The impedance dispersion reflects the clinical status of the examined skin tissue and, therefore, it is frequently used in a non-invasive evaluation of exposing skin to various factors. The method has been used to assess the effect of the fish collagen on the skin of patients suffering from the leg ulcer. Therefore, from a number of different approaches to skin electrical impedance dispersion, the one considered to be safe was selected and applied. This paper presents a short review of different technical approaches to in vivo electrical impedance measurements, as well as an analysis of the results and the effect of fish collagen locally administered on human skin.


2020 ◽  
Vol 26 (6) ◽  
pp. 794-803
Author(s):  
Emmanuelle Tancrède‐Bohin ◽  
Thérèse Baldeweck ◽  
Sébastien Brizion ◽  
Etienne Decencière ◽  
Steeve Victorin ◽  
...  

2007 ◽  
Author(s):  
Ronan Le Harzic ◽  
Anne Colonna ◽  
Rainer Bückle ◽  
Alexander Ehlers ◽  
Christophe Hadjur ◽  
...  

2005 ◽  
Author(s):  
Karsten König ◽  
Iris Riemann ◽  
Alexander Ehlers ◽  
Ronan Le Harzic

2011 ◽  
Author(s):  
Volker Huck ◽  
Christian Gorzelanny ◽  
Kai Thomas ◽  
Christian Mess ◽  
Valentina Dimitrova ◽  
...  

Author(s):  
Jianghong Yuan ◽  
Canan Dagdeviren ◽  
Yan Shi ◽  
Yinji Ma ◽  
Xue Feng ◽  
...  

Conformal modulus sensors (CMS) incorporate PZT nanoribbons as mechanical actuators and sensors to achieve reversible conformal contact with the human skin for non-invasive, in vivo measurements of skin modulus. An analytic model presented in this paper yields expressions that connect the sensor output voltage to the Young moduli of the epidermis and dermis, the thickness of the epidermis, as well as the material and geometrical parameters of the CMS device itself and its encapsulation layer. Results from the model agree well with in vitro experiments on bilayer structures of poly(dimethylsiloxane). These results provide a means to determine the skin moduli (epidermis and dermis) and the thickness of the epidermis from in vivo measurements of human skin.


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