scholarly journals Clinical and genetic determinants of urinary glucose excretion in patients with diabetes mellitus

2020 ◽  
Author(s):  
Keisuke Monobe ◽  
Shinsuke Noso ◽  
Naru Babaya ◽  
Yoshihisa Hiromine ◽  
Yasunori Taketomo ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Genetic Determinants ◽  
Urinary Glucose Excretion ◽  
Urinary Glucose ◽  
Patients With Diabetes ◽  
Glucose Excretion

scholarly journals Dapagliflozin Aggravates Renal Injury via Promoting Gluconeogenesis in db/db Mice

2018 ◽  
Vol 45 (5) ◽  
pp. 1747-1758 ◽  
Author(s):  
Yingli Jia ◽  
Jinzhao He ◽  
Liang Wang ◽  
Limin Su ◽  
Lei Lei ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
Renal Impairment ◽  
Glucose Tolerance ◽  
Blood Urea Nitrogen ◽  
Urinary Glucose Excretion ◽  
Urea Nitrogen ◽  
Urinary Glucose ◽  
Glucose Excretion

Background/Aims: A sodium-glucose co-transporter-2 inhibitor dapagliflozin is widely used for lowering blood glucose and its usage is limited in type 2 diabetes mellitus patients with moderate renal impairment. As its effect on kidney function is discrepant and complicated, the aim of this study is to determine the effect of dapagliflozin on the progression of diabetic nephropathy and related mechanisms. Methods: Twelve-week-old male C57BL/6 wild-type and db/db mice were treated with vehicle or 1 mg/kg dapagliflozin for 12 weeks. Body weight, blood glucose, insulin tolerance, glucose tolerance, pyruvate tolerance and 24-hour urine were measured every 4 weeks. At 24 weeks of age, renal function was evaluated by blood urea nitrogen level, creatinine clearance, urine output, urinary albumin excretion, Periodic Acid-Schiff staining, Masson’s trichrome staining and electron microscopy. Changes in insulin signaling and gluconeogenic key regulatory enzymes were detected using Western blot analysis. Results: Dapagliflozin did not alleviate but instead aggravated diabetic nephropathy manifesting as increased levels of microalbuminuria, blood urea nitrogen, and glomerular and tubular damage in db/db mice. Despite adequate glycemic control by dapagliflozin, urinary glucose excretion increased after administration before 24 weeks of age and was likely associated with renal impairment. Increased urinary glucose excretion was mainly derived from the disturbance of glucose homeostasis with elevated hepatic and renal gluconeogenesis induced by dapagliflozin. Although it had no effect on insulin sensitivity and glucose tolerance, dapagliflozin further induced the expression of gluconeogenic key rate-limiting enzymes through increasing the expression levels of FoxO1 in the kidney and liver. Conclusion: These experimental results indicate that dapagliflozin aggravates diabetes mellitus-induced kidney injury, mostly through increasing gluconeogenesis.

155-LB: The Increase in Endogenous Glucose Production with SGLT2 Inhibition Is Unchanged by Renal Denervation but Highly Correlates to Urinary Glucose Excretion

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 155-LB
Author(s):  
CAROLINA SOLIS-HERRERA ◽  
MARIAM ALATRACH ◽  
CHRISTINA AGYIN ◽  
HENRI HONKA ◽  
RUPAL PATEL ◽  
...  
Keyword(s):  
Renal Denervation ◽  
Glucose Production ◽  
Endogenous Glucose Production ◽  
Urinary Glucose Excretion ◽  
Urinary Glucose ◽  
Sglt2 Inhibition ◽  
Endogenous Glucose ◽  
Glucose Excretion

scholarly journals Morning Spot Urine Glucose-to-Creatinine Ratios Predict Overnight Urinary Glucose Excretion in Patients With Type 2 Diabetes

2017 ◽  
Vol 37 (1) ◽  
pp. 9-17 ◽  
Author(s):  
So Ra Kim ◽  
Yong-ho Lee ◽  
Sang-Guk Lee ◽  
Sun Hee Lee ◽  
Eun Seok Kang ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Urinary Glucose Excretion ◽  
Urine Glucose ◽  
Spot Urine ◽  
Urinary Glucose ◽  
Glucose Excretion

Abnormal renal glucose handling in X-linked hypophosphataemic mice

1991 ◽  
Vol 80 (1) ◽  
pp. 71-76 ◽  
Author(s):  
R. C. Mühlbauer ◽  
H. Fleisch
Keyword(s):  
Plasma Glucose ◽  
Glucose Concentration ◽  
Plasma Glucose Concentration ◽  
Urinary Glucose Excretion ◽  
Renal Handling ◽  
Acute Response ◽  
Altered Glucose ◽  
Urinary Glucose ◽  
Glucagon And Insulin ◽  
Glucose Excretion

1. The renal handling of glucose was determined in male X-linked hypophosphataemic (Hyp/Y) mice and in control littermates (+/Y) aged 4 months. Plasma glucose concentration and urinary glucose excretion were measured before and during stepwise increase in glycaemia induced by an acute infusion of glucose. The relationship between plasma glucose concentration and urinary glucose excretion was monitored per ml of glomerular filtrate in mice fed high and low phosphate diets. 2. Hyp/Y mice fed the high phosphate diet showed a significantly higher glucosuria compared with +/Y littermates. When glycaemia was increased, Hyp/Y mice developed frank glucosuria earlier than +/Y animals. In Hyp/Y mice we could not find a threshold below which virtually no glucose was excreted in the urine, whereas this was clearly visible in +/Y mice. These differences persisted in animals fed the low phosphate diet. 3. Using the acute response to the glucoregulatory hormones, glucagon and insulin, administered exogenously, we found that the regulation of plasma glucose concentration did not differ between Hyp/Y and +/Y mice. 4. The significantly lower plasma glucose concentration observed in Hyp/Y as compared with +/Y mice decreased further during fasting. 5. We conclude that the renal reabsorptive capacity for glucose is defective in Hyp/Y mice and their low plasma glucose concentration may be explained by the renal leak. Therefore the X-linked phosphataemic mouse appears not only to be characterized by a defect in renal phosphate and calcium reabsorption but also by an altered glucose reabsorption.

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