scholarly journals Identification and comparison of insulin pharmacokinetics injected with a new 4-mm needle vs 6- and 8-mm needles accounting for endogenous insulin and C-peptide secretion kinetics in non-diabetic adult males

2013 ◽  
Vol 4 (3) ◽  
pp. 287-296 ◽  
Author(s):  
Takahisa Hirose ◽  
Takeshi Ogihara ◽  
Shusaku Tozaka ◽  
Sami Kanderian ◽  
Hirotaka Watada
Keyword(s):  
Adult Males ◽  
C Peptide ◽  
Insulin Pharmacokinetics ◽  
Endogenous Insulin ◽  
Peptide Secretion

Impact of Endogenous Insulin Adjustment Methods on the Assessment of Insulin Pharmacokinetics and Pharmacodynamics

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 1051-P
Author(s):  
SANG M. CHUNG ◽  
JUSTIN A. PENZENSTADLER ◽  
MANOJ KHURANA ◽  
CHANDRAHAS SAHAJWALLA
Keyword(s):  
Pharmacokinetics And Pharmacodynamics ◽  
Insulin Pharmacokinetics ◽  
Endogenous Insulin

Influence of residual C-peptide secretion on nocturnal serum TSH peak in well-controlled diabetic patients

1997 ◽  
Vol 47 (3) ◽  
pp. 305-310 ◽  
Author(s):  
V. Coiro ◽  
R. Volpi ◽  
C. Marchesi ◽  
L. Capretti ◽  
G. Speroni ◽  
...  
Keyword(s):  
Diabetic Patients ◽  
C Peptide ◽  
Peptide Secretion ◽  
Serum Tsh

scholarly journals Comparison of proinsulin and C-peptide secretion in healthy versus long-standing type 1 diabetes mellitus cohorts: A pilot study

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0207065 ◽  
Author(s):  
Catherine A. Sullivan ◽  
Jose M. Cacicedo ◽  
Iniya Rajendran ◽  
Devin W. Steenkamp
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Pilot Study ◽  
Type 1 Diabetes Mellitus ◽  
C Peptide ◽  
Peptide Secretion

scholarly journals The effect of oral and intravenous dextrose on C-peptide secretion in ponies1

2016 ◽  
Vol 94 (2) ◽  
pp. 574-580 ◽  
Author(s):  
M. A. de Laat ◽  
J. J. van Haeften ◽  
M. N. Sillence
Keyword(s):  
C Peptide ◽  
Peptide Secretion ◽  
Intravenous Dextrose

C-peptide secretion in calcific tropical pancreatic diabetes

Metabolism ◽  
1986 ◽  
Vol 35 (9) ◽  
pp. 814-817 ◽  
Author(s):  
Sathit Vannasaeng ◽  
Wannee Nitiyanant ◽  
Apichati Vichayanrat ◽  
Sirirat Ploybutr ◽  
Sumon Harnthong
Keyword(s):  
C Peptide ◽  
Pancreatic Diabetes ◽  
Tropical Pancreatic Diabetes ◽  
Peptide Secretion

Effect of GLP-1 and GIP on C-peptide secretion after glucagon or mixed meal tests: Significance in assessing B-cell function in diabetes

2017 ◽  
Vol 33 (6) ◽  
pp. e2899
Author(s):  
C. Guglielmi ◽  
R. Del Toro ◽  
A. Lauria ◽  
A.R. Maurizi ◽  
S. Fallucca ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Function ◽  
Mixed Meal ◽  
C Peptide ◽  
B Cell Function ◽  
Peptide Secretion

Feedback inhibition by biosynthetic human insulin of insulin release in healthy human subjects

1982 ◽  
Vol 243 (6) ◽  
pp. E476-E482 ◽  
Author(s):  
W. K. Waldhausl ◽  
S. Gasic ◽  
P. Bratusch-Marrain ◽  
A. Korn ◽  
P. Nowotny
Keyword(s):  
Insulin Release ◽  
Human Insulin ◽  
Clearance Rate ◽  
Feedback Inhibition ◽  
Insulin Clearance ◽  
Metabolic Clearance ◽  
C Peptide ◽  
Endogenous Insulin ◽  
Hepatic Insulin Clearance ◽  
Biosynthetic Human Insulin

To determine the impact of biosynthetic human insulin (BHI) on endogenous insulin release, splanchnic output and arterial concentrations of C-peptide were measured in eight healthy men after intravenous administration of 0, 0.5, 1.25, U BHI . m-2 . h-1 for 70 min each. Euglycemia was maintained by a variable glucose infusion. Arterial levels of serum insulin were 48 +/- 6 pmol/liter before and 135 +/- 12, 265 +/- 18, and 593 +/- 47 pmol/liter after BHI infusion. Splanchnic C-peptide output was reduced by BHI infusion from 88 +/- 10 pmol/min before to 50 +/- 9, 28 +/- 10, and 18 +/- 16 pmol/min (P less than 0.0025). Simultaneously, arterial concentrations of C-peptide fell from 539 +/- 54 pmol/liter by 29 and 43% when 1.25 and 2.5 U . m-2 . h-1 of BHI were administered. Hepatic insulin uptake was directly related with BHI infusion rate (r = 0.88) and rose during BHI administration from a basal value of 58 +/- 7 to an uptake of 265 +/- 31 pmol/min when 2.5 U . m-2 . h-1 were infused (P less than 0.0005). Basal hepatic insulin clearance was 4.75 +/- 0.60 ml . kg-1 . min-1 and remained unchanged after BHI infusion as did hepatic fractional extraction of insulin, which was 61 +/- 4% in the basal state. Metabolic clearance rate of immunoreactive insulin (MCRi) was dose-dependently reduced by BHI infusion, whereas the relative share of hepatic insulin clearance in total MCRi rose simultaneously (P less than 0.01). We conclude that feedback inhibition of endogenous insulin release may play an important role in vivo. Furthermore, it appears that nonhepatic insulin degradation is a saturable phenomenon as total MCRi fell in the presence of its unchanged hepatic clearance rate after the infusion of large amounts of BHI.

scholarly journals A Versatile Model of Microfluidic Perifusion System for the Evaluation of C-Peptide Secretion Profiles: Comparison Between Human Pancreatic Islets and HLSC-Derived Islet-Like Structures

Biomedicines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 26 ◽  
Author(s):  
Yonathan Gomez ◽  
Victor Navarro-Tableros ◽  
Ciro Tetta ◽  
Giovanni Camussi ◽  
Maria Felice Brizzi
Keyword(s):  
High Glucose ◽  
Ex Vivo ◽  
C Peptide ◽  
Healthy Donors ◽  
Secretion Profile ◽  
Lower Amplitude ◽  
Peptide Secretion ◽  
Perifusion System

A robust and easy-to-use tool for the ex vivo dynamic evaluation of pancreatic islet (PI) function is essential for further development of novel cell-based therapeutic approaches to treating diabetes. Here, we developed four different glucose perifusion protocols (GPPs) in a microfluidic perifusion system (MPS), based entirely on commercially available components. After validation, the GPPs were used to evaluate C-peptide secretion profiles of PIs derived from different donors (healthy, obese, and type 2 diabetic) and from human liver stem-cell-derived islet-like structures (HLSC-ILS). Using this device, we demonstrated that PIs derived from healthy donors displayed a physiological C-peptide secretion profile as characterized by the response to (a) different glucose concentrations, (b) consecutive pulses of high-glucose concentrations, (c) a glucose threshold ranging from 5–8 mM, and (d) a constant high-glucose perifusion in a biphasic manner. Moreover, we were able to detect a dysregulated secretion profile in PIs derived from both obese and type 2 diabetes mellitus (T2DM) donors. Finally, we also evaluated the kinetic secretion profiles of HLSC-ILS, demonstrating that, nonetheless, with a lower amplitude of secretion compared to PI derived from healthy donors, they were already glucose-responsive on day seven post-differentiation. In conclusion, we have provided evidence that our MPS is a versatile device and may represent a valuable tool to study insulin-producing cells in vitro.

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