scholarly journals Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy

2022 ◽  
Author(s):  
Mengmeng Jiang ◽  
Hai Xie ◽  
Chaoyang Zhang ◽  
Tianqin Wang ◽  
Haibin Tian ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Microglia Activation ◽  
Signalling Pathway

Retinal Astrocytes and Microglia Activation in Diabetic Retinopathy Rhesus Monkey Models

2021 ◽  
pp. 1-7
Author(s):  
Yu Xia ◽  
Qihui Luo ◽  
Jingfei Chen ◽  
Chao Huang ◽  
Asad Jahangir ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Rhesus Monkey ◽  
Microglia Activation ◽  
Retinal Astrocytes

scholarly journals Fenofibrate suppresses cellular metabolic memory of high glucose in diabetic retinopathy via a sirtuin 1-dependent signalling pathway

2015 ◽  
Vol 12 (4) ◽  
pp. 6112-6118 ◽  
Author(s):  
SHUZHI ZHAO ◽  
JUN LI ◽  
NA WANG ◽  
BINGQING ZHENG ◽  
TAO LI ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
High Glucose ◽  
Signalling Pathway ◽  
Sirtuin 1 ◽  
Metabolic Memory

scholarly journals Downregulation of MicroRNA 29a/b exacerbated diabetic retinopathy by impairing the function of Müller cells via Forkhead box protein O4

2018 ◽  
Vol 15 (3) ◽  
pp. 214-222 ◽  
Author(s):  
Jiayu Zhang ◽  
Liang Wu ◽  
Jiawei Chen ◽  
Sisi Lin ◽  
Daqiu Cai ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Retinopathy ◽  
Sonic Hedgehog ◽  
Müller Cells ◽  
Signalling Pathway ◽  
Luciferase Reporter ◽  
Muller Cells ◽  
Forkhead Box ◽  
Relationship Of ◽  
Endothelial Growth Factor

Background: Diabetic retinopathy is a neurological disease, which can lead to blindness in severe cases. The pathogenesis underlying diabetic retinopathy is unclear. The aim of this study was to explore the role of dysregulated microRNA 29a/b in the onset and progression of diabetic retinopathy. Methods: Diabetes mellitus was induced in rats using 60 mg/kg of streptozotocin. Glucose (5.5 and 25 mM) was used to stimulate rat retinal Müller cells. Real-time polymerase chain reaction and Western blot analyses were used to determine gene expression. A luciferase reporter assay was conducted to validate the relationship of microRNA 29a/b with glioma-associated oncogene homolog 1 and Forkhead box protein O4. Results: The expression of microRNA 29a/b and glutamine synthetase decreased in both diabetes mellitus rats and rat retinal Müller cells stimulated with high glucose, whereas the expression of sonic hedgehog, glioma-associated oncogene homolog 1, glial fibrillary acidic protein, and vascular endothelial growth factor, as well as the content of glutamate, increased. Dysregulated microRNA 29a/b was directly regulated by the sonic hedgehog–glioma-associated oncogene homolog 1 signalling pathway, and microRNA 29a and microRNA 29b targeted Forkhead box protein O4 and regulated its expression. Conclusion: Downregulation of microRNA 29a/b, mediated by the sonic hedgehog–glioma-associated oncogene homolog 1 signalling pathway, exacerbated diabetic retinopathy by upregulating Forkhead box protein O4.

scholarly journals miR-23b-3p induces the cellular metabolic memory of high glucose in diabetic retinopathy through a SIRT1-dependent signalling pathway

Diabetologia ◽  
2015 ◽  
Vol 59 (3) ◽  
pp. 644-654 ◽  
Author(s):  
Shuzhi Zhao ◽  
Tao Li ◽  
Jun Li ◽  
Qianyi Lu ◽  
Changjing Han ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
High Glucose ◽  
Signalling Pathway ◽  
Metabolic Memory

Involvement of HMGB1 mediated signalling pathway in diabetic retinopathy: evidence from type 2 diabetic rats and ARPE-19 cells under diabetic condition

2013 ◽  
Vol 97 (12) ◽  
pp. 1598-1603 ◽  
Author(s):  
Xiao-Li Chen ◽  
Xue-Dong Zhang ◽  
Ying-Yuan Li ◽  
Xue-Mei Chen ◽  
De-Rong Tang ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Diabetic Rats ◽  
Signalling Pathway ◽  
Type 2 Diabetic

scholarly journals Luteolin, an aryl hydrocarbon receptor antagonist, alleviates diabetic retinopathy by regulating the NLRP/NOX4 signalling pathway: Experimental and molecular docking study

2021 ◽  
Author(s):  
Y. Yang ◽  
M. Zhou ◽  
H. Liu
Keyword(s):  
Oxidative Stress ◽  
Diabetic Retinopathy ◽  
Molecular Docking ◽  
Blood Glucose ◽  
Docking Study ◽  
Treated Group ◽  
Signalling Pathway ◽  
Fundus Photography ◽  
Molecular Docking Study ◽  
Apoptotic Proteins

AbstractObjectiveThe present report evaluates the protective effects of luteolin against diabetic retinopathy (DR).Materials and methodsDiabetes was induced in rats by i.p. administration of 60 mg/kg of streptozotocin (STZ), followed by treatment with luteolin for 4 weeks. The effects of luteolin were determined based on the blood glucose and cytokine levels, and parameters of oxidative stress in retinal tissue of DR rats. The diameter of retinal vessels was estimated by fundus photography. A Western blot assay was used to determine the expression of apoptotic proteins and Nod-like receptor 3 (NLRP3) pathway proteins in the retina of DR rats. A molecular docking study was performed to evaluate the interaction between luteolin and NLRP3.ResultsThe level of blood glucose was reduced in the luteolin-treated group compared with the DR group. Reductions in cytokines and oxidative stress were observed in the retinal tissues of the luteolin-treated group relative to the DR group. Moreover, treatment with luteolin reduced the expression of NLRP1, NOX4, TXNIP, and NLRP3 proteins, and ameliorated the altered expression of apoptotic proteins in the retina of DR rats.ConclusionIn conclusion, luteolin prevents retinal apoptosis in DR rats by regulating the NLRP/NOX4 signalling pathway.

scholarly journals Elevated Glucose and Interleukin-1β Differentially Affect Retinal Microglial Cell Proliferation

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Filipa I. Baptista ◽  
Célia A. Aveleira ◽  
Áurea F. Castilho ◽  
António F. Ambrósio
Keyword(s):  
Cell Proliferation ◽  
Diabetic Retinopathy ◽  
Cell Cultures ◽  
High Glucose ◽  
Neural Cell ◽  
Microglia Activation ◽  
Retinal Cell ◽  
Diabetic Patients ◽  
Low Grade ◽  
Activated State

Diabetic retinopathy is considered a neurovascular disorder, hyperglycemia being considered the main risk factor for this pathology. Diabetic retinopathy also presents features of a low-grade chronic inflammatory disease, including increased levels of cytokines in the retina, such as interleukin-1 beta (IL-1β). However, how high glucose and IL-1β affect the different retinal cell types remains to be clarified. In retinal neural cell cultures, we found that IL-1β and IL-1RI are present in microglia, macroglia, and neurons. Exposure of retinal neural cell cultures to high glucose upregulated both mRNA and protein levels of IL-1β. High glucose decreased microglial and macroglial cell proliferation, whereas IL-1β increased their proliferation. Interestingly, under high glucose condition, although the number of microglial cells decreased, they showed a less ramified morphology, suggesting a more activated state, as supported by the upregulation of the levels of ED-1, a marker of microglia activation. In conclusion, IL-1β might play a key role in diabetic retinopathy, affecting microglial and macroglial cells and ultimately contributing to neural changes observed in diabetic patients. Particularly, since IL-1β has an important role in retinal microglia activation and proliferation under diabetes, limiting IL-1β-triggered inflammatory processes may provide a new therapeutic strategy to prevent the progression of diabetic retinopathy.

scholarly journals Prostaglandin E2/EP2 receptor signalling pathway promotes diabetic retinopathy in a rat model of diabetes

Diabetologia ◽  
2018 ◽  
Vol 62 (2) ◽  
pp. 335-348 ◽  
Author(s):  
Man Wang ◽  
Yangningzhi Wang ◽  
Tianhua Xie ◽  
Pengfei Zhan ◽  
Jian Zou ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Prostaglandin E2 ◽  
Rat Model ◽  
Signalling Pathway ◽  
Receptor Signalling ◽  
Ep2 Receptor
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