scholarly journals MicroRNA‐381 in human cancer: Its involvement in tumour biology and clinical applications potential

2022 ◽  
Author(s):  
Huanhuan Sha ◽  
Yujie Gan ◽  
Feng Xu ◽  
Yue Zhu ◽  
Renrui Zou ◽  
...  
Keyword(s):  
Human Cancer ◽  
Clinical Applications ◽  
Tumour Biology

Differentiation therapy of human cancer: basic science and clinical applications

2001 ◽  
Vol 90 (2-3) ◽  
pp. 105-156 ◽  
Author(s):  
Magdalena Leszczyniecka ◽  
Terry Roberts ◽  
Paul Dent ◽  
Steven Grant ◽  
Paul B Fisher
Keyword(s):  
Basic Science ◽  
Human Cancer ◽  
Clinical Applications ◽  
Differentiation Therapy

scholarly journals Bioengineered 3D models of human pancreatic cancer recapitulate in vivo tumour biology

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
David Osuna de la Peña ◽  
Sara Maria David Trabulo ◽  
Estelle Collin ◽  
Ying Liu ◽  
Shreya Sharma ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Human Cancer ◽  
Human Pancreatic Cancer ◽  
Ductal Adenocarcinoma ◽  
Patient Specific ◽  
In Vivo Models ◽  
Mesenchymal Transition ◽  
Matrix Deposition ◽  
Tumour Biology

AbstractPatient-derived in vivo models of human cancer have become a reality, yet their turnaround time is inadequate for clinical applications. Therefore, tailored ex vivo models that faithfully recapitulate in vivo tumour biology are urgently needed. These may especially benefit the management of pancreatic ductal adenocarcinoma (PDAC), where therapy failure has been ascribed to its high cancer stem cell (CSC) content and high density of stromal cells and extracellular matrix (ECM). To date, these features are only partially reproduced ex vivo using organoid and sphere cultures. We have now developed a more comprehensive and highly tuneable ex vivo model of PDAC based on the 3D co-assembly of peptide amphiphiles (PAs) with custom ECM components (PA-ECM). These cultures maintain patient-specific transcriptional profiles and exhibit CSC functionality, including strong in vivo tumourigenicity. User-defined modification of the system enables control over niche-dependent phenotypes such as epithelial-to-mesenchymal transition and matrix deposition. Indeed, proteomic analysis of these cultures reveals improved matrisome recapitulation compared to organoids. Most importantly, patient-specific in vivo drug responses are better reproduced in self-assembled cultures than in other models. These findings support the use of tuneable self-assembling platforms in cancer research and pave the way for future precision medicine approaches.

The Mouse Tumour Biology Database: an Online Resource for Mouse Models of Human Cancer

2006 ◽  
pp. 141-153
Author(s):  
Carol J. Bult ◽  
Debra M. Krupke ◽  
Matthew J. Vincent ◽  
Theresa Allio ◽  
John P. Sundberg ◽  
...  
Keyword(s):  
Mouse Models ◽  
Human Cancer ◽  
Tumour Biology ◽  
Online Resource ◽  
Mouse Tumour

Repeatability and reproducibility of desorption electrospray ionization-mass spectrometry (DESI-MS) for the imaging analysis of human cancer tissue: a gateway for clinical applications

2015 ◽  
Vol 7 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Nima Abbassi-Ghadi ◽  
Emrys A. Jones ◽  
Kirill A. Veselkov ◽  
Juzheng Huang ◽  
Sacheen Kumar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Cancer ◽  
Desorption Electrospray Ionization ◽  
Clinical Applications ◽  
Cancer Tissue ◽  
Ionization Mass ◽  
Imaging Analysis ◽  
Desi Ms ◽  
Repeatability And Reproducibility ◽  
Human Cancer Tissue

The repeatability and reproducibility of DESI-MS for the measurement of lipid ion intensities in human cancer tissue is 22 ± 7% and 18 ± 8%, respectively.

scholarly journals Molecular mechanisms and clinical applications of miR-22 in regulating malignant progression in human cancer (Review)

2016 ◽  
Vol 50 (2) ◽  
pp. 345-355 ◽  
Author(s):  
Jingyu Wang ◽  
Yuan Li ◽  
Meiman Ding ◽  
Honghe Zhang ◽  
Xiaoming Xu ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Human Cancer ◽  
Clinical Applications ◽  
Malignant Progression ◽  
Cancer Review

DNA Microarrays in Clinical Oncology

2002 ◽  
Vol 20 (7) ◽  
pp. 1932-1941 ◽  
Author(s):  
Sridhar Ramaswamy ◽  
Todd R. Golub
Keyword(s):  
Gene Expression ◽  
Clinical Oncology ◽  
Expression Profiling ◽  
Dna Microarrays ◽  
Human Cancer ◽  
Clinical Applications ◽  
Cancer Gene ◽  
Comprehensive Understanding ◽  
Aberrant Gene Expression ◽  
Aberrant Gene

ABSTRACT: Aberrant gene expression is critical for tumor initiation and progression. However, we lack a comprehensive understanding of all genes that are aberrantly expressed in human cancer. Recently, DNA microarrays have been used to obtain global views of human cancer gene expression and to identify genetic markers that might be important for diagnosis and therapy. We review clinical applications of these novel tools, discuss some important recent studies, identify promising avenues of research in this emerging field of study, and discuss the likely impact that expression profiling will have on clinical oncology.

scholarly journals Recent Advances in p53 Research and Cancer Treatment

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Kazufumi Suzuki ◽  
Hisahiro Matsubara
Keyword(s):  
Cell Cycle ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Human Cancer ◽  
Basic Function ◽  
Clinical Applications ◽  
Mutant P53 ◽  
Cycle Arrest ◽  
Recent Advances ◽  
Response To Stress

TP53, encoding p53, is one of the most famous tumor suppressor genes. The majority of human cancers demonstrate the inactivation of the p53 pathway. Mutant p53 not only, no longer, functions as a tumor suppressor but can also exert tumor-promoting effects. The basic function of p53 is to respond to cellular stress. We herein review the recent advances in p53 research and focus on apoptosis, cell cycle arrest, and senescence in response to stress. We also review the clinical applications of p53-based therapy for human cancer.

Specimen Preparation for Clinical Applications of X-Ray Microanalysis

1990 ◽  
Vol 48 (2) ◽  
pp. 328-329
Author(s):  
J.D. Shelburne ◽  
G.M. Roomans
Keyword(s):  
Carbon Coating ◽  
Clinical Applications ◽  
Point Of View ◽  
Practical Significance ◽  
Sweat Test ◽  
Specimen Preparation ◽  
Tissue Preparation ◽  
X Ray ◽  
Gallbladder Stones ◽  
Clinical Problems

Proper preparative procedures are a prerequisite for the validity of the results of x-ray microanalysis of biological tissue. Clinical applications of x-ray microanalysis are often concerned with diagnostic problems and the results may have profound practical significance for the patient. From this point of view it is especially important that specimen preparation for clinical applications is carried out correctly.Some clinical problems require very little tissue preparation. Hair, nails, and kidney and gallbladder stones may be examined and analyzed after carbon coating. High levels of zinc or copper in hair may be indicative of dermatological or systemic diseases. Nail clippings may be analyzed (as an alternative to the more conventional sweat test) to confirm a diagnosis of cystic fibrosis. X-ray microanalysis in combination with scanning electron microscopy has been shown to be the most reliable method for the identification of the components of kidney or gallbladder stones.A quantitatively very important clinical application of x-ray microanalysis is the identification and quantification of asbestos and other exogenous particles in lung.

Reduction of Potassium in Kidney Proximal Tubules to Aid in Electron Probe X-Ray Microanalysis of Calcium

1985 ◽  
Vol 43 ◽  
pp. 474-475
Author(s):  
A. LeFurgey ◽  
P. Ingram ◽  
L.J. Mandel
Keyword(s):  
Smooth Muscle ◽  
Proximal Tubule ◽  
Electron Probe ◽  
Clinical Applications ◽  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Target Cells ◽  
X Ray ◽  
Freeze Dried

For quantitative determination of subcellular Ca distribution by electron probe x-ray microanalysis, decreasing (and/or eliminating) the K content of the cell maximizes the ability to accurately separate the overlapping K Kß and Ca Kα peaks in the x-ray spectra. For example, rubidium has been effectively substituted for potassium in smooth muscle cells, thus giving an improvement in calcium measurements. Ouabain, a cardiac glycoside widely used in experimental and clinical applications, inhibits Na-K ATPase at the cell membrane and thus alters the cytoplasmic ion (Na,K) content of target cells. In epithelial cells primarily involved in active transport, such as the proximal tubule of the rabbit kidney, ouabain rapidly (t1/2= 2 mins) causes a decrease2 in intracellular K, but does not change intracellular total or free Ca for up to 30 mins. In the present study we have taken advantage of this effect of ouabain to determine the mitochondrial and cytoplasmic Ca content in freeze-dried cryosections of kidney proximal tubule by electron probe x-ray microanalysis.

Sign in / Sign up

Export Citation Format

Share Document