Systematic proteomics analysis of lysine acetylation reveals critical features of placental proteins in pregnant women with preeclampsia

Comparative Proteomics Analysis of Placenta from Pregnant Women with Intrahepatic Cholestasis of Pregnancy

PLoS ONE ◽

10.1371/journal.pone.0083281 ◽

2013 ◽

Vol 8 (12) ◽

pp. e83281 ◽

Cited By ~ 11

Author(s):

Ting Zhang ◽

Yueshuai Guo ◽

Xuejiang Guo ◽

Tao Zhou ◽

Daozhen Chen ◽

...

Keyword(s):

Pregnant Women ◽

Intrahepatic Cholestasis ◽

Comparative Proteomics ◽

Intrahepatic Cholestasis Of Pregnancy ◽

Proteomics Analysis ◽

Cholestasis Of Pregnancy

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Quantitative proteomics analysis reveals alterations of lysine acetylation in mouse testis in response to heat shock and X-ray exposure

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/j.bbapap.2017.11.011 ◽

2018 ◽

Vol 1866 (3) ◽

pp. 464-472 ◽

Cited By ~ 6

Author(s):

Chengmei Xie ◽

Hongyan Shen ◽

Hui Zhang ◽

Jinting Yan ◽

Yang Liu ◽

...

Keyword(s):

Heat Shock ◽

Quantitative Proteomics ◽

Mouse Testis ◽

Lysine Acetylation ◽

Proteomics Analysis ◽

X Ray

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Proteomics Analysis of Serum Proteins in Gestational Diabetes

10.21203/rs.3.rs-313262/v1 ◽

2021 ◽

Author(s):

Jianhua Li ◽

Lin Lu ◽

Xinping Xie ◽

Xiaofeng Dai ◽

Lihong Chen ◽

...

Keyword(s):

Gestational Diabetes ◽

Pregnant Women ◽

High Performance ◽

Bioinformatics Analysis ◽

Serum Proteins ◽

Differentially Expressed ◽

Differentially Expressed Proteins ◽

Proteomics Analysis ◽

Serum Proteomics ◽

Parallel Reaction Monitoring

Abstract Purpose The purpose of this study was to screen serum proteins for biomarkers of gestational diabetes mellitus (GDM) and to investigate its pathogenesis by analyzing the differences in serum proteomics between pregnant women with GDM and healthy pregnant women. Methods High performance liquid chromatography-mass spectrometry was used to identify differentially expressed serum proteins between pregnant women with GDM and healthy pregnant women, and bioinformatics analysis was then performed on the identified proteins. Results A total of 1152 quantifiable proteins were detected; among them, 15 were up-regulated in the serum of GDM pregnant women, while 26 were down-regulated. The subsequent parallel reaction monitoring (PRM) assay validated the expression levels of 12 out of 41 differentially expressed proteins. Moreover, bioinformatics analysis revealed that the differentially expressed proteins are involved in multiple biological processes and signaling pathways related to lipid metabolism, glycan degradation, immune response, and platelet aggregation. Conclusions This study identified 41 serum proteins with differential expression between pregnant women with GDM and healthy pregnant women, providing new candidate molecules for elucidating GDM pathogenesis and screening therapeutic targets.

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The challenge of detecting modifications on proteins

Essays in Biochemistry ◽

10.1042/ebc20190055 ◽

2020 ◽

Vol 64 (1) ◽

pp. 135-153 ◽

Cited By ~ 1

Author(s):

Lauren Elizabeth Smith ◽

Adelina Rogowska-Wrzesinska

Keyword(s):

Mass Spectrometry ◽

Protein Function ◽

Chemical Properties ◽

Lysine Acetylation ◽

Mass Determination ◽

Post Translational Modifications ◽

Site Specific ◽

The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

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