scholarly journals Human platelet lysate as a replacement for fetal bovine serum in human corneal stromal keratocyte and fibroblast culture

2021 ◽  
Author(s):  
Nina Seidelmann ◽  
Daniela F. Duarte Campos ◽  
Malena Rohde ◽  
Sandra Johnen ◽  
Sabine Salla ◽  
...  
Keyword(s):  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Fibroblast Culture ◽  
Human Platelet Lysate ◽  
Fetal Bovine

Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation?

Biomaterials ◽  
2016 ◽  
Vol 76 ◽  
pp. 371-387 ◽  
Author(s):  
Thierry Burnouf ◽  
Dirk Strunk ◽  
Mickey B.C. Koh ◽  
Katharina Schallmoser
Keyword(s):  
Human Cell ◽  
Gold Standard ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine ◽  
Cell Propagation

Human platelet lysate produced at industrial scale for use as fetal bovine serum replacement in cell manufacturing protocols

Cytotherapy ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. S41
Author(s):  
Yiwei Ma ◽  
Samantha Reilly ◽  
Stephen E. Fischer ◽  
Mariluz Henshaw ◽  
Jan Pierce ◽  
...  
Keyword(s):  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Industrial Scale ◽  
Serum Replacement ◽  
Cell Manufacturing ◽  
Human Platelet Lysate ◽  
Fetal Bovine

scholarly journals Human platelet lysate to substitute fetal bovine serum in hMSC expansion for translational applications: a systematic review

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
M. Guiotto ◽  
W. Raffoul ◽  
A. M. Hart ◽  
M. O. Riehle ◽  
P. G. di Summa
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Search Algorithm ◽  
Platelet Lysate ◽  
Pubmed Database ◽  
Human Platelet Lysate ◽  
Fetal Bovine

Abstract Background Foetal bovine serum (FBS), is the most commonly used culture medium additive for in vitro cultures, despite its undefined composition, its potential immunogenicity and possible prion/zoonotic transmission. For these reasons, significant efforts have been targeted at finding a substitute, such as serum free-media or human platelet-lysates (hPL). Our aim is to critically appraise the state-of-art for hPL in the published literature, comparing its impact with FBS. Materials and methods In June 2019 a systematic search of the entire Web of Science, Medline and PubMed database was performed with the following search terms: (mesenchymal stem cells) AND (fetal bovine serum OR fetal bovine calf) AND (human platelet lysate). Excluded from this search were review articles that were published before 2005, manuscripts in which mesenchymal stem cells (MSCs) were not from human sources, and when the FBS controls were missing. Results Based on our search algorithm, 56 papers were selected. A review of these papers indicated that hMSCs cultured with hPL showed a spindle-shaped elongated morphology, had higher proliferation indexes, similar cluster of differentiation (CD) markers and no significant variation in differentiation lineage (osteocyte, adipocyte, and chondrocyte) compared to those cultured with FBS. Main sources of primary hMSCs were either fat tissue or bone marrow; in a few studies cells isolated from alternative sources showed no relevant difference in their response. Conclusion Despite the difference in medium choice and a lack of standardization of hPL manufacturing, the majority of publications support that hPL was at least as effective as FBS in promoting adhesion, survival and proliferation of hMSCs. We conclude that hPL should be considered a viable alternative to FBS in hMSCs culture—especially with a view for their clinical use.

Comparison of fetal bovine serum and human platelet lysate in cultivation and differentiation of dental pulp stem cells into hepatic lineage cells

2014 ◽  
Vol 88 ◽  
pp. 142-153 ◽  
Author(s):  
Punitha Vasanthan ◽  
Nareshwaran Gnanasegaran ◽  
Vijayendran Govindasamy ◽  
Aimi Naim Abdullah ◽  
Pukana Jayaraman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Dental Pulp Stem Cells ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine
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