scholarly journals Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis

2021 ◽  
Author(s):  
Xingwen Wang ◽  
Jinwen He ◽  
Hong Wang ◽  
Dacheng Zhao ◽  
Bin Geng ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Osteoblast Proliferation ◽  
Fluid Shear ◽  
Proliferation And Apoptosis ◽  
Lncrna Tug1

Fluid shear stress promotes osteoblast proliferation via the Gαq–ERK5 signaling pathway

2016 ◽  
Vol 57 (4) ◽  
pp. 299-306 ◽  
Author(s):  
Zhang Bo ◽  
Geng Bin ◽  
Wang Jing ◽  
Wang Cuifang ◽  
An Liping ◽  
...  
Keyword(s):  
Shear Stress ◽  
Signaling Pathway ◽  
Fluid Shear Stress ◽  
Osteoblast Proliferation ◽  
Fluid Shear

The calcium transient characteristics induced by fluid shear stress affect the osteoblast proliferation

2018 ◽  
Vol 362 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Liyin Yu ◽  
Xiangmiao Wang ◽  
Xiaohang Gao ◽  
Jie Tong ◽  
Jianbao Zhang
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Calcium Transient ◽  
Osteoblast Proliferation ◽  
Fluid Shear ◽  
Transient Characteristics

Fluid shear stress promotes osteoblast proliferation through the NFATc1-ERK5 pathway

2018 ◽  
Vol 60 (2) ◽  
pp. 107-116 ◽  
Author(s):  
Ning Ding ◽  
Bin Geng ◽  
Zhonghao Li ◽  
Quanzeng Yang ◽  
Liang Yan ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Osteoblast Proliferation ◽  
Fluid Shear

scholarly journals Epigenetic Changes During Mechanically Induced Osteogenic Lineage Commitment

2015 ◽  
Vol 137 (2) ◽  
Author(s):  
Julia C. Chen ◽  
Mardonn Chua ◽  
Raymond B. Bellon ◽  
Christopher R. Jacobs
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Shear Stress ◽  
Fluid Shear Stress ◽  
Methylation Status ◽  
Lineage Commitment ◽  
Fluid Shear ◽  
Osteogenic Lineage ◽  
Osteogenic Markers ◽  
Heterochromatin Structure

Osteogenic lineage commitment is often evaluated by analyzing gene expression. However, many genes are transiently expressed during differentiation. The availability of genes for expression is influenced by epigenetic state, which affects the heterochromatin structure. DNA methylation, a form of epigenetic regulation, is stable and heritable. Therefore, analyzing methylation status may be less temporally dependent and more informative for evaluating lineage commitment. Here we analyzed the effect of mechanical stimulation on osteogenic differentiation by applying fluid shear stress for 24 hr to osteocytes and then applying the osteocyte-conditioned medium (CM) to progenitor cells. We analyzed gene expression and changes in DNA methylation after 24 hr of exposure to the CM using quantitative real-time polymerase chain reaction and bisulfite sequencing. With fluid shear stress stimulation, methylation decreased for both adipogenic and osteogenic markers, which typically increases availability of genes for expression. After only 24 hr of exposure to CM, we also observed increases in expression of later osteogenic markers that are typically observed to increase after seven days or more with biochemical induction. However, we observed a decrease or no change in early osteogenic markers and decreases in adipogenic gene expression. Treatment of a demethylating agent produced an increase in all genes. The results indicate that fluid shear stress stimulation rapidly promotes the availability of genes for expression, but also specifically increases gene expression of later osteogenic markers.

Patterns of fluid shear stress determine atherosclerotic lesion size and vulnerability

2006 ◽  
Vol 45 (3) ◽  
pp. e51
Author(s):  
Caroline Cheng ◽  
Dennie Tempel ◽  
Luc van Damme ◽  
Rien van Haperen ◽  
Rob Krams ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Atherosclerotic Lesion ◽  
Lesion Size ◽  
Fluid Shear
Sign in / Sign up

Export Citation Format

Share Document