scholarly journals Harmine targets inhibitor of DNA binding‐2 and activator protein‐1 to promote preosteoclast PDGF‐BB production

2021 ◽  
Author(s):  
Jie Huang ◽  
You‐You Li ◽  
Kun Xia ◽  
Yi‐Yi Wang ◽  
Chun‐Yuan Chen ◽  
...  
Keyword(s):  
Dna Binding ◽  
Activator Protein 1 ◽  
Activator Protein ◽  
Inhibitor Of Dna Binding

scholarly journals Phosphorylation of BATF regulates DNA binding: a novel mechanism for AP-1 (activator protein-1) regulation

2003 ◽  
Vol 374 (2) ◽  
pp. 423-431 ◽  
Author(s):  
Christopher D. DEPPMANN ◽  
Tina M. THORNTON ◽  
Fransiscus E. UTAMA ◽  
Elizabeth J. TAPAROWSKY
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Leucine Zipper ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Activator Protein 1 ◽  
Basic Leucine Zipper ◽  
Activator Protein

BATF is a member of the AP-1 (activator protein-1) family of bZIP (basic leucine zipper) transcription factors that form transcriptionally inhibitory, DNA binding heterodimers with Jun proteins. In the present study, we demonstrate that BATF is phosphorylated in vivo on multiple serine and threonine residues and at least one tyrosine residue. Reverse-polarity PAGE revealed that serine-43 and threonine-48 within the DNA binding domain of BATF are phosphorylated. To model phosphorylation of the BATF DNA binding domain, serine-43 was replaced by an aspartate residue. BATF(S43D) retains the ability to dimerize with Jun proteins in vitro and in vivo, and the BATF(S43D):Jun heterodimer localizes properly to the nucleus of cells. Interestingly, BATF(S43D) functions like wild-type BATF to reduce AP-1-mediated gene transcription, despite the observed inability of the BATF(S43D):Jun heterodimer to bind DNA. These data demonstrate that phosphorylation of serine-43 converts BATF from a DNA binding into a non-DNA binding inhibitor of AP-1 activity. Given that 40% of mammalian bZIP transcription factors contain a residue analogous to serine-43 of BATF in their DNA binding domains, the phosphorylation event described here represents a mechanism that is potentially applicable to the regulation of many bZIP proteins.

scholarly journals Localization of Activator Protein-1 Complex with DNA Binding Activity in Mitochondria of Murine Brain after In VivoTreatment with Kainate

2002 ◽  
Vol 22 (7) ◽  
pp. 2561-2570 ◽  
Author(s):  
Kiyokazu Ogita ◽  
Hiroaki Okuda ◽  
Masahiro Kitano ◽  
Yoshiaki Fujinami ◽  
Kiyokazu Ozaki ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Activity ◽  
Activator Protein 1 ◽  
Activator Protein ◽  
Dna Binding Activity ◽  
Murine Brain

Possible correlation between abilities of a variety of polyamines to increase activator protein-1 DNA binding and to inhibit [3H]spermidine transport in nuclear fractions of murine brain

2003 ◽  
Vol 987 (1) ◽  
pp. 126-130
Author(s):  
Keiji Inoue ◽  
Nobuyuki Kuramoto ◽  
Katsura Takano ◽  
Hideo Taniura ◽  
Katsumi Sakata ◽  
...  
Keyword(s):  
Dna Binding ◽  
Activator Protein 1 ◽  
Activator Protein ◽  
Murine Brain
Sign in / Sign up

Export Citation Format

Share Document