scholarly journals Enhanced Nrf2 up‐regulation by extracellular basic pH in a human skin equivalent system

2021 ◽  
Author(s):  
Gunhyuk Park ◽  
Byeong Cheol Moon ◽  
Dal‐Seok Oh ◽  
Yong‐Ung Kim ◽  
Moon‐Ki Park
Keyword(s):  
Human Skin ◽  
Skin Equivalent ◽  
Equivalent System ◽  
Human Skin Equivalent

Acceleration of Apoptosis by Extracellular Basic pH in a 3D Human Skin Equivalent System

Pharmacology ◽  
2017 ◽  
Vol 100 (1-2) ◽  
pp. 98-104
Author(s):  
Gunhyuk Park ◽  
Dal-Seok Oh ◽  
Yong-ung Kim ◽  
Moon Ki Park
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Healing Process ◽  
B Cell Lymphoma ◽  
Skin Equivalent ◽  
Wound Healing Process ◽  
Equivalent System ◽  
Skin Damage ◽  
Apoptosis Proteins ◽  
Human Skin Equivalent

Previously, we have shown that extracellular basic pH plays a significant role in both the direct and indirect regulation of cellular processes in a wound; this in turn affects the wound-healing process. Several studies have demonstrated the importance of apoptosis modulation in the wound-healing process, especially in removing inflammatory cells and in inhibiting scar formation. However, the effects of extracellular basic pH on wound healing-related skin damage are yet to be examined. Therefore, we investigated the induction of accelerated apoptosis by extracellular basic pH in skin. Apoptosis-related protein levels were measured using an array kit, target protein expression levels were detected by immunostaining, lactate dehydrogenase was analyzed spectrophotometrically, and Annexin V levels were measured by fluorescence staining. Basic pH (8.40) strongly upregulated extrinsic apoptosis proteins (Fas, high temperature requirement A, and p21) and slightly upregulated intrinsic apoptosis proteins (cytochrome c, B-cell lymphoma 2 [Bcl-2], Bcl-2-associated death promoter, and Bcl-2-like protein 4) in a 3D human skin equivalent system. Moreover, basic pH (8.40) induced heat shock protein (HSP) 60 and 70. In addition, basic pH-exposed Fas- and HSP60-knockdown cells showed significantly decreased levels of apoptosis. Taken together, these results indicate that extracellular basic pH increases early-stage apoptosis through Fas/FasL via modulation of HSP60 and HSP70.

Desmosomal distribution in the epidermis of a non-contracting human skin equivalent

1992 ◽  
Vol 50 (1) ◽  
pp. 896-897
Author(s):  
L.X. Oakford ◽  
S.D. Dimitrijevich ◽  
R. Gracy
Keyword(s):  
Human Skin ◽  
Basal Layer ◽  
Skin Equivalent ◽  
Tissue Component ◽  
Intact Skin ◽  
Similar Sequence ◽  
Sequence Of Events ◽  
Suprabasal Keratinocytes ◽  
Human Skin Equivalent

In intact skin the epidermal layer is a dynamic tissue component which is maintained by a basal layer of mitotically active cells. The protective upper epidermis, the stratum corneum, is generated by differentiation of the suprabasal keratinocytes which eventually desquamate as anuclear comeocytes. A similar sequence of events is observed in vitro in the non-contracting human skin equivalent (HSE) which was developed in this lab (1). As a part of the definition process for this model of living skin we are examining its ultrastructural features. Since desmosomes are important in maintaining cell-cell interactions in stratified epithelia their distribution in HSE was examined.

Transformative Encounters and Uncanny Intimacies: Biotechnology and Contemporary Art – A Practitioner's Approach and Perspective

Somatechnics ◽  
2012 ◽  
Vol 2 (2) ◽  
pp. 263-283 ◽  
Author(s):  
Svenja J. Kratz
Keyword(s):  
Tissue Culture ◽  
Human Skin ◽  
Contemporary Art ◽  
Skin Equivalent ◽  
Biomedical Innovation ◽  
Equivalent Models ◽  
Science Projects ◽  
Insight Into ◽  
Human Skin Equivalent

Abstract: Presented from an ArtScience practitioner's perspective, this paper provides an overview of Svenja Kratz's experience working as an artist within the area of cell and tissue culture at QUT's Institute of Health and Biomedical Innovation (IHBI). Using The Absence of Alice, a multi-medium exhibition based on the experience of culturing cells, as a case study, the paper gives insight into the artist's approach to working across art and science and how ideas, processes, and languages from each discipline can intermesh and extend the possibilities of each system. The paper also provides an overview of her most recent artwork, The Human Skin Equivalent/Experience Project, which involves the creation of personal jewellery items incorporating human skin equivalent models grown from the artist's skin and participant cells. Referencing this project, and other contemporary bioart works, the value of ArtScience is discussed, focusing in particular on the way in which cross-art-science projects enable an alternative voice to enter into scientific dialogues and have the potential to yield outcomes valuable to both disciplines.

Development of a Three-Dimensional Human Skin Equivalent Wound Model for Investigating Novel Wound Healing Therapies

2010 ◽  
Vol 16 (5) ◽  
pp. 1111-1123 ◽  
Author(s):  
Yan Xie ◽  
Simone C. Rizzi ◽  
Rebecca Dawson ◽  
Emily Lynam ◽  
Sean Richards ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Three Dimensional ◽  
Skin Equivalent ◽  
Wound Model ◽  
Human Skin Equivalent

scholarly journals Type 2 Helper T-Cell Cytokines Induce Morphologic and Molecular Characteristics of Atopic Dermatitis in Human Skin Equivalent

2011 ◽  
Vol 178 (5) ◽  
pp. 2091-2099 ◽  
Author(s):  
Marijke Kamsteeg ◽  
Mieke Bergers ◽  
Roelie de Boer ◽  
Patrick L.J.M. Zeeuwen ◽  
Stanleyson V. Hato ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
T Cell ◽  
Human Skin ◽  
Skin Equivalent ◽  
Molecular Characteristics ◽  
Helper T Cell ◽  
Human Skin Equivalent
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